Invaders versus endemics: alien gastropod species in ancient Lake Ohrid

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1 1 Electronic supplements for 2 Invaders versus endemics: alien gastropod species in ancient Lake Ohrid 3 Hydrobiologia 4 Christian Albrecht Kirstin Föller Catharina Clewing Torsten Hauffe Thomas Wilke 5 6 Department of Animal Ecology and Systematics, Justus Liebig University Giessen, Heinrich-Buff-Ring (IFZ), D Giessen, Germany 7 Christian.Albrecht@allzool.bio.uni-giessen.de Electronic Supplement Table 1 Newly sequenced (partial cytochrome c oxidase I) specimens of Physa acuta, Ferrissia fragilis, and Ferrissia rivularis. All haplotypes and the corresponding locality information, voucher information (University of Giessen Systematics and Biodiversity Collection UGSB) and NCBI GenBank accession numbers are provided. Superscript numbers at locality codes refer to tip lables of the phylogenetic trees. Haplotype Species Locality code Locality Latitude Longitude Specimen voucher no. 1 COI GenBank accession no. HTP 1 Physa acuta Channel Lysimachia Greece, West Greece, channel between Lake Trichonis N E UGSB KF & Lake Lysimachia UGSB KF Inflow Lysimachia Greece, West Greece, inflow to Lake Lysimachia N E UGSB KF Inflow Pamvotis Greece, Epirus, Inflow to Lake Pamvotis N E UGSB KF UGSB KF Lake Kerkini Greece, Central Macedonia, Lake Kerkini (artificial lake) N E UGSB KF Lake Lysimachia 1 Greece, West Greece, Lake Lysimachia N E UGSB KF Lake Lysimachia 2 Greece, West Greece, Lake Lysimachia N E UGSB KF Lake Ohrid 1 Macedonia, Lake Ohrid (Ohrid Bay) N E UGSB KF Lake Pamvotis 1 Greece, Epirus, Lake Pamvotis N E UGSB KF UGSB KF Lake Pamvotis 2 Greece, Epirus, Lake Pamvotis N E UGSB KF Lake Vegoritis 1 Greece, Central Macedonia, Lake Vegoritis N E UGSB KF Lake Vegoritis 2 Greece, Central Macedonia, Lake Vegoritis N E UGSB KF (peninsula opposite Arnissa) UGSB KF Lake Vegoritis 3 Greece, Central Macedonia, Lake Vegoritis N E UGSB KF (peninsula opposite Arnissa) UGSB KF Lake Vegoritis 4 Greece, West Macedonia, Lake Vegoritis N E UGSB KF River Strymonas 1 Greece, Central Macedonia, River Strymonas N E UGSB KF UGSB KF HTP 2 Physa acuta Lake Trichonis Greece, West Greece, Lake Trichonis N E UGSB KF HTP 3 Physa acuta Lake Kastraki Greece, West Greece, Lake Kastraki (artificial lake) N E UGSB KF Lake Lysimachia 2 Greece, West Greece, Lake Lysimachia N E UGSB KF HTP 4 Physa acuta Lake Dojran 1 Macedonia, Stari Dojran, Lake Dojran N E UGSB KF River Gallikos Greece, Central Macedonia, River Gallikos N E UGSB KF UGSB KF737937

2 14 HTP 5 Physa acuta Lake Dojran 2 Macedonia, Novi Dojran, Lake Dojran N E UGSB KF Lake Kerkini Greece, Central Macedonia, Lake Kerkini (artificial lake) N E UGSB KF Lake Volvi Greece, Central Macedonia, pool next to Lake Volvi N E UGSB KF UGSB KF River Strymonas 2 Greece, Central Macedonia, River Strymonas N E UGSB KF UGSB KF HTF 1 Ferrissia fragilis Lake Kalodiki Greece, Epirus, Lake Kalodiki N E UGSB KF Lake Lysimachia 3 Greece, West Greece, Lake Lysimachia N E UGSB 2534 KF Lake Mergozzo Italy, Verbano-Cusio-Ossola, Lake Mergozzo N E UGSB KF (type locality of F. wautieri) UGSB KF Lake Ohrid 2 Albania, Pogradec District, Lake Ohrid (Lin peninsula) N E UGSB KF HTF 2 Ferrissia rivularis Monocacy River USA, Maryland, Frederick Co., creek near to River Monocacy N W UGSB KF HTF 3 Ferrissia rivularis McClane Creek USA, Washington State, Thurston Co., McClane creek wetlands N W UGSB KF HTF 4 Ferrissia rivularis Newaukum River USA, Washington State, Lewis Co., North Fork River Newaukum N W UGSB KF

3 Electronic Supplement Table 2 Known occurrences of Physa acuta in natural and artificial lakes of the Balkans. The first record and the current status (latest record) are listed. Only records of live populations are considered. Presence with unknown first record is marked with a?.?? marks an unclear situation. Voucher material obtained during field work from 2003 to 2012 is deposited at the University of Giessen Systematics and Biodiversity collection (UGSB). 38 Lake First record Data source Current status Data source Amvrakia - - Not found this study Cheimaditidas? Reischütz, pers. 6/2008 this study Dojran 1985 Stanković (1985) 9/2007 this study Kastorias? Reischütz, pers. 5/2005 this study Kerkini 9/2007 this study Koronia? Reischütz, pers. -, almost this study completely dried out in 9/2007 9/2007 this study Lysimachia 8/2002 Reischütz & Mikri Prespa 7/2008 Reischütz, pers. 7/2008 Reischütz, pers. Ohrid 2009 Albrecht et al this study (2009b) Ozeros? Reischütz, pers. Not found this study Pamvotis 2002 Reischütz & 6/2013 Fehér, pers. Reischütz (2003 Paralimni 7/2004 Reischütz &?? Vardala- Theodorou et al. (2006) Petreo - - Not found this study Pikrolimni - - -, completely this study dried out in 9/2007 Prespa 8/2004; shells Reischütz, pers. - this study only Skutari 2002 Dhora (2002) 6/2012 Fehér, pers. Trichonis 8/2002 Reischütz & 9/2007 this study Vegoritis 5/2005 this study 6/2008 this study 3

4 Volvi? Reischütz, pers. 9/2007 this study Yliki 7/2004 Reischütz &?? Vardala- Theodorou et al. (2006) Zazari - - Not found this study Electronic Supplement Table 3 Known occurrences of Ferrissia fragilis in natural and artificial lakes of the Balkans. The first record and the current status (latest record) are listed. Only records of live populations are considered. Note that all published records of Ferrissia are regarded as Ferrissia fragilis, according to the results of the current study. Voucher material obtained during field work from 2003 to 2012 is deposited at the University of Giessen Systematics and Biodiversity collection (UGSB). 47 Lake First record Data source Current status Data source Amvrakia - - Not found this study Cheimaditidas - - Not found this study Dojran - - 9/2009 this study Kastorias 6/2013 Fehér, pers. 6/2013 Fehér, pers. Kerkini - - Not found this study Koronia - - -, almost this study completely dried out in 9/2007 Lysimachia 8/2002 Reischütz & 9/2007 this study Mikri Prespa - - Not found this study Ohrid this study Ozeros - - Not found this study Pamvotis 2002 Reischütz & Reischütz (2002) Paralimni 7/2004 Reischütz & 2007 Frogley & Preece (2007) 7/2004 Reischütz & 4

5 Petreo - - Not found this study Pikrolimni - - -, completely this study dried out in 9/2007 Prespa 10/2005 Albrecht et al. (2012) 2005 Albrecht et al. (2012) Skutari 2006 this study?? Glöer & Pešić (2013) Trichonis 8/2002, shells only Reischütz & 9/2007 Albrecht et al. (2009) Vegoritis 1962 Schütt (1985) 6/2008 this study Volvi - - Not found this study Yliki - Reischütz & - Reischütz & Zazari - - Not found this study

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