Why DMEK? EK: Complications and Controversies. EK: Aberrometry. Why not DMEK? Why Now? Which EK? 4/10/16. Quicker visual recovery Lower rejection rate
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1 Why DMEK? EK: Complications and Controversies Michael J. Taravella, MD Director: Cornea and Refractive Surgery University of Colorado Quicker visual recovery Lower rejection rate Less stroma? Potential for better BCVA Interface HOA 4/10/ EK: Aberrometry Corneal higher-order aberrations after Descemet s Membrane Endothelial Keratoplasty Rudolph: Ophthalmolgy 119; 3: DMEK: LESS HOA than DSAEK More SA in DSAEK group Both better than PKP 4/10/16 3 Why not DMEK? Steep learning curve High rate of damage to endothelium and loss of graft in preparation during learning curve Up to 8-10% loss (learning curve) High re-bubble rate If totally detached; unlike DSAEK; cannot re-bubble 4/10/ Why Now? Many eye banks are beginning to offer pre-prepared tissue (just like DSAEK) Eliminates one learning curve and source of loss of graft/tissue Which EK? If complex; choose DSAEK AC IOL One chamber eye Previous vitrectomy*** Tube shunt present Previous PKP (failure or rejection) 4/10/16 5 4/10/
2 Case Selection: DMEK Pseudophakic Bullous Keratopathy May need larger graft** Fuch s dystrophy Combined procedure (Cataract/EK) Dilate with Tropicamide 1% and Phenylephrine 2.5% NOT CYCLOGYL! SMALLER CAPSULORHEXIS MIOSTAT PRIOR TO INSERTION OF GRAFT 4/10/16 7 Should you block? Pros Anesthesia/Akinesia Consider Sub-tenon s Cons Cannot monitor LP during air fill Consider Topical Proparacaine 1% on surface, Xylocaine gel after prep Intracameral 1% lidocaine 2 or 4% lidocaine on surface Traction sutures to hold eye in place 4/10/ When should you perform a PI? FUGO BLADE PI Always! Especially DMEK Inferior as possible (6 o clock) Avoid bleeding FUGO BLADE Use Viscoelastic Plasma blade Controlled size and location Small size avoids diplopia 4/10/16 9 4/10/ Which gas? Air Ok for DSAEK 32 gauge retina needle; small gauge avoids back leakage 90-95% bubble if tube present; if one chamber eye Consider posterior infusion technique for one chamber eye Keeps bubble in AC Can check IOP to keep physiologic pressure tonopen on the table for glaucoma patients 4/10/16 11 SF6 Use for DMEK 95% bubble Check patient at end of procedure (Light perception) Check at Slit lamp one hour post procedure Fluid should cover PI Isoexpansive mixture 15-20% Air/SF6 mixture Decreases re-bubble rate 4/10/
3 Methods of insertion: DSAEK (Ultrathin tissue < 100 microns) Insert over a Sheet s glide Busin Glide Tan (Angiotech) Endoglide Probably not Forceps for ultrathin tissue SHEETS GLIDE ADVANTAGES: Easy to insert Easy to Deploy Must have incision about 5 to 5.5 mm?endothelial Trauma? About 12% loss from insertion 4/10/ /10/ DMEK GRAFT INSERTION Modified Glass Jones Tube IOL Cartridge Current: B&L injector Spring Removed!! Suture incision immediately SIZE OF DONOR Considerations: Larger donors = more endothelial cells Smaller donor disc = easier to deploy Guidelines W-W minus 3 DSAEK : DMEK: ** if PBK consider larger graft (up to 9.0) 4/10/ /10/ DSAEK/DMEK complications (Depth, Deployment, Dislocation) Failure to Insert properly Failure to Deploy Soft eye, AC depth Insert Upside Down Dislocation 4/10/ /10/
4 Hyperacute rejection Shallow AC 4/10/ /10/ Inversion Management Technique Inverted Graft S on stromal surface STEPS to Re-invert 1. Leave graft in eye 2. Leave air lying above graft 3. Bent needle on air to catch edge of graft 4. Pull needle back, graft will flip over around air bubble 5. Inject air through needle to re-deploy Shah VC. Reorientation of inverted endothelial graft during Descemet stripping automated endothelial keratoplasty Cornea2012 Sep;31(9): /10/ /10/16 22 Insert Upside Down (DMEK)? DMEK: Inserting Donor Tissue Confirm double scroll configuration on insertion Eidolon portable slit lamp (Price) Gentle irrigation maneuvers to flip S stamp? Yes! Helpful once deployed Confusing if on part of graft that is curled 4/10/ /10/
5 Case Video: Graft ejection 4/10/ /10/16 26 Suture immediately after insertion BUT AVOID BLEEDING!! Bubble management For any EK Make sure bubble is UNDER THE GRAFT Cannula or needle against iris Inflate slowly and monitor graft position 32 Gauge retina needle helpful in preventing back leak of air Full (90-95%) fill for 1 chamber eye, tube shunt, and DMEK Monitor LP and IOP (tonopen) for glaucoma patients 4/10/ /10/ Video Place Bubble in Right Spot! DMEK: Final Air Fill 4/10/ /10/
6 Lens management Insertion over an AC IOL AC IOL? Must decide if stable or requires replacement Advantage: less OR time if you can leave in place Disadvantage: less space for deployment, risk traumatic insertion and IOL touch If very large PI; may need to suture to make PI smaller and prevent air from going behind iris 4/10/ /10/ Advanced technique DSAEK and Glued IOL technique Consider removal of AC IOL Replace with Glued IOL Requires more OR time Scleral flaps IOL helps to separate AC from PC Less traumatic insertion of donor Consider Posterior placement of infusion Helps prevent air from going posterior 4/10/ /10/ Dislocation Diagnosis: Slit lamp exam, Visante OCT DSAEK: if partially attached (>75%) will attach eventually DMEK: if partially attached; will NOT eventually reattach Visante OCT: complete graft dislocation 62 year old Post-op day 1 4/10/ /10/
7 Timing to re-bubble DSAEK: not urgent; easier if graft partially attached DMEK: as soon as possible Technique Minor OR: prep eye and lids Lid speculum 32 gauge needle At slit lamp? Re-bubble: How many times? Primary graft failure? Traumatic insertion? Endothelial viability? 3 strikes your out 1 or 2 times if endothelial viability questioned 4/10/ /10/ ASCRS 2016 Film 116 Ergonomic Technique for Rebubbling DMEK Grafts at the Slitlamp Using IV Extension Tubing Producer: Mark Terry Donor Issues Inspect Donor Review Donor Information Sheet How large is stromal bed? Determines maximum trephine size Irregular cut? Usually noted on prep sheet from eye bank 4/10/ /10/16 40 Rejection in DSAEK/DMEK DSAEK: incidence uncertain 2 years DMEK: < 1% (Price, et 2 years Rejection in DSAEK/DMEK Less inflammation and AC reaction Unexplained corneal edema with minimal KP Harder to recognize Long term steroid use for DMEK/DSAEK? Consider FML BID Restasis? 4/10/ /10/
8 Topical Steroids in EK Long term steroid use for DMEK/DSAEK? Prednisolone Acetate 1% Taper over 3 months Lotemax: if steroid responder Consider FML BID Restasis? 4/10/ /10/ Michael.taravella@ucdenver.edu 4/10/
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