Quarterly Report. 4 December February 2014

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Quarterly Report Faculty of FORESTY 4 December 2013 28 February 2014 School of AGRICULTURAL, FOREST, AND ENVIRONMENTAL SCIENCES College of AGRICULTURE, FORESTRY & LIFE SCIENCES Baruch Institute of Coastal Ecology and Forest Sciences Hwy. 17 N. 177 Hobcaw Rd. Georgetown, SC 29440 (843) 546-1013 FAX (843) 546-6296 To: William Bailey and Margarett G. McIntosh Planning Division Savannah District US Army Corps of Engineers 100 W. Oglethorpe Ave. Savannah, GA 31401 Office of Naval Research 100 Alabama Street, SW Suite 4R15 Atlanta, GA 30303-3104 Sherry Whitaker U.S. Army Engineer Research and Development Center 3909 Halls Ferry Road Vicksburg, MS 39180 11 March 2014 By: Jamie Duberstein Baruch Institute of Coastal Ecology and Forest Science Clemson University PO Box 596 Georgetown, SC 29442 Cell: 706-410-6722 Quarterly Report, Spring 2014, p.1

US Army Corp. of Engineers: Please see below for an executive summary, then detail, of major accomplishments, actions, and progress associated with the vegetation and salinity monitoring within the Savannah National Wildlife Refuge. This work is done under Cooperative Agreement Number W912HZ-14-2-0002 under the terms of the Piedmont South Atlantic Coast (PSAC) Cooperative Ecosystems Studies Unit (CESU). The Cooperative Agreement Title is Identifying and Evaluating Impacts to Wetlands from the Savannah River Estuary. Respectfully, Jamie Duberstein Clemson University Executive Summary: A major site reconnaissance was conducted in January, and the 12 marsh monitoring sites were chosen. Seven of these areas were monitored previously by Dr. Kitchens at the Florida Coop Unit. The five new marsh sites were agreed mutually agreed upon by Duberstein and Bailey, under the agreement that three forested areas also be monitored, locations to be determined in the summer, with annual tree/shrub vegetation surveys. The water sensor vendor was quite amenable financially, allowing us the ability to monitor all 15 sites for aboveground and belowground salinity, and water level. Enough water sensors are in-hand for 11 of the 15 areas, with the remaining expected soon. Wells for the sensors are under construction. Sensors will be deployed immediately before, or during, the April marsh vegetation sampling. The processes for purchasing a truck and boat are underway, and we will soon hire our temporary technician. A map of the 12 marsh areas is provided as Figure 1 in the addendum (reconnaissance report). Specific sample locations are provided in Table 1 at the end of this report. Quarterly Report, Spring 2014, p.2

December: Official start date is 04 December 2013, according to the US Army Corp of Engineers, Savannah District (USACE). Funds were received in December, and an account had been created at Clemson University by 18 December 2013. Little was able to be done between the 19 December and the new year. January: Obtained quotes for a boat/motor/trailer package from vendors in Georgia and South Carolina. Began processing of the paperwork and ordering of water sensors. Got a position approved with Clemson Human Resources, and advertised for the temporary technician to be hired under this grant. Field reconnaissance was done in January, and the field reconnaissance report is included as an addendum after the February summary. February: Finalized the order for the water level sensors, and received enough sensors to fully outfit 11 of the 15 monitoring areas. The remaining sensors are expected late March. Purchased flat PVC that will be used as the base for mounting wells for underground and aboveground water sampling. Construction of the wells is currently underway. Obtained 3 bids for a boat/motor/trailer, all with the same specifications, and submitted them to Clemson Purchasing. A sealed bid process will be used to determine the specific vendor, at which point the equipment can be ordered. I anticipate that I will have to rent a boat for the water sensor deployment and April vegetation sampling; this is readily available. Quarterly Report, Spring 2014, p.3

Submitted a purchase request to the state contract vendor for a Chevrolet 1500 4x4 truck able to utilize the launching facilities at the refuge, which are often muddy due to tidal action and relatively little use. Vinyl seats were requested, in lieu of cloth. Delivery is expected May or April 2014. Received 29 applicants for the Research Technician (term) position that is associated with this project. A short list of 4 applicants was selected for interview in March. Interviews are currently underway. Coordinated with Tommy Jordan the site locations and my anticipated data format and storage needs. Set a date for the April sample (3 rd 12 th ), the 1 st of the 4 vegetation samples. Two Independent Contractors, outlined in the proposal, are assisting. I attended a Motorboat Operator Certification Course and obtained my MOCC certification. I can now be authorized to operate DOI boats, including those of the Savannah Refuge. Quarterly Report, Spring 2014, p.4

Addendum: Reconnaissance Report Summary: Twelve tidal marsh and three tidal forest monitoring sites were chosen, all located within the Savannah National Wildlife Refuge. Seven marsh sites are identical to those historically monitored by Dr. Wiley Kitchens (US Geological Survey Florida Coop Unit). Five marsh sites are new, and locations of monitoring sites have been agreed upon by both the Investigators and the US Army Corp of Engineers. Files associated with this report are provided to the USACE Savannah District via the Google Drive link that was provided. These files include: this report, which includes a map of site locations and a table with site coordinates, a Google Earth *.KMZ file with marsh sites, an Excel spreadsheet containing the coordinates for all marsh sample sites, and four pictures from each marsh sample site. Pictures of marsh sample sites were taken during the January 2014 reconnaissance trip. Locations of the three forest monitoring stations will be determined following leaf-out (i.e., when leaves are on the trees). Quarterly Report, Spring 2014, p.5

Figure 1: Locations of the twelve marsh monitoring areas. Icons: H depicts historic sites, N depicts new sites, I marks the ecotone between tidal marsh and tidal forest (a mix of both). Quarterly Report, Spring 2014, p.6

Sample site locations Nomenclature: Marsh sample site names are a combination of river branch, transect, and plot. For example: Back 1 2A Back = Back River (versus Middle or Front) 1 = area number 1 of the Back River areas; numbering is intraspecific to river branches and begins with 1 as the most upstream area 2 = transect number; there are three transects per area, but note that some historic study areas have transects #ed 4, 5, and 6 A = plot along the transect. There are 3 plots located 20, 70, and 120 meters from the bank of the nearby tidal creek Historic marsh sample sites: Dr. Wiley Kitchens (USGS Florida Coop Unit) monitored seven marsh areas during the years 1985-2006. We will continue to monitor all seven historically monitored areas (Figure 1). However, while Kitchens maintained six transects per area (there were/are seven areas), our study design calls for three transects per area, with the establishment of five new study areas. The choice of which transects to retain for this new monitoring effort was made based on how well transects represent the predominant vegetation of the area, making sure they will all be experiencing the same (or as similar as reasonably possible within field sites) hydrology as the onsite water level (and salinity) recorder. Historic sample sites were reestablished at the same location, made easier by the already exiting site markers (i.e., pvc poles); locations were also confirmed by finding the historic salinity well at each marker, and by comparing coordinates (loaded into a GPS) provided in Tidal Wetland Resource Utilization Studies (Kitchens et al. 2003). Some sample site coordinates were unacceptably different than the coordinates we read from our instrumentation during our January 2014 field reconnaissance trip. This was limited to three sample sites, and differences were on the order of 15-20 ft. Specific sites were Back 1 5B; Back 3 3C; and Back 3 4C. Because of the increased accuracy afforded by more advanced GPS technology, coordinates given in Table 1 reflect our adjustments to these three sample sites. Although historic transects were chosen (from the 6 available) in a manner to best represent the hydrology of the river section it is named after (i.e., Back, Middle, Front), some sample sites are Quarterly Report, Spring 2014, p.7

currently occupied by vegetation communities that are outside of the target assemblages. Back 2 transects 2 and 3 are up on the berm (a microtopographical high area typically associated with creek edges) and are dominated by berm species such as cutgrass and wild rice. Back 1 transect 4 pole C is overtaken by a shrubby hazel alder (alnus serrulata) tree, but there are nearby patches of marsh. A large portion of this Back 1 site abuts the ecotone between open marsh and dense shrubs, which later transitions to freshwater tidal forest. New marsh sample sites: Five new marsh study areas were identified during the reconnaissance trip. After doing an on-site visit to the Savannah National Wildlife Refuge, we met with Mackie McIntosh and Bill Bailey (USACE Savannah District) and adjusted our efforts according to locations mutually agreed upon at this 20 January 2014 meeting. My field crew and I then established the five new study areas by putting out site markers (A, B, and C samples sites) along each of the three transects. Pictures of the vegetation were also taken at each sample site. One new Back River area was established between the historic Back 3 and Back 4; it is called Back 3.5. One new Front River area was established downstream of Front 1; it is called Front 2 (Figure 1). Three Middle River areas were established, all downstream of the historic Middle 2; they are named Middle 3, Middle 4, and Middle 5. Forested sites (all new): Three forested areas will be monitored, and each area will have two (20 x 25 m) sample sites. Vegetative surveys will be conducted yearly, and include identification of all tree species, and a measurement of their diameter at breast height (DBH). All forest areas will be located nearest the Front River (versus Middle or Back), with the site farthest upstream (presumed to be lowest in salinity, if a gradient is identified) already established (Fig. 1), and currently being monitored as part of a separate 10+ year study. Two additional forest areas will be identified after leaf-out (i.e., when leaves are present on the trees), with placement roughly between the established upstream area and the marsh/forest ecotone (Fig. 1). Quarterly Report, Spring 2014, p.8

Pictures: Four pictures were taken at each of the marsh sample sites during the reconnaissance trip. These are helpful for visualizing not only the predominant vegetation during this reconnaissance trip, but also for identifying those sample sites that are directly on an ecotone between two communities. It is likely that vegetative sampling will capture both communities at the ecotone, so we will need to be cognizant of this information during our interpretation of analysis results. Site pictures have been uploaded in *.jpg format to the folder Jamie Marsh Monitoring, available via the Google Drive link that was provided by Ms. McIntosh on 24 January 2014. Sites are identifiable by their file names, following the nomenclature outlined above. Water Sensor Locations: Water level and salinity sensors will be deployed to the same sample sites as the YSI sondes were during previous marsh monitoring (i.e., Dr. Kitchens). Locations of the monitoring wells for marsh sites (new and historic) are indicated in the Recorders column of Table 1, and are displayed as unique icons in the Google Earth *.kmz file. Monitoring wells in the forested areas will be placed between the two (20 x 25 m) sites. ` Quarterly Report, Spring 2014, p.9

Table 1. Locations of marsh monitoring sample sites along the Savannah River. Coordinates are in the UTM system with datum WGS84. Water Level Site Zone Easting Northing Status Recorder? BACK 1 2A 17S 488298 3561718 Historic BACK 1 2B 17S 488296 3561760 Historic BACK 1 2C 17S 488289 3561806 Historic BACK 1 4A 17S 488134 3561729 Historic BACK 1 4B 17S 488132 3561772 Historic Yes BACK 1 4C 17S 488122 3561813 Historic BACK 1 5A 17S 487982 3561717 Historic BACK 1 5B 17S 487981 3561760 Historic BACK 1 5C 17S 487977 3561811 Historic BACK 2 2A 17S 488024 3559680 Historic BACK 2 2B 17S 488020 3559641 Historic BACK 2 2C 17S 488020 3559610 Historic BACK 2 3A 17S 487893 3559684 Historic BACK 2 3B 17S 487892 3559650 Historic Yes BACK 2 3C 17S 487892 3559602 Historic BACK 2 4A 17S 487766 3559697 Historic BACK 2 4B 17S 487771 3559645 Historic BACK 2 4C 17S 487771 3559606 Historic BACK 3 2A 17S 488326 3557543 Historic BACK 3 2B 17S 488348 3557498 Historic BACK 3 2C 17S 488366 3557452 Historic BACK 3 3A 17S 488130 3557560 Historic BACK 3 3B 17S 488123 3557522 Historic Yes BACK 3 3C 17S 488122 3557477 Historic BACK 3 4A 17S 488063 3557575 Historic BACK 3 4B 17S 488062 3557536 Historic BACK 3 4C 17S 488057 3557488 Historic BACK 3.5 1A 17S 488523 3556592 New BACK 3.5 1B 17S 488502 3556547 New Yes BACK 3.5 1C 17S 488480 3556501 New BACK 3.5 2A 17S 488365 3556684 New BACK 3.5 2B 17S 488336 3556642 New BACK 3.5 2C 17S 488307 3556600 New BACK 3.5 3A 17S 488537 3556647 New BACK 3.5 3B 17S 488560 3556691 New BACK 3.5 3C 17S 488584 3556736 New BACK 4 2A 17S 489479 3554694 Historic BACK 4 2B 17S 489496 3554657 Historic BACK 4 2C 17S 489510 3554618 Historic

Site Zone Easting Northing Status Recorder? BACK 4 5A 17S 489561 3554771 Historic BACK 4 5B 17S 489542 3554819 Historic BACK 4 5C 17S 489529 3554860 Historic BACK 4 6A 17S 489722 3554881 Historic BACK 4 6B 17S 489731 3554924 Historic Yes BACK 4 6C 17S 489739 3554969 Historic FRONT 1 1A 17S 486093 3561232 Historic FRONT 1 1B 17S 486084 3561197 Historic Yes FRONT 1 1C 17S 486068 3561156 Historic FRONT 1 2A 17S 486049 3561239 Historic FRONT 1 2B 17S 486033 3561210 Historic FRONT 1 2C 17S 486019 3561169 Historic FRONT 1 3A 17S 485992 3561241 Historic FRONT 1 3B 17S 485979 3561217 Historic FRONT 1 3C 17S 485959 3561175 Historic FRONT 2 1A 17S 485576 3559120 New FRONT 2 1B 17S 485576 3559171 New FRONT 2 1C 17S 485576 3559221 New FRONT 2 2A 17S 485640 3559116 New FRONT 2 2B 17S 485643 3559166 New Yes FRONT 2 2C 17S 485646 3559216 New FRONT 2 3A 17S 485705 3559111 New FRONT 2 3B 17S 485709 3559161 New FRONT 2 3C 17S 485713 3559210 New MIDDLE 1 2A 17S 487286 3561722 Historic MIDDLE 1 2B 17S 487282 3561761 Historic Yes MIDDLE 1 2C 17S 487276 3561801 Historic MIDDLE 1 5A 17S 487425 3561715 Historic MIDDLE 1 5B 17S 487437 3561746 Historic MIDDLE 1 5C 17S 487445 3561794 Historic MIDDLE 1 6A 17S 487503 3561705 Historic MIDDLE 1 6B 17S 487501 3561742 Historic MIDDLE 1 6C 17S 487501 3561774 Historic MIDDLE 2 3A 17S 487395 3560878 Historic MIDDLE 2 3B 17S 487393 3560922 Historic MIDDLE 2 3C 17S 487388 3560969 Historic MIDDLE 2 4A 17S 487467 3560883 Historic MIDDLE 2 4B 17S 487468 3560926 Historic Yes MIDDLE 2 4C 17S 487467 3560971 Historic MIDDLE 2 5A 17S 487546 3560883 Historic MIDDLE 2 5B 17S 487536 3560929 Historic MIDDLE 2 5C 17S 487525 3560972 Historic

Site Zone Easting Northing Status Recorder? MIDDLE 3 1A 17S 487418 3560198 New MIDDLE 3 1B 17S 487418 3560249 New MIDDLE 3 1C 17S 487419 3560299 New MIDDLE 3 2A 17S 487581 3560192 New MIDDLE 3 2B 17S 487585 3560242 New Yes MIDDLE 3 2C 17S 487590 3560291 New MIDDLE 3 3A 17S 487741 3560186 New MIDDLE 3 3B 17S 487744 3560236 New MIDDLE 3 3C 17S 487747 3560287 New MIDDLE 4 1A 17S 487312 3558402 New MIDDLE 4 1B 17S 487306 3558352 New Yes MIDDLE 4 1C 17S 487299 3558303 New MIDDLE 4 2A 17S 487522 3558367 New MIDDLE 4 2B 17S 487515 3558317 New MIDDLE 4 2C 17S 487508 3558267 New MIDDLE 4 3A 17S 487326 3558468 New MIDDLE 4 3B 17S 487331 3558507 New MIDDLE 4 3C 17S 487338 3558556 New MIDDLE 5 1A 17S 486833 3558093 New MIDDLE 5 1B 17S 486800 3558056 New MIDDLE 5 1C 17S 486768 3558017 New MIDDLE 5 2A 17S 486776 3558131 New MIDDLE 5 2B 17S 486743 3558093 New Yes MIDDLE 5 2C 17S 486711 3558054 New MIDDLE 5 3A 17S 486708 3558180 New MIDDLE 5 3B 17S 486677 3558141 New MIDDLE 5 3C 17S 486647 3558102 New