Hawaii Ocean Time-series HOT-200 General Cruise Plan Created on 2/21/2008 3:25 PM

Transcription

1 HOT-200

2 Hawaii Ocean Time-series HOT-200 General Cruise Plan Created on 2/21/2008 3:25 PM Vessel: R/V Kilo Moana, University of Hawaii Master of the Vessel: Captain Brian Wehmeyer Chief Scientist: Eric Grabowski, University of Hawaii OTG Marine Technicians: Kuhio Vellalos and Elly Speicher Kilo Moana phone number: , cell # Marine Center phone number: Loading: February 21, 0900 Departure: February 22, 0900 Arrival: February 26, SCIENTIFIC OBJECTIVES The objective of the cruise is to maintain a collection of hydrographic and biogeochemical data at the Hawaii Ocean Time-series (HOT) stations. Four stations will be occupied during the cruise, in the following order: 1) Station 1, referred to as Station Kahe, is located at 21 o 20.6'N, 158 o 16.4'W and will be occupied on the first day of the cruise for about 2 hours. 2) Station 2, referred to as Station ALOHA is defined as a circle with a 6 nautical mile radius centered at 22 o 45'N, 158 o W. This is the main HOT station and will be occupied during the 2 nd, 3 rd, and 4 th days of the cruise. 3) Station 52, is the site of the WHOTS Mooring, located at 22 o 'N, 157 o W will be occupied on the 4 th day of the cruise for about one hour. 4) Station 6, referred to as Station Kaena, is located off Kaena Point at 21 o 50.8'N, 158 o 21.8'W will be occupied on the 4 th day of the cruise for about 2 hours. 1.1 SCIENTIFIC OPERATIONS Station Activities Kahe (sta. 1) Weight Cast, PRR cast, CTD cast (1000 m), Go-Flo cast (~ 20m) ALOHA (sta. 2) Sediment traps, gas array, net tows, CTD operations, primary productivity measurements, AC9/FRRf, misc. experiments. WHOTS mooring station One CTD cast (200 m). (Sta. 52) Kaena (sta. 6) CTD cast (2400 m) Underway/continuous ADCP, thermosalinograph, fluorometry, meteorology Final V.3 1

3 2.0. SCIENCE PERSONNEL Participant Title Affiliation/HOT Group Eric Grabowski Chief Scientist Res. Assoc. UH/BEACH Susan Curless Research Associate UH/BEACH Lance Fujieki Computer Specialist UH/BEACH Adriana Harlan Research Associate UH/BEACH Karin Björkman Research Specialist UH/BEACH Binglin Li Graduate Student UH/BEACH Brett Updyke Technician UH/BEACH Blake Watkins Marine Engineer UH/BEACH Sam Wilson Scientist UH/CMORE Tara Clemente Research Associate UH/BEACH Jay Wheeler Research Associate UH/BEACH Solange Duhamel Postdoc UH/BEACH Paul Lethaby Research Associate UH/PO Christin Shacat Research Associate UH/PO Fernando Santiago-Mandujano Research Associate UH/PO Jefrey Snyder Marine Technician UH/PO Sarah Yasui Volunteer UH/PO Nicolas Petrochilos Volunteer UH/PO John Fitzpatrick Volunteer UH/PO Kathryn Stanaway Volunteer UH/BEACH Janice Jones Technician UCSB Sara Yeo Technician UH/HIMB Gordon Walker Teacher UH/CMORE Doug Weidman Teacher UH/CMORE Dan Hendricks Teacher UH/CMORE Kate Achilles CMORE Educator UH/CMORE Kuhio Vellalos Marine Technician OTG Elly Speicher Marine Technician OTG 3.0. SUMMARY SCHEDULE 14 February Pre-cruise meeting, MSB 307, 1030hrs 21 February Ship loading starting at 0900 hrs 22 February Depart from Snug harbor at 0900 hrs. Science personnel on-board by February Station 1 Kahe Pt. operations February Station ALOHA operations. Station 52 and Kaena CTD casts. 26 February Arrive back to Snug harbor. ETA 0800 hrs, full offload 4.0. OPERATIONAL PLANS Final V.3 2

4 4.1. Station Kahe (21 o 20.6'N, 158 o 16.4'W) A 1000 lb. weight-test cast, one CTD cast to 1000 m, one Go-Flo cast to approx. 20m and a PRR cast (Sect ) will be conducted at this location in the afternoon of February 22nd. The CTD winch and crane will be required for these operations. The Go-Flow cast will require the use of the capstan and A-frame. After the operations are satisfactorily completed, the ship shall proceed to Station ALOHA Station ALOHA (22 o 45'N, 158 o W with 6 nm radius) Upon arrival to Station ALOHA, the sediment traps will be deployed. Afterwards, one 350-m and one 1000-m casts will be conducted before deploying the Primary Productivity array (Sect ). These operations will be followed by a near-bottom CTD cast Sediment trap array deployment Upon arrival to Station ALOHA, the floating sediment traps will be deployed at a location within Station ALOHA, which will be determined by local current conditions to be determined enroute to ALOHA. The array will be deployed from the stern using the A-frame and the Sea-Mac winch. Power requirement for the winch is 440 VAC, three phase at 10 amps. After deployment we request that the Bridge verify that the radio transmitters are functioning and directionally correct. The array will drift for about 53 hours before recovery. The array is equipped with 2 ARGOS satellite transmitters (platform #s 01833, 03028, 60482, 60484), 2 strobe lights, and 2 radio transmitters (channel 72, MHz). Daily positions of the array shall be transmitted by directly to the ship (argosfix@km.soest.hawaii.edu, password: argosfix), therefore the ship will not need to keep within site of the array until the time of the recovery. Assistance from the bridge is requested in plotting the drift track of the array. We request the use of the ship's radio direction finder for locating the array before recovery. After deployment of the sediment trap array, the ship shall return to the center of Station ALOHA to continue with CTD cast operations Primary production experiment Samples for the primary productivity experiment will be collected from the rosette. Before dawn (sunrise 0657 hrs on February 23), a free drifting incubation array will be deployed from the stern. We request the use of the A-frame for this operation and will also use the Sea-Mac winch. The array is equipped with one ARGOS satellite transmitter (platform # 60481, ing positions to argosfix@km.soest.hawaii.edu, password: argosfix), strobe lights and a radio transmitter (channel 68, MHz). The ship shall keep within site of the array while performing CTD operations for the last 6 hours of the approximately 12-hour time the array will be in the water. The array will be recovered just at sunset (1833 hrs). CTD operations shall continue after recovery. All radioactive waste generated by the experiment shall be returned to the University of Hawaii. Only qualified personnel shall handle radioactive material Water column measurements Final V.3 3

5 Vertical profiles of temperature, conductivity and dissolved oxygen will be made with an instrument package consisting of a Sea-Bird CTD attached to a 24-place rosette with 12 liter sampling bottles. We need the ship s CTD winch and crane for this operation. Water samples for biogeochemical measurements will also be collected on each cast. The cast after the deployment of the primary productivity array shall be made to the near bottom (approximately 4740 m). Following this cast, a series of 1000-m casts shall be made continuously every 3 hours for a 36-hour period, ending with a second near-bottom cast. It is highly desired that this burst sampling be done without interruption and we request the ship to maintain position within the study area for that period of time, and repositioning to the center of the Station before each cast whenever possible. Whenever pumping of the ship s tanks is needed, it must be conducted outside the circle that defines station ALOHA (Sect. 1.0). To avoid disruptions in the schedule, this operation should be coordinated with the chief scientist or the watch leaders (Fernando Santiago- Mandujano, Paul Lethaby) Gas Array deployment A second free drifting incubation array will be deployed the third day of the cruise at ALOHA station. Samples for the gas array will be collected from cast 9. We request the use of the A-frame for the gas array deployment, and will also use the Sea-Mac winch. The array is equipped with one ARGOS satellite transmitter (platform # 08500, ing positions to argosfix@km.soest.hawaii.edu, password: argosfix), a strobe light and a radio transmitter (channel 68, MHz). The ship will not need to keep within sight of the array until the time of the recovery, approximately 24 hours after its deployment. CTD operations shall continue after the recovery Zoo net tows A plankton net will be deployed from the stern and shall be towed for half-hour periods. Half-hour periods are scheduled at around noon and two consecutive half-hour periods at midnight on the second, third, and fourth days (see schedule) with a total of eight available slots to accommodate cancellations due to sea state or other unforeseen problems. The A-frame and capstan will be needed for this operation. B. Watkins will be in charge of these operations Kate Achilles net tows The hand held surface net tows are deployed off the stern for about minutes. The ships deck equipment is not needed for this operation. We request that the ship remain stationary during these tows. Kate Achillies will be in charge of these tows Profiling Reflectance Radiometer (PRR). Around noon on each day a profiling reflectance radiometer will be deployed from the main deck using the A-frame. The instrument is hand-lowered and retrieved with assistance from the winch AC9/FRRf Final V.3 4

6 The Wet Labs AC9 is an optical instrument that measures water column spectral absorption and attenuation at nine wavelengths. The AC9 package also includes a Fast Repetition Rate Fluorometer (FRRf), and a Sea-Bird Seacat with temperature, conductivity, fluorometer, and pressure sensors. The package will be deployed to a target depth of 250 m at a constant speed of 10 m/min during the downcast and upcast. The A-frame and capstan will be needed for this operation. 4.3 Floating sediment trap recovery In the morning of February 25, after the AC9/FRRf cast has been completed, we shall transit for the recovery of the floating sediment trap array. The A-frame and the Sea-Mac winch will be needed to retrieve the sediment trap array. After the array is recovered, the ship shall transit to recover the Gas Array. After the array is recovered, the ship shall transit to Station ALOHA to conduct one PRR cast, and two AC9/FRRf casts, after which the ship shall transit to Station 52 to conduct one CTD cast. 4.4 WHOTS Mooring (Station 52) One 200-m CTD yo-yo cast will be conducted near the WHOTS mooring on February 25. This cast should be conducted downwind, downcurrent, and at about 200 m from the mooring. The nominal position of the mooring is 22 o 'N, 157 o W. After the cast is completed the ship shall transit to Station Kaena. 4.5 Station Kaena (21 o 50.8'N, 158 o 21.8'W) A near-bottom CTD cast (~2500 m) will be conducted at this location in the evening of February 25, after which the ship shall return to Snug harbor. 4.6 Acoustic Doppler Current Profiler The ship s acoustic Doppler current profiler (ADCP) will be in operation during the duration of the cruise. The OTG electronics technician will be in charge of the ADCP system. 4.7 Thermosalinograph and Fluorometer The ship s thermosalinograph and fluorometer sampling the uncontaminated seawater supply system will be in operation during the duration of the cruise while the ship is outside of Snug harbor. Salinity samples to calibrate the thermosalinograph will be taken from the intake hose at 4-hour intervals throughout the duration of the cruise by the science personnel. The OTG electronics technician will be in charge of the thermosalinograph and fluorometer operations. 4.8 Magnetometer The ship s magnetometer will be deployed two times during the cruise. The first deployment will be during the transit to Station ALOHA on February 22 and the second will be during the transit to Station Kaena on February, 25. It will take roughly 10 minutes to deploy and recover this instrument. The magnetometer needs to be deployed before the ship reaches cruising speed. OTG personnel will be incahrge of this operation. They will notify the watch leaders when the magnetometer is deployed and recovered. Final V.3 5

7 5.0 EQUIPMENT 5.1 The HOT science party shall be bringing the following 1. Seabird CTD system, all sensors, deck boxes and computer CTD acquisition systems 2. Rosette and l water sampling bottles, all spare parts 3. Two laboratory vans with assorted equipment for radioisotope and general use 4. Distilled, deionized water and all required chemicals and isotopes 5. Storage van with assorted equipment (main deck) 6. Large vacuum waste container 7. Liquid nitrogen dewer 8. Drifting sediment trap array with strobe lights, satellite and radio transmitters, floats, weights 9. Kevlar line, polypropylene line 10. Sediment traps and crosses 11. Drifting primary production array and gas array with light and radio transmitter, floats, weights, polypro. Line, spare buoy, etc. 12. PRR, AC-9/FRRf and other optical measuring instruments. 13. Oxygen titration system 14. Plankton nets and towing lines 15. Desktop and laptop personal computers 16. Assorted tools 17. All required sampling bottles. 18. Deck incubation system 19. Pertinent MSDS 5.2. We will need the use of the following ship's equipment: 1. A-frame 2. A-frame block assembly 3. Appleton crane and winch with conducting wire for CTD 4. Electric power for winches (440 VAC, 3 phase, 60 Amp breaker) and vans (208 VAC single phase at 60 amps for labvan, 110 VAC 10 amps for equipment van) 5. Radio direction finder 6. Space on the main deck for one storage van 7. Space on upper deck for two lab vans port side, one OTG rad van starboard side 8. Space on upper deck for incubators 9. Hand-held VHF transceivers 10. Precision depth recorder 11. Shackles, sheaves, hooks and lines 12. Shipboard Acoustic Doppler Current Profiler 13. Thermosalinograph, pco2 system, and Fluorometer 14. Copy machine 15. Grappling hooks and line 16. Navlink2 PC or equivalent Final V.3 6

8 17. Running fresh water and seawater, hoses 18. Electronic mail system 19. GPS system 20. Uncontaminated seawater supply 21. Small capstan (~ 10 m/min) 22. Underway/on-station data acquisition system for meteorological instruments, ADCP, thermosalinograph, fluorometer, pco2 23. OTG s 24-place rosette, and l water sampling bottles (to be used as spare) 24. Pinger (to be used as spare) lb weight. 26. Remote CTD decibar pressure display in the winch operator cabin. 27. Large Sea-Mac winch (Mod EHS). 60 Amp Hubbel plug/connector (440 VAC, 3 phase, 60 Amp breaker) 28. OTG rad van Final V.3 7

9 Ship: R/V KILO MOANA HOT 200 CTD CASTS Feb. 22 Feb Cast Samples #Bottles Kahe Pt. s1c m O 2, Temp, DIC/Alk, Nuts, Chl a, LLN, LLPO 4, 24 DOC, FCM, KA(sample at 750,25,5),Salts Station ALOHA s2c1 350 m CMORE(5@25, 5@45, 5@75) KB(1@350,200)(7@30) 24 s2c m Primary Production, Salts, SW (2@10),SD(pb PP depths) MB(pb on all depths),kb(1@1000) 24 s2c m (PO-1) O 2, Temp, DOC, DIC/Alk, Nuts, KA(pb oxygen),salts 24 s2c m (PO-2) O 2, Temp, Nuts, DIC/Alk, Quay, DOC, SD(pb nut depths), Salts 24 s2c m PC/PN, SW(1@5,25,45,75,100,125,150,175), 22 Salts s2c m PPO4, CMORE(5@125, 5@200), Salts 24 s2c m (BEACH) O 2, Temp, DIC/Alk, Nuts, LLN, LLP,DOC, Keeling, Quay, SD(pb nut depths), Salts 23 s2c m PUR, CMORE(5@1000, 5@770, 5@500), Salts 24 s2c m Gas Array (2@125,100) (3@5,25,45,75) 24 MB (1@175,150,125,100,75,45,25,5) s2c m Open, MR(1000,800,600,400,200,175,150,125,100,75,45,10), BL(1@300)(6@45), Salts 21 s2c m PSi, MC(5,25,45,75,100,125,150,175), Salts 24 SW(1@5,25,45,75,100,125),SW(pbMC,150,175),KA(pbMC) s2c m MIT, Salts 16 s2c m ATP, Quay(100,125,150,200,300), MC(200,300,500,770), SD(pb all ATP depths), Salts 20 s2c m PE, Salts, MC(5,25,45,75,100,125,150,175),SW(pb MC) Quay (5,25,45,75), Salts 24 s2c m HPLC, Chl a, Slides, Salts 22 s2c m (PO-3) Oxygen, Salts, MC(1000,2000,3000,4000), 24 MR(4000,3000,2000,1000,800,600,400,175,125,100,75,10) WHOTS Mooring S52c1 200 m JE(8@15),BL(1@5,25,45,75,100,125,150), Salts 18 Kaena S6c m Chl, Salts 13 Final V.3 8

10 SHIP R/V KILO MOANA HOT 200 Date Feb Feb. 26, 2008 TIME Fri. 2/22 Sat. 2/23 Sun. 2/24 Mon. 2/25 Tue. 2/ S2C Net Tow S2C2 PP 0200 S2C9 Gas 0300 AC9/FRRF 0400 Deploy PP Array Deploy Gas Array Transit sed traps 0500 S2C3 PO-1 S2C10 Open 0600 Recover traps Transit gas array S2C11 PSi Recover gas array Arrive Snug 0900 Depart Snug Transit ALOHA 1000 Net Tow Net Tow 1100 Arrive Kahe (11:30) S2C4 PO-2 S2C12 MIT PRR Weight cast (Begin 36 hr) AC9/FRRF 1200 Net Tow PRR PRR AC9/FRRF AC9/FRRF 1300 S1C1 KA hand net tow Net Tow Transit St Go-Flo cast S2C5 PC/PN S2C13 ATP S52C Transit ALOHA deploy magnetometer 1600 Transit St. Kaena deploy magnetometer 1700 S2C6 PPO4 S2C14 PE Recover PP array 2000 S2C7 BEACH S2C15 HPLC Recover magnet 2100 S6C Recover magnet 2300 Arrive ALOHA Deploy sed traps Net Tow Net Tow S2C8 PUR S2C16 PO-3 (end 36 hours) Transit Snug February 23: Sunrise 0657 Sunset 1833 Final V.3 9

11 6.0 HOT-200 Watch Schedule Adriana Harlan - Water Boss Lance Fujieki Watch Leader - Tag Eric Grabowski Chief Scientist -alt water boss Kathryn Stanaway- Jefrey Snyder Console Nicolas Petrochilos- alttag Christin Shacat Jay Wheeler - Tag Susan Curless Water Boss Karin Björkman Tag Paul Lethaby - Watch Leader - Console John Fitzpatrick- alttag Sarah Yasui Tara Clemente - Tag Brett Updyke At Large Blake Watkins Sara Yeo Janice Jones Binglin Li Sam Wilson Gordon Walker Doug Weidman Dan Hendricks Kate Achilles Solange Duhamel OTG Kuhio Vellalos Elly Speicher Final V.3 10

12 Hawaiian Ocean Time-Series HOT-200 KAHE Station Data Sheet Station # 1 Date: (HST) Cast # 1 Time: 13:00 (HST) Operator(s): AH, LF, EG, JW Rosette Position Desired Depth Oxygen Sample Temp. Nuts DIC/ Alk ph DOC LLN/ LLP Chl a ,5, A-B A-B A-B A-B A-B A-B , A-B 18A-B, A-B A-B A-B 22A-B A-B 24 5 QC 24.4 FCM Notes: KA(pb on 750, and take 3L from 5 and 25) FCM had 40ul of PFA +2ml of sample per tube. Samples sat on bench till 17:15 and were then flash frozen.

13 HOT- 200 OPEN CAST Data Sheet Station # 2 Date: (HST) Cast # 1 Time: 0000 (HST) Operator(s): SC, KB, TC, JW, BU Rosette Position Desired Depth KB CMORE X X 3 75 X 4 75 X 5 75 X 6 75 X 7 75 X 8 45 X 9 45 X X X X X X X X X X X X X X X X Notes:

14 HOT-200 Primary Production Data Sheet Station # 2 Date: (HST) Cast # 2 Time: 0215 (HST) Operator(s): SC, KB, TC, BU, JW Rosette Position Desired Depth Light Bottle Chl a FCM KB MB x 2 Sal min A-B 3A-B X A-B 4A-B X X SD X SD X SD X SD X SD SW SW X SD Notes: MB and SD will piggy back on all the PP depths. PLEASE SAMPLE PP FIRST and CONSERVE the WATER.

15 HOT-200 WOCE Deep Data Sheet Station # 2 Date: 2/23/08 (HST) Cast # 3 Time: 0620 (HST) Operator(s): AH,LF,EG Rosette Position Desired Oxygen Sample Nutrient Depth Temp Refridg. Si DOC DIC/ Alk ,31, 3.6 3A-B 3A-B 3A-B 3A-B 3A-B-C ,36, 3.8 6A-B 6A-B 6A-B , A-B 11A-B 11A-B ,50, A-B 16A-B 16A-B Notes: KA(pb on all depths for oxygen) ph

16 HOT-200 PO Shallow Data Sheet Station # 2 Date: 2/23/08 (HST) Cast # 4 Time: 1115 (HST) Operator(s): AH,LF,EG,JW Rosette Position Desired Depth Oxygen ,61, 62 Sample Temp. Nutrient Refridg. Si DIC/ Alk ph DOC SD 6.3 1A-B 1A-B X X ,68, A-B 11A-B ,77, X A-B 17A-B ,84, A-B X X X Notes: SD(pb nut depths)

17 HOT- 200 PC/PN Data Sheet Station # 2 Date: 2/23/08 (HST) Cast # 5 Time: 14:00 (HST) Operator(s): SC, TC, KB Pre-screen mesh size: 202 um Blank #'s B1 B2 B3 Rosette Position Desired Depth Carboy # Total Volume Sample # SW Sal min X X X ,8 4,4 12A-B X X X X ,13 4,4 20A-B X X X JJ Notes: Niskin #3 spigot came off during recovery all water lost.

18 HOT- 200 Particulate Phosphorus Data Sheet Station # 2 Date: (HST) Cast # 6 Time: 17:10 (HST) Operator(s): SC, TC, KB Pre-screen mesh size: 202 um Blank #'s B1 B2 B3 Rosette Position Desired Depth Carboy # Total Volume Sample # CMORE Sal min X X X X X ,8 4,4 14A-B X X X X X ,13 4,4 23A-B Notes:

19 HOT-200 BEACH Shallow Data Sheet Station # 2 Date: (HST) Cast # 7 Time: 2000 (HST) Operator(s): SC, TC, KB Rosette Position Desired Depth Oxygen Sample Temp. Nutrient Refridg. Si DOC LLN LLP/ LLSi O 2 min Sal min A-B A-B ,102, A-B A-B A-B Notes: SD(pb nut depths)

20 HOT-200 BEACH Carbon Data Sheet Station # 2 Date: (HST) Cast # 7 Time: 2000 (HST) Operator(s): SC, TC, KB Rosette Position Desired Depth DIC/ ALK ph Quay DIC Keeling DIC O 2 min 3 Sal min A-B A-B 7, A-B 24 Notes: Keeling 20a 21:49, 20b 21:51 Keeling 23a 21:53, 23b 21:55 **bubbles stuck on Keeling bottle 23a and 200ul of HgCl2 added to Keeling bottles.

21 HOT-200 PUR Data Sheet Station # 2 Date: (HST) Cast # 8 Time: 2300 (HST) Operator(s): SC, TC, BU, KB Rosette Position Desired Depth Carboy # Total Volume PUR CMORE X X X X X X X X X X 11 Sal min X X X X X ,8 4,4 19A-B ,12 4,4 22A-B Notes: SAM pb on niskin 20 and 21

22 HOT- 200 Gas Array Experiment Data Sheet Station # 2 Date: (HST) Cast # 9 Time: 0200 (HST) Operator(s): SC, TC, KB Rosette Position Desired Depth 15N2 MB QUAY X X X X X X X X X 9 75 X X X X X X X X X X X X X X X 21 5 X 22 5 X 23 5 X 24 5 X X Notes:

23 HOT- 200 OPEN CAST Data Sheet Station # 2 Date: 2/24/08 (HST) Cast # 10 Time: (HST) Operator(s): AH,LF,JW,EG Rosette Position Desired Depth MR BL X X X 5 Sal min X X X X X X X X X X X X X X X X X X 24 Notes:

24 HOT- 200 Particulate Silica Data Sheet Station # 2 Date: 2/24/08 (HST) Cast # 11 Time: 0800 (HST) Operator(s): AH,LF,EG,JW Pre-screen mesh size: none Blank # B1,B2,B3 Rosette Position Desired Depth Carboy # Total Volume Sample # MC SW KA Sal min X x X X x X ,10 4,4 7A-B X X x X X x X X x X X x ,15 4,4 19A-B X X x X 24 5 x X Notes: SW(pb) MC 150,175; KA (pb)mc all depths Filter 7A for PSi was dropped on the floor.

25 HOT- 200 MIT Data Sheet Station # 2 Date: 2/24/08 (HST) Cast # 12 Time: 1100 (HST) Operator(s): Ah,lf,eg,jw Rosette Position Desired Depth MIT SW x X X X X 6 Sal Min x X X X Notes:

26 HOT- 200 ATP Data Sheet Station # 2 Date: 2/24/08 (HST) Cast # 13 Time: 1400 (HST) Operator(s): SC, KB,TC Pre-screen mesh size: 202um Blank #'s 28, 29, 30 Rosette Position Desired Depth ATP Tube #'s Volume Filtered Carboy # QUAY X 3 Sal min X x X X x X X x X x X x X x x x x MC Notes: SD(pb ATP depths)

27 HOT-200 Phycoerythrin Data Sheet Station # 2 Date: (HST) Cast # 14 Time: 1700 (HST) Operator(s): SC, KB, TC Pre-screen mesh size: None Rosette Position Desired Depth Carboy # Total Volume 10um 5um.4um MC qu ay Sal min X X X X X X X X X X X X Blanks Notes: SW (pb MC) Carboys 9, 10, and 12 aborted at 21:53 CB 9 = 2.5L left, CB 10 = 3L left, CB 12 = 2L left

28 HOT-200 HPLC & Chl a. Bottle Data Sheet Station # 2 Date: (HST) Cast # 15 Time: 2000 (HST) Operator(s): SC, KB, TC, BU Rosette Position Desired Depth Carboy # Total Volume HPLC Chl a. SLIDES Sal min BW BW A-B ,9 4,4 8A-B BW BW BW A-B ,16 4,4 17A-B BW BW BW Notes: DO NOT PRE-SCREEN,

29 HOT-200 WOCE Deep 2 Data Sheet Station # 2 Date: (HST) Cast # 16 Time: 2300 (HST) Operator(s): SC, KB, TC, BU Rosette Position Desired Depth Oxygen Sample Temp. MC MR JW x X X X X X X X X 13 O2 min X X 16 Sal min X X X X 21 O2 max X X Notes:

30 HOT- 200 STATION 2 Data Sheet Station # 2 Date: 2/25/08 (HST) Cast # 17 Time: 1000 (HST) Operator(s): EG,LF,JW,AH Rosette Position Desired Depth JE BL X X X X X X X X 9 75 X X X X X X X X X X X X X X 23 5 X 24 5 X Notes: JE water is pre-filtered w/202um, fill up 4-20L carboys and then put in incubator

31 HOT-200 KAENA Data Sheet Station # 6 Date: (HST) Cast # 1 Time: 2054 (HST) Operator(s): SC, KB, TC Rosette Position Desired Depth Chl a Notes:

32 Data Sheet for Sediment Trap Volumes Cruise #: 200 Analyst: AH, JW Directions: 1) Mark the traps with 2 lines a) Line #1 is at the interface b) Line #2 is 2" (5 cm) above the interface 2) Siphon off the top of the trap to Line #2-2" above the interface 3) Measure the distance from the bottom of the trap to Line #2 2" above the interface and record the result in this table. Trap Name Depth (m) Height (cm) at Line #2 (Top Line) Volume (L) at top line = (Height in cm x 0.038) A B C D E F G H I J K L

33 HOT-200 Sediment Trap Data Sheet Type of traps: PIT Date: Operator(s): Wind: Calm Position in: W N Sea State: 3-4ft Time in: 150 m X LIVE Notes: Traps in water 23:12 Array released 23:24 (HST) X DEAD 300 m 500 m Operator(s): AH,BW,JW,LF Date: 2/25/08 Position out: N W Wind: Overall sea state: Sea state: Time out: 150 m 0635 Notes: (HST) 300 m 500 m General processing procedure: 1) Cap traps immediately upon retrieval. 2) Mark interface and 2 inches above. 3) Remove baffles. 4) Aspirate overlying sea water to 2 inch interface mark. 5) Prescreen all traps at a given depth through a single screen and save screened material in trap blank solution. 6) Pour the contents of 9 individual screened traps into separate filtration bottles (do not combine trap solutions) marked A-I. 7) Pour the contents of 3 screened traps back into respective traps (do not combine trap solutions) for mass flux analysis (J,K,L). 8) Filter 3 two liter and 3 one liter time zero blanks for PC/PN & PPO 4 respectively. 9) Filter 6 individual traps for PC/PN (A-F) and 3 for PPO 4 (G-I).

34 HOT-200 In Situ Gas Array Data Sheet Operator(s): LF,EG,BW,JW,AH Date in 2/24/08 Time in 0435 Position in: N W Notes: Operator(s): AH,LF,BW,JW,EG Date out: 2/25/08 Time out: Position out: N W Notes:

35 HOT- 200 In Situ Primary Production Data Sheet Operator(s): BW,EG,LF,BU,AH,SC,TC,KB Date in: 2/23/08 Time in: 0430 (HST) Date out: Time out: 19:15 (HST) Incubation Depth Insertion Depth Owner Position in: N W Position out: N W Average weather condition during incubation: 2/8 Sky Cover Average sea state during incubation: 3-4ft Notes: Begin Inoculation Filtration time End Inoculation

36 HOT-200: Chief Scientist Report Chief Scientist: Eric Grabowski HOT-200 Chief Scientist's Cruise Report R/V Kilo Moana February 22-February 26, 2008 Cruise ID: KM0802 Departed: February 22, 2008 at 0900 (HST) Returned: February 26, 2008 at 0800 (HST) Vessel: R/V Kilo Moana Operator: University of Hawaii Master of the Vessel: Captain Brian Wehmeyer Chief Scientist: Eric Grabowski OTG Electronics/Deck Operations Technicians: Kuhio Vellalos and Elly Speicher 1. SCIENTIFIC OBJECTIVES The objective of the cruise is to maintain a collection of hydrographic and biogeochemical data at the Hawaii Ocean Time-series (HOT) stations. Four stations will be occupied during the cruise, in the following order: 1) Station 1, referred to as Station Kahe, is located at 'N, 'W and will be occupied on the first day of the cruise for about 2 hours. 2) Station 2, referred to as Station ALOHA (A Long Term Oligotrophic Habitat Assessment) is defined as a circle with a 6 nautical mile radius centered at 22 45'N, 158 W. This is the main HOT station and will be occupied during the 2 nd, 3 rd, and 4 th days of the cruise. 3) Station 52, is the site of the WHOTS Mooring, located at N, W and will be occupied on the 4 th day of the cruise for about 1 hour. 4) Station 6, referred to as Station Kaena, is located off Kaena Point at 'N, 'W will be occupied on the 4 th day of the cruise for about 3 hours. Upon arrival to Station Kahe a 1,300 lb. weight-test cast to 500 m, one CTD cast to 1000 m, and a PRR cast was to be conducted at this location in the afternoon of February 22nd. The single CTD cast was to be conducted to collect continuous profiles of various physical and chemical parameters. Water samples were to be collected at discrete depths for biogeochemical measurements. After these operations were satisfactorily completed, the ship was to proceed to Station ALOHA. Upon arrival at Station ALOHA, the free-drifting sediment trap array was to be deployed. The sediment trap array was to stay in the water for about 52 hours. This was to be followed by one shallow CTD cast to 350 m and one 1000 m CTD cast to collect water for the primary productivity array. After this, the free-drifting primary productivity array was to be deployed for 12 hours. A full-depth CTD cast was to be conducted after the deployment of the primary prodcution array, followed by 1000-m CTD casts at strict 3

37 hour intervals for at least 36 hours for continuous and discrete data collection, ending with another full-depth CTD cast on February 24 th. Another free-drifting array (gas array) was to be deployed for 24 hours for incubation experiments on February 24 th. The gas array was to be recovered at 0800 on February 25 th. A plankton net was to be towed near noon and midnight for 30-min intervals on February 23 rd and 24 th at Station ALOHA. A Profiling Reflectance Radiometer (PRR) was to be deployed for half-hour periods near noon time on February 24 th and 25 th. A package including a Wet Labs AC9, a Chelsea Fast Repetition Rate Fluorometer (FRRf), and a SeaBird Seacat was to be used to profile the upper 200 m at Station ALOHA around noon time on February 24 th and 25 th and in the early morning on February 25 th. After CTD work at Station ALOHA was accomplished, the ship was to transit to recover the floating sediment trap array and the gas array on February 25 th. After recovering the arrays, the ship was to transit back to Station ALOHA to conduct light casts (PRR, AC9/FRRf). After operations at Station ALOHA ended, the ship was to transit to Station 52 to conduct a one-hour 200-m CTD yo-yo cast, after which the ship was to transit to Station Kaena. A near-bottom CTD cast (~2500 m) was to be conducted at Station 6 including salinity and chlorophyll samples for calibration, after which the ship was to transit to snug harbor. The following instruments were to collect data throughout the cruise: shipboard ADCP, thermosalinograph, underway fluorometer, two anemometers, and the pco2 system.

38 2. SCIENCE PERSONNEL BEACH group: Cruise Participant Title Affiliation Eric Grabowski Chief Scientist Res. Assoc. UH/BEACH Susan Curless Research Associate UH/BEACH Lance Fujieki Computer Specialist UH/BEACH Adriana Harlan Research Associate UH/BEACH Karin Björkman Research Specialist UH/BEACH Binglin Li Graduate Student UH/BEACH Brett Updyke Technician UH/BEACH Blake Watkins Marine Engineer UH/BEACH Sam Wilson Scientist UH/CMORE Tara Clemente Research Associate UH/BEACH Jay Wheeler Research Associate UH/BEACH Solange Duhamel Postdoc UH/BEACH Kathryn Stanaway Volunteer UH/BEACH PO group: Paul Lethaby Research Associate UH/PO Christin Shacat Research Associate UH/PO Jefrey Snyder Marine Technician UH/PO Sarah Yasui Volunteer UH/PO Nicolas Petrochilos Volunteer UH/PO John Fitzpatrick Volunteer UH/PO Others: Janice Jones Technician UCSB Sara Yeo Technician UH/HIMB Gordon Walker Teacher UH/CMORE Doug Weidman Teacher UH/CMORE Dan Hendricks Teacher UH/CMORE Kate Achilles CMORE Educator UH/CMORE Kuhio Vellalos Marine Technician OTG Elly Speicher Marine Technician OTG

39 3. GENERAL SUMMARY All of the operations during the cruise were conducted as planned and only minor delays were experienced. One 500 m weight cast was performed with a 1,300 lb. weight and one 1000-m CTD cast was conducted at Station Kahe (1). Two near-bottom deep casts, thirteen 1000-m CTD casts, one 350-m CTD cast, and one 200-m CTD cast were conducted at Station ALOHA (2). One one hour 200 m yo-yo cast was conducted near the WHOTS mooring (Station 52). The array of floating sediment traps, the gas array, and the primary production array were deployed and recovered without any major incidents. All of the arrays drifted to the NNE of ALOHA. Primary Production Array 2.7nm; N W. Gas Array 4nm; N W Sediment Trap Array 12nm; N W Six net tows were completed, three were conducted at night, and three during the day. The AC9/FRRf was deployed around noon three times, and once at night. The PRR was deployed three times around noon. The ADCP, thermosalinograph, pco2 system, and the two anemometers ran without interruption throughout the cruise. Winds ranged from the SE at 10knots to the W at 15knots to NNW at 5knots during the course of the cruise with swells between 4-12ft. 4. R/V KILO MOANA, OFFICERS AND CREW, TECHNICAL SUPPORT The R/V Kilo Moana continues to maintain excellent ship support for our work. The Captain and crew were most helpful and accommodating throughout the cruise. They were very flexible in receiving changes to our operational schedule. Throughout our cruise, the entire crew showed enthusiasm, concern, and dedication to our scientific mission. Technical support during this cruise was excellent. OTG personnel were available at any time to assist in our work and helped keep operations running smoothly.

40 5. DAILY REPORT OF ACTIVITIES (HST) February 21, 2008 Loading Day Heavy equipment, the blue storage van and all hand carried gear was loaded during this day. CTD wire was reterminated. February 22, 2008 Departed Snug harbor at 0900hrs Science and Safety meeting at 0945hrs Fire and boat drill at 1030hrs, all science personnel attended. After, all of the new personnel attended a meeting about the proper way to deploy life rafts and how to put on the survival suits. Arrived station Kahe at 1130hrs, conducted a weight cast at 1140hrs, PRR at 1220hrs, a 1400-m CTD cast at 1300hrs and Go-Flow cast at 1430hrs. Deployed magnetometer at 1440hrs Underway to station ALOHA at 1450hrs Recovered magnetometer at 2230hrs Arrived station ALOHA at 2245hrs The sediment trap array was deployed at 2327hrs. The array was deployed at the location of N, W. February 23, 2008 One 200-m CTD cast was conducted at 0030hrs. This was followed by a 1000-m CTD cast at 0210hrs to collect water for the primary productivity experiment. The primary production array was deployed at 0430hrs at the location of N, W At 0550hrs a near-bottom PO/CTD cast was conducted. A net tow was conducted by Blake Watkins at 1015hrs. The 36hr burst period started at 1115hrs with a 1000-m CTD cast. The second CTD cast of the period started at 1400hrs. The ISUS was installed in the rosette and connected before the first CTD cast of the 36hr period. A second net tow was conducted at 1300hrs by Blake Watkins. The primary production array was recovered at 1915hrs on Feb. 23 rd. The array drifted about 2.7 nautical miles N from the center of station ALOHA at the location of N W. As part of the 36hr period, three more 1000-m CTD casts were conducted. Another net tow was completed by Blake Watkins at 2205hrs Weather conditions observed at 1500; winds from the SE at 10 knots, seas 5-8ft, cloud cover around 2/8, mixed layer depth ~125m.

41 February 24, 2008 The gas array was deployed at 0435hrs at the location of N W One PRR cast was conducted at 1228hrs today. One AC-9/FRRf cast was conducted after the PRR cast at 1250hrs. Three net tows were completed by Blake Watkins at 0100hrs, 1015hrs and 2245hrs. Seven more 1000-m CTD casts were conducted as part of the 36hr burst period before ending the burst period with a second deep cast at station ALOHA at 2345hrs. After cast 15 the ship transited to pump tanks but on the way back in the circle a longline fishing vessel made the KM take a long route back in the circle. Because of this the net tow and second deep cast were late. Weather conditions observed at 1430hrs; winds from the W at 15 knots, seas 4-6ft and cloud cover 8/8. February 25, 2008 The sediment trap array was recovered at 0635hrs after drifting 12nm to the NE from the center of ALOHA. The array was recovered at N W. The gas array was recovered at 0730hrs after drifting 4nm to the NE from the center of ALOHA. The array was recovered at N W. One final 200m CTD cast was added at station ALOHA because the WHOTS mooring was tending out of the circle. One PRR cast was conducted at station ALOHA at 1100hrs. One AC-9/FRRf cast was conducted at 0345hrs. Two AC-9/FRRf casts were conducted in the afternoon at 1130hrs and 1230hrs. One 200-m yo-yo CTD cast was conducted near the WHOTS mooring at 1400hrs. Upon recovery, the CTD wire came in contact with the crane cab s ladder which compromised the wire s integrity. The ladder was not properly stowed for CTD recovery operations. Roughly 50m of wire was removed and the wire was reterminated. A near-bottom CTD cast was conducted at station Kaena at Weather conditions at 1600; winds from the NNW at 5 knots, seas 10-12ft and cloud cover 2/8. February 26, Arrived snug Tied up after removing the OTG van, complete offload.

42 HOT program sub-components: Investigator: Project/Institution: Dave Karl Core Biogeochemistry/UH Roger Lukas Hydrography/UH Bob Bidigare HPLC pigments/uh Mike Landry Zooplankton dynamics/uh Mark Abbott/Ricardo Letelier Optical measurements/osu Ancillary programs: Investigator: Project/Institution: Charles Keeling CO2 dynamics and intercalibration/sio Paul Quay DI13C and O isotopes/uw Penny Chisholm Prochlorococcus population dynamics/mit Zehr/Church/Montoya Diversity and activities of nitrogen-fixing microorganisms/uh CMORE PI s Microbial RNA/DNA collection/cmore Mark Brzezinski Silica production and dissolution rate measurements/ucsb Additional programs Investigator: Project/Institution: Mike Rappe Marine bacterioplankton community structure/uh Kate Achilles CMORE Education