Gambierdiscus in the main land of Japan

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1 Gambierdiscus in the main land of Japan Takuo Omura and Yasuwo Fukuyo LASC Co., LTD. and The University of Tokyo

2 1. Introduction CFP causative species Photomicrograph by Y. Fukuyo Photomicrograph by Y. Fukuyo Gambierdiscus toxicus

3 1. Introduction Map of CFP CFP hot spots CFP related area

4 1. Introduction 本州における最近のシガテラ事例 Newspapers of Ciguatera in main land Japan

5 2 Ciguatera Fish Poisoning (CFP) CFP incidents by eating Spotted knifejaw (Oplegnathus punctatus) Date Location Eating party Patients 1988, March 1992, March 1998, April 1998, August 1999, November Okinawa (Naha) Okinawa (Gusikawa) Miyazaki (Miyazaki) Kagoshima (Ooshima) Chiba (Katsuura)

6 2 Ciguatera Fish Poisoning (CFP) CFP incidents by eating Spotted knifejaw (Oplegnathus punctatus) Date Location Eating party Patients 1988, March Okinawa (Naha) , March Okinawa (Gusikawa) , April Miyazaki (Miyazaki) , August Kagoshima (Ooshima) 19 4 Chiba 1999, November Chiba (Katsuura) 12 Miyazaki 10 Kagoshima Okinawa

7 2 Ciguatera Fish Poisoning (CFP) Recent data of CFP in main land of Japan 2006, 10 June Ibaraki Pref. 4 patients Two-spot red snapper (Lutjanus bohar )( Meuniere ) 2007, April Shizuoka Pref. 7 patients Spotted knifejaw (Oplegnathus punctatus) 2007, June Osaka Pref. 9 patients Spotted knifejaw (Oplegnathus punctatus) From Wakayama Pref. by fishing (Sashimi & boiling) 2008, 6 July Aichi Pref. 3 patients Spotted knifejaw (Oplegnathus punctatus) From Mie Pref. by fishing (Sashimi & boiling) 2009, 12 December Hyogo Pref. 4 patients Two-spot red snapper (Lutjanus bohar ) From Kagoshima Pref. by fishing (Hot pot & french-fry)

8 2 Ciguatera Fish Poisoning (CFP) Recent data of CFP in main land of Japan 2006, 10 June Ibaraki Pref. 4 patients Two-spot red snapper (Lutjanus bohar )( Meuniere ) 2007, April Shizuoka Pref. 7 patients Spotted knifejaw (Oplegnathus punctatus) 2007, June Osaka Pref. 9 patients Spotted knifejaw (Oplegnathus punctatus) From Wakayama Pref. by fishing (Sashimi & boiling) Osaka Hyogo Ibaraki 2008, 6 July Aichi Pref. 3 patients Spotted knifejaw (Oplegnathus punctatus) From Mie Pref. by fishing (Sashimi & boiling) Miyazaki Kagoshima 2009, 12 December Hyogo Pref. 4 patients Two-spot red snapper (Lutjanus bohar ) From Kagoshima Pref. by fishing (Hot pot & french-fry) Okinawa Aichi Chiba Shizuoka

9 3 Distribution of CFP causative species in Japan Gambierdiscus founding area Recent information(2008-) Before information(2006-) Old information(1980-)

10 3. Distribution of CFP causative species in Japan How to collect seaweed samples

11 3. Distribution of CFP causative species in Japan Recommended sampling of seaweeds Small volume of seaweeds is put into a plastic bag with a zipper or plastic bottle for each species., respectively.

12 3. Distribution of CFP causative species in Japan Sample making for microscopic observation times shakes 2 Sieve(180 µm mesh) 3 Sieve(32 µm mesh) 4 Observation

13 3. Distribution of CFP causative species in Japan

14 3. Distribution of CFP causative species in Japan Gambierdiscus founding area Recent information(2008-) Before information(2006-) Our data(2008-) Old information(1980-)

15 4. Growth-physiological and genetic characteristics of Gambierdiscus toxicus Culture conditions: Water temperature: 20 or 26 Salinity: 30 or 34 Light intensity: 100 µmol photons m -2 s -1 Medium: Modified T1 medium Photoperiod: 12hL:12hD

16 4. Growth-physiological and genetic characteristics of Gambierdiscus toxicus Modified T1 medium Vitamines Compound Concentration(M) NaNO NaH 2 PO Fe-EDTA ZnSO MnCl NaMoO Trace metals CoCl CuSO EDTA-Na H 2 SeO Thiamine HCl Biotin Cyanocobalamin

17 4. Growth-physiological and genetic characteristics of Gambierdiscus toxicus Wakayama Hachijyo Culture strains from four site: Wakayama, Hachijyo Island, Akajima Island and Tahiti. Akajima Tahiti

18 4. Growth-physiological and genetic characteristics of Gambierdiscus toxicus The growth conditions of about one month after (100 µmol photns/m 2 /s) Tahiti PSU Wakayama PSU Akajima PSU Hachijyo PSU :alive ++ :growth +++:well growth

19 4. Growth-physiological and genetic characteristics of Gambierdiscus toxicus Effect of water temperature on the growth (div/day) Wakayama Hachijyo Akajima Tahiti alive alive alive -

20 4. Growth-physiological and genetic characteristics of Gambierdiscus toxicus The growth condition of about one month after ( µmol photns/m 2 /s, 34 psu) Wakayama Tahiti Hachijyo Akajima The growth condition of about one month after ( µmol photns/m 2 /s, 34 psu) Wakayama Tahiti Hachijyo Akajima

21 4. Growth-physiological and genetic characteristics of Gambierdiscus toxicus SST in winter Wakayama Hachijyo Akajima

22 4. Growth-physiological and genetic characteristics of Gambierdiscus toxicus Genetic diversity of G. toxicus culture strains in Japan Tahiti Wakayama Akajima Hachijyo A phylogenetic tree inferred from gamma weighted neighbor joining analysis using MEGA4, based on LSU rdna sequences (D8-D10). These numbers are percentage of bootstrap support values.

23 Thank you very much for your attention.

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