Research Article Study of Genetic Marker of Cuscuses (Marsupialia: Phalangeridae) from Maluku and Papua Based on Cytochrome b Gene Sequences
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1 OPEN ACCESS Pakistan Journal of Biological Sciences ISSN DOI: /pjbs Research Article Study of Genetic Marker of Cuscuses (Marsupialia: Phalangeridae) from Maluku and Papua Based on Cytochrome b Gene Sequences 1 Rony Marsyal Kunda, 2 Niken Satuti Nur Handayani, 3 Hery Wijayanto and 4 Rini Widayanti 1 Program Study of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 2 Department of Genetics, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 3 Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 4 Department of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
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3 Manipa Island North Seram District Jayapura Nabire Regency Buru Island Ambon Haruku South Seram District Gorom and Geser Papua Island Lakor Island Sampling sites native
4 G G E E E E E : M 982 bp 1000 bp 750 bp 500 bp 250 bp
5 Cuscus (Kariu Haruku island) whole white Cuscus (Hatu Ambon island) brown linear black Cuscus (Larike Ambon island) brown linear black Cuscus (Kariu Haruku island) brown linear black Cuscus (Allang Ambon island) whole white Cuscus (North Seram island) whole white Cuscus (Geser Seram island) whole white Cuscus (Geser Seram island) brown linear black Cuscus (Gorom Seram island) whole white Cuscus (Kariu Haruku island) brown linear black 2 95 Cuscus (Wanggar Nabire island) brown liner black 98 Cuscus (Moor Nabire island) brown liner black Cuscus (Wanggar Nabire island) gray liner black Phalanger vestitus Phalanger gymnotis 47 Cuscus (Soya Ambon island) spotted cusucs Cuscus (Nabire island) spotted cuscus 1 Cuscus (Allang Ambon island) stein cuscus Cuscus (South Seram island) spotted cuscus Cuscus (Manipa island) stein cuscus Spilocuscus maculatus Cuscus (Topo Nabire island) spotted cuscus 2 Ailurops ursinus Strigocuscus selebensis Trichosurus vulpecula Macropus parama Macropus giganteus Macropus fuliginosus 0.02
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11 ATG ACC AAC TTA CGC AAA ACC CAT TCA CTC ATC AAA ATT ATC AAC [ 45] C T [ 45] C T [ 45] C T [ 45] C T [ 45] A C [ 45] A C [ 45] A C [ 45] CAA ACC TTT ATT GAT CTC CCA ACA CCA TCA AAC ATC TCT GCC TGA [ 90] T... [ 90] T... [ 90] T... [ 90] T... [ 90] C [ 90] C [ 90] C [ 90] ACA GCT TTC TCA TCA GTA GCC CAT ATC TGT CGA GAT GTA AAC TAC [225].....C [225].....C [225].....C [225].....C [225].....C T.....C [225].....C T.....C [225].....C T.....C [225] GGC TGA CTA ATC CGA AAC TTA CAT GCC AAC GGA GCC TCA ATA TTC [270].....G C [270].....G C [270].....G C [270].....G C [270] C.... [270] C.... [270] C.... [270] GGA CAA ATA TCC TTC TGA GGA GCA ACA GTA ATC ACC AAC TTA CTA [450] T C..... [450] T C..... [450] T C..... [450] T C..... [450] C T [450] C T [450] C T [450] TCA GCC ATC CCA TAC ATC GGA ACC ACA CTA GTA GAA TGA ATC TGA [495] [495] [495] [495] [495].....T T... [495].....T T... [495].....T T... [495] ATC AAT CCA AAC TCA GAC AAA ATC CCA TTT CAC CCA TAC TAC ACA [675] G [675] G [675] G [675] G [675] A.. G [675] A.. G [675] A.. G [675] ATC AAA GAC ACC CTA GGA CTA ATT ATC ATA CTA ATT ATC CTA CTC [720] C [720] C [720] C [720] C [720] G C [720] G C [720] G C [720]
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