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1 قالوا سبحانك لاعلم لنا الا ما علمتنا انك أنت العليم الحكيم سورة البقرة اية
2 ElSeify M.A., Desouky,A.Y. & Bazh,Eman Parasitology Department, Kafr El Sheikh, Tanta University, Egypt.
3 Introduction. Fish is considered as a source of protein.. Fish production is greatly affected with parasitic infection.. Fish may act as a source of parasitic zoonoses. 4. This study was focusing on metacercarial infection of fish.
4 Aim of the work Study the incidence of different metacercariae and cysts in ten fish species. Identification of these metacercariae and cysts by excystation and animal inoculation. Collection of adult worms recovered from experimentally infected laboratory animals (rats and mice. 4Mounting and identification of the recovered worms.
5 By examination of the muscles of a total no. 669 from which 55 Oreochromis niloticus,9 Clarias lazera,9 Bagrus bayad,, 5 Schilbe mystus, 67 Mugil cephalus,, 6 Synodontis schall, 5 Mormyrus niloticus,, 4 Common carp, 40 Sardinella jussieu and 7 lates niloticus And the results revealed that ::
6 Oreochromis niloticus Clarias lazera Bagrusbayad Schilbe mystus Mugil cephalus Synodontis schall Morymorusniloticus Sardinella jussieu Common carp Lates niloticus Prevalence of infection with encysted metacercariae in different fish species
7 Concerning the percentage of infection and no. of encysted metacercariae P.G.M. the results Showed that: Fish species Total No. exam. No.+ve % of infection intensity/gm Oreochromis niloticus Clarias lazera Bagrus bayad Schilbe mystus Mugil sp Synodontis schall Morymoorus niloticus
8 Concerning the infection % & intensity among fish species the results indicated Prevalence and intensity of infection with encysted metacercaria in Oreochromis niloticus Infection in each body region of the body Mar ket Mon th To ata l no. ex am. No. +ve % of inf ec tio n Head region No. +ve (% E.M. C./gm Dorsum region No.+ ve( % E.M.C. /gm Trunk region No.+ve (% E.M.C. /gm No.+ve( % Tail region E.M.C./ gm Anal region No.+ve( % E.M.C./g m Febr uary 4 Cult ured fish Marc h April 8 7 May 8
9 .5 (00 (00.5 (00 (00 00 Sep. ( July (4.8 (4.8 ( ( June. 9( ( ( ( ( May.55 7( ( ( ( Apri l.5 ( ( ( ( ( Mar ch. 6( (87.5 (.5 5( ( Feb..8 5( (66.6 ( ( ( Jan. Nile fish Prevalence and intensity of infection with encysted metacercaria in Oreochromis niloticus (Cont.
10 6. (00 7 (00 7. (00.66 (00 00 Nov ( ( ( ( (00 9. Aug. (00 ( July ( ( ( ( May (50.66 (50.8 (50.5 6( Apr.875 8( (9.66 8( ( Mar ( ( ( (00 7 ( Jan ( (00 6 4(00.5 4( Octo. July 6.8 7( ( ( ( Apr ( (00 8 6( ( Mar. 4.8 ( ( ( (00 00 Feb. E.M.C./gm No.+ve(% E.M.C./gm No.+ve(% E.M.C./g m No.+ve(% E.M.C./g m No.+ve(% Me an E. M. C./ gm No.+ve( % Anal region Tail region Trunk region Dorsum region Head region Infection in each body region of the body %of infect ion N o. + ve To ata l no. exa m. Month Prevalence and intensity of infection with encysted metacercaria in Clarias lazera
11 Prevalence and intensity of infection with encysted metacercaria in Bagrus bayad and Schilbe mystus Fish spp. B. baya d Mon th May Jun Jan Mar Toatal no.exa m No. +v e 5 %of infec tion Head region No.+ve(% 0(76.9 6(40 Mea n E.M.C./g m. Dorsum region No.+ve(% (00 (80 Infection in each body region of the body E.M. C./gm.5. Trunk region No.+ve( % (6.66 E.M. C./gm No.+ve( % 6(46.5 0( Tail region E.M.C./gm.75 Anal region No.+ve( % 7(5.8 4(6.6 6 E.M.C./gm Jun 6.66 (00 Dec. 00 ( (00.5 S. mystus Sept. Jan (9.6 ( (8. (00. (0 0 (00.6 (9.6 (00.6 7(58. (..48 5
12 Prevalence and intensity of infection with encysted metacercaria in Mugil cephalus, Synodontis schall and Morymoorus niloticus Infection in each body region of the body Fish spp. Mont h Total no. exam. No. +ve %of infection Head region No.+ve(% E.M. C./g m Dorsum region No.+ve( % E.M. C./g m Trunk region No.+ve( % Me an E. M. C./ gm Tail region No.+v e(% Me an E. M. C./ gm Anal region No.+v e(% Mea n E.M. C./g m Feb. 4 M.ce phal us June Sept. June (6.5. 8(00.5 4( ( (. 5 S.sc hall Jan. June (45.45 (00 8(7. 7 (50. 8 (7. 7 (00.5 M.ni lotic us June Jan. Dec (80 (66.6 (50.4 0(00 (00 (00 8(80 (66. 6 ( (80 (00 (00.8
13 Concerning the identification of exycysted metacercariae it was found that:
14 Mesostephanus encysted metacercaria (X 00 (Carmine stain Hetrophyes Encysted metacercaria (X 00 (Carmine stain Cyanodiplostomum encysted metacercaria (X 40(Carmine stain Prohemistomatid encysted metacercaria (X 00 (Carmine stain
15 Haplorchid encysted metacercaria (X 00 (Fresh specimen Unidentified cyst (X40 (Fresh specimen
16 The infected fish sp. (Oreochromis niloticus & Clarias lazera were fed to the experimental animals (rats and mice and the adult worms obtained were: The obtained worms were * From Oreochromis niloticus Heterophyes heterophyes, Heterophyes aequalis, Centrocestus sp., Haplorchis pumilio, Metagonimus yokogawai
17 Adult Centrocestus sp. (X 00 (Carmine stain Adult Haplorchis pumilio (X 00 (Carmine stain
18 Adult Heterophyes aequalis (X 00 (Carmine stain Adult Metagonimus yokogawai (X 00 (Carmine stain
19 Adult Hetrophyes heterophyes (X 00 (Carmine stain
20 From Clarias lazera The obtained worms were Prohemistomum vivax, Mesostephanus appendiculatus, and Mesostephanus burmanicus.
21 Adult Mesostephanus buramnicus (X 00(Carmine stain Adult Prohemistomum vivax (X 00(Carmine stain
22 Adult Mesostephanus appendiculatus (X 00(Carmine stain
23
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