USDA-ARS National Plant Germplasm System Conservation of Fruit & Nut Genetic Resources
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1 USDA-ARS National Plant Germplasm System Conservation of Fruit & Nut Genetic Resources Joseph Postman Plant Pathologist & Curator National Clonal Germplasm Repository Corvallis, Oregon May 2010
2 Mission: Collect Preserve Evaluate Enhance - Distribute World Diversity of Plant Genetic Resources for Improving the Quality and Production of Economic Crops Important to U.S. and World Agriculture
3 Apple Accessions at Geneva Malus angustifolia ( 59 Accessions) Malus baccata ( 67 Accessions) Malus bhutanica ( 117 Accessions) Malus brevipes ( 2 Accessions) Malus coronaria ( 98 Accessions) Malus domestica ( 1,389 Accessions) Malus doumeri ( 2 Accessions) Malus florentina ( 4 Accessions) Malus floribunda ( 12 Accessions) Malus fusca ( 147 Accessions) Malus halliana ( 15 Accessions) Malus honanensis ( 4 Accessions) Malus hupehensis ( 185 Accessions) Malus hybrid ( 337 Accessions) Malus ioensis ( 72 Accessions) Malus kansuensis ( 45 Accessions) Malus komarovii ( 1 Accessions) Malus mandshurica ( 3 Accessions) Malus ombrophila ( 5 Accessions) Malus orientalis ( 816 Accessions) Malus orthocarpa ( 1 Accessions) Malus prattii ( 42 Accessions) Malus prunifolia ( 52 Accessions) Malus pumila ( 12 Accessions) Malus sargentii ( 23 Accessions) Malus sieversii ( 2,581 Accessions) Malus sieversii var. kirghisorum ( 35 Accessions) Malus sieversii var. turkmenorum ( 4 Accessions) Malus sikkimensis ( 14 Accessions) Malus sp. ( 41 Accessions) Malus spectabilis ( 9 Accessions) Malus sylvestris ( 70 Accessions) Malus toringo ( 122 Accessions) Malus transitoria ( 63 Accessions) Malus trilobata ( 2 Accessions) Malus tschonoskii ( 3 Accessions) Malus x adstringens ( 2 Accessions) Malus x arnoldiana ( 2 Accessions) Malus x asiatica ( 20 Accessions) Malus x astracanica ( 1 Accessions) Malus x atrosanguinea ( 2 Accessions) Malus x dawsoniana ( 2 Accessions) Malus x hartwigii ( 5 Accessions) Malus x magdeburgensis ( 2 Accessions) Malus x micromalus ( 25 Accessions) Malus x moerlandsii ( 2 Accessions) Malus x platycarpa ( 7 Accessions) Malus x purpurea ( 5 Accessions) Malus x robusta ( 13 Accessions) Malus x scheideckeri ( 2 Accessions) Malus x soulardii ( 3 Accessions) Malus x sublobata ( 4 Accessions) Malus yunnanensis ( 17 Accessions) Malus zhaojiaoensis ( 33 Accessions) Malus zumi ( 3 Accessions)
4 Grape Accessions at Davis Vitis acerifolia ( 15 Accessions) Vitis aestivalis ( 38 Accessions) Vitis aestivalis var. aestivalis ( 31 Accessions) Vitis aestivalis var. lincecumii ( 11 Accessions) Vitis amurensis ( 8 Accessions) Vitis arizonica ( 4 Accessions) Vitis betulifolia ( 1 Accessions) Vitis biformis ( 2 Accessions) Vitis blancoi ( 1 Accessions) Vitis bloodworthiana ( 1 Accessions) Vitis californica ( 9 Accessions) Vitis cinerea ( 53 Accessions) Vitis cinerea var. cinerea ( 1 Accessions) Vitis cinerea var. floridana ( 6 Accessions) Vitis cinerea var. helleri ( 20 Accessions) Vitis coignetiae ( 2 Accessions) Vitis davidii ( 1 Accessions) Vitis ficifolia ( 2 Accessions) Vitis flexuosa ( 1 Accessions) Vitis girdiana ( 4 Accessions) Vitis hybrid ( 1,051 Accessions) Vitis jacquemontii ( 10 Accessions) Vitis labrusca ( 25 Accessions) Vitis monticola ( 16 Accessions) Vitis mustangensis ( 70 Accessions) Vitis nesbittiana ( 2 Accessions) Vitis palmata ( 9 Accessions) Vitis piasezkii var. pagnuccii ( 11 Accessions) Vitis piasezkii var. piasezkii ( 2 Accessions) Vitis popenoei ( 1 Accessions) Vitis riparia ( 45 Accessions) Vitis romanetii ( 1 Accessions) Vitis rotundifolia ( 110 Accessions) Vitis rotundifolia var. munsoniana ( 16 Accessions) Vitis rupestris ( 15 Accessions) Vitis shuttleworthii ( 11 Accessions) Vitis sp. ( 172 Accessions) Vitis tiliifolia ( 7 Accessions) Vitis treleasei ( 4 Accessions) Vitis vinifera ( 184 Accessions) Vitis vinifera subsp. sylvestris ( 77 Accessions) Vitis vinifera subsp. vinifera ( 1,200 Accessions) Vitis vulpina ( 26 Accessions) Vitis wilsonae ( 1 Accessions) Vitis x andersonii ( 1 Accessions) Vitis x bourquina ( 4 Accessions) Vitis x champinii ( 16 Accessions) Vitis x doaniana ( 6 Accessions) Vitis x novae-angliae ( 1 Accessions) Vitis x slavinii ( 1 Accessions)
5 Thousands of Fruit & Nut Varieties
6 U.S. National Plant Germplasm System 441,000 Accessions 11,000 Species Germplasm Resources Information Network (GRIN)
7 History Section of Seed and Plant Introduction 1946 Research & Marketing Act (P.L. 733) Regional Plant Introduction Stations National Seed Storage Laboratory Germplasm Resources Information Network 1980s - Clonal Repositories P.L (National Genetic Resources Program) Ornamental Plant Germplasm Center
8 Active Collections Regional Plant Introduction Stations Crop Specific or Special Collections Clonal Repositories Genetic Stock Collections
9 Regional Plant Introduction Stations 1. Ames, IA (NC-7) July Pullman, WA (W-6) July Geneva, NY (NE-9) - July Griffin, GA (S-9) Nov 1949
10 Clonal Repositories 1. Geneva, NY 2. Davis, CA 3. Corvallis, OR 4. Riverside, CA 5. Miami, FL 6. Hilo, HI 7. College Station, TX 8. Mayaguez, PR 9. Washington, DC/Beltsville, MD
11 Base Collection National Center for Genetic Resources Preservation (formerly National Seed Storage Laboratory) Long Term Backup Conservation Seed Viability & Storage Research
12 National Clonal Germplasm Repositories
13 Special Collections Potato - Sturgeon Bay, WI Small Grain Aberdeen, ID Soybean Urbana, IL Cotton College Station, TX Herbaceous Ornamental Columbus, OH Arctic Plant Genetic Resources Palmer, AK (Corvallis, OR worksite) Arid Land Plant Genetic Resources Parlier, CA (Davis, CA worksite)
14 Genetic Stock Collections Maize Genetic Stock Center C.M.Rick Tomato Genetic Stock Center Wheat Genetic Stock Center Barley Genetic Stock Center Pea Genetic Stock Collection Rice Genetic Stock Center
15 National Germplasm Resources Laboratory Beltsville 1. Germplasm Resources Information Network (GRIN Database) 2. Plant Exchange Office 3. Plant Disease Research Unit
16 NCGR Corvallis Collection Summary 10,500 total plant and seed accessions (5100 field plants, 7200 screenhouse plants) Sorbus Sambucus 1% Vaccinium 1% 10% Rubus 11% Ribes 10% Clonal Accessions Actinidia 2% Corylus 9% Cydonia 1% Fragaria 18% Pyrus 26% Humulus 5% Mentha 6% Mespilus 0%
17 Repository Objectives Collect Maintain Evaluate Distribute
18 What Fruits Originated in America?
19 Showing F.C. Reimer traveling while investigating pears in northern China
20 Pyrus betulifolia Fine group of 11 trees, 4 miles east of Tien Chwang, Shantung, China - Fall
21 Hokkaido, Japan
22 Malus toringo Vaccinium oxycoccos
23 Actinidia arguta
24 Vashlovani Nature Preserve Eastern Georgia Pyrus salicifolia
25 Pyrus caucasica Caucasus Mountains, Georgia
26
27 Zebra Pear (Pysanka, Easter Egg)
28 Malus orientalis
29
30 Raspberries Blackberries Cornelian Cherries
31 Armenia Figs
32 Quince (Cydonia oblonga) Garden in Shilda Village (east Georgia)
33 Henderson Luelling's 1847 Traveling Nursery" included the following cultivars: Apples Baldwin Bellflower (Yellow Bellflower) Blue Pearmain Early Harvest Gloria Mundi Golden Russet Gravenstein King (Tompkins King) Newtown Northern Spy Rambo Red Astrachan Red Cheek Pippin (Monmouth) Rhode Island Greening Seek-no-further (Westfield Seek-no-further) Spitzenburg (Esopus Spitzenburg) White Winter Pearmain Winesap Pears Bartlett (Williams' Bon Chrétien) Clapp Favorite Early Butter (Craig) Fall Butter (White Doyenné) Pound Seckel Vicar of Winkfield (Curé) Winter Nelis Peaches Early Crawford Late Crawford Golden Cling Cherries May Duke Black Bigarreau Kentish Royal Anne (Napoleon Bigarreau) Black Tartarian Early Purple Guigne Henderson Luelling
34 Yellow Bellflower Apple (in SW Portland)
35 San Juan Island National Park (Belle Angevine or Pound Pear)
36 McDonald Forest Homestead Orchards Photos from Diana Brinn
37 Repository Objectives Collect Maintain Evaluate Distribute
38 ex situ clonal collections: Screenhouse Orchard
39 In vitro Storage
40 Cryogenic Storage
41 PGR Evaluation Virus testing Molecular identity Chemical qualities antioxidants Resistance screening
42 0.1 Fingerprinting: Blueberry PI PI PI PI a PI b PI Chandler PI PI PI CVAC 1308 PI Nahla Bassil First SSR markers in blueberry Fingerprint 72 blueberry cvs. with 28 SSRs UPGMA cluster analysis, 3 clusters PI PI PI PI PI PI PI PI PI PI PI a PI b PI PI PI PI PI PI PI PI PI PI Liberty Aurora Draper PI PI PI Jewel Star Oneal Millennia Emerald Santa Fe Southmoon PI PI Sharpblue Sapphire CVAC 1321 PI PI PI PI PI PI PI PI PI PI PI PI PI PI PI PI PI PI PI Northern Highbush Wild V. corymbosum Northern Highbush Southern Highbush Wild
43 Anthocyanin Content of different blueberry cultivars Wild type From Fla,/Ga.
44 Plant Distribution requests received 5,800 items shipped
45
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