AUTOMATED MEDIA PREPARATION

AUTOMATED MEDIA PREPARATION

AUTOMATED MEDIA PREPARATOR for all types of agars, broths, diluents - 2.5 to 30 Liters - Fast heating up to 121 C - High T accuracy: Temperature mapping from 1 to 3 points deep inside the media - Almost no dead volume: 130ml - New Homogenizing technology Wide viscosity range High security: - Safety cover locked above 80 C - Audible & visible alarms - Anti-shock (ABS PC plastics) body Former technology - High prevention of contamination: Uphill Prefiltration (carded cotton) Downhill filtration (disk decontamination filter). *Money saving on filters: Prefilter prevents from early clogged filter (about 3 times less changes). Traceability - Export all the data in.csv file (EXCEL TM ) through USB B C C D - Easy cleaning of the bowl Bottom opening with sufficient space to place a recipient and rinse from above) A D A E Cut views A. Bowl B. Priming tube C. Magnetic stirrer D. Draining plug / Axe basis / Dispensing plug No valve = no bacteria hideout E. Evacuation port (smooth surface) A+E = One piece part, preventing contamination risks. Easy to access and clean. All these mentioned items are manufactured in 316L stainless steel Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 2

Specifications SPECIFICATIONS Media preparation capacity From 2.5L to 30L Sterilization temperature 95 C to 125 C Sterilization time 1 to 99min Distribution temperature 25 C to 80 C Temperature precision ± 0.5 C Dead volume < 130ml Blood agar preparation Yes Country of origin 100% France Stored programs 50 Control panel LCD Touch screen Traceability CSV file through USB port High mobility / stability 4 large lockable wheels DIMENSIONS W x H x D 585 x 1053 x 735 mm Electrical supply 400V - 50-60Hz -16A or 230V 50-60Hz 22A (by phase) Made in France Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 3

SOLID MEDIA LIQUID MEDIA DILUENT A large selection of high quality dehydrated media. Made in France REF. PRODUCT DESCRIPTION DSHB3004 Buffered Peptone Water ph = 7 (500 g) DSHB3005 Pharmacopoeia Diluent (500 g) DSHB3024 LT100 Broth (500 g) DSHB3041 Peptone Salt Broth (500 g) DSHB3048 Buffered Peptone Water ph7.2 (500 g) DSHB3002 Tryptic Soy Broth (TSB) (500 g) DSHB3008 Mac Conkey Broth (500 g) DSHB3014 Rappaport-Vassiliadis Broth (RVS) (5 KG) DSHB3015 Brilliant Green Bile Broth (BGBLB) (500 g) DSHB3018 Fraser Broth (500 g) (to be ordered with DSHB3049 or DSHB3050) DSHB3025 Müller-Kauffmann Tétrathionate Novobiocine Broth (MKTTn) (500 g) DSHB3026 Mossel Broth (500 g) DSHB3033 Bolton Broth Campylobacter (to be ordered with DSHB3053) (500 g) DSHB3036 Sabouraud Broth (500 g) DSHB3040 Thioglycolate Broth (500 g) DSHB3055 Rappaport Vassiliadis Soy Broth (RVS) (500 g) DSHB3001 Tryptic Soy Agar (TSA) (500 gr) DSHB3003 Sabouraud Dextrose Agar (SDA) (500 g) DSHB3007 Mac Conkey Agar (500 g) DSHB3009 R2A Agar (500 g) DSHB3010 Cetrimide Agar (Pseudomonas Selective Agar) (500 g) DSHB3011 Xylose Lysine Desoxycholate Agar (XLD) (500 g) DSHB3012 Mannitol Salt Agar (500 g) DSHB3013 Nutrient Agar (500 g) DSHB3016 Brain Heart Infusion Agar (500 g) DSHB3017 Columbia Agar (500 g) DSHB3022 Desoxycholate Agar (500 g) DSHB3023 Kligler Iron Agar (500 g) DSHB3027 Man-Rogosa-Sharpe Agar (MRS) (500 g) DSHB3028 MYP Bacillus cereus Agar (500 g) DSHB3029 Bromocresol Purpule Glucose Agar (500 g) DSHB3030 OGYE Agar (500 g) DSHB3031 Listeria Oxford Agar (to be ordered with DSHB3051) (500 g) DSHB3032 Listeria Palcam Agar (to be ordered with DSHB3052) (500 g) DSHB3034 Tryptic Glucose Yeast Agar (PCA) (500 g) DSHB3035 Potato Dextrose Agar (PDA) (500 g) DSHB3037 Sabouraud Dextrose Agar (SDA) + chloramphenicol (500 g) DSHB3038 Tryptone Bile X-Glucuronide Agar (TBX) (500 g) DSHB3039 TCBS Kobayashi Agar (500 g) DSHB3042 Tryptone-Sulfite Cyclosérine Agar (TSC) (to be ordered with DSHB3021) (500 g) DSHB3043 Violet Red Bile Glucose Agar (VRBG) (500 g) DSHB3044 Violet Red Bile Lactose Agar (VRBL) (500 g) DSHB3045 Chloramphenicol Glucose Yeast Extract Agar (YGC) (500 g) DSHB3046 Bile Esculine Azide Agar (500 g) DSHB3047 Endo Agar (500 g) DSHB3054 Mueller Hinton (500 g) DSHB3056 Columbia Agar Base (500 g) DSHB3019 Lyophilised Rabbit Plasma Fibrinogen (RPF Supplement) (4 x 100 ml) DSHB3020 Pseudomonas CFC Supplement (10 x 500 ml) Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 4

AUTOMATED MEDIA Plate pourer stacker Load up to 440 plates & start the automated dispensing 440 ready to use plates in 30 mins (15ml) DISTRIWEL is a real WALK AWAY device. - Your culture media is accurately poured by a Masterflex TM peristaltic pump - Ensures a flat poured media (bubble level integrated) - The Built-in Peltier cooling effect reduces condensation and solidification time Traceability - Print up to 25 characters (2 lines) on the Petri dish (optional laser printer) Contamination prevention (UV lamp) while pouring Plexiglas protection cover Quick removable carrousel & transfer disk for total cleaning Small footprint Easy loading system (easy access to the 11 columns of 40 plates) Front peristaltic pump Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 5

Specifications DISTRIWEL SPECIFICATIONS Dishes/hour for Ø 90mm Up to 750 Capacity of the carrousel 440 dishes Fast plate cooling Built-in Peltier effect Contamination prevention Built-in UV lamp Pour plates function Pouring accuracy ±1% Pouring range 1 99 ml Stack plates function Peristaltic pump Integrated Masterflex pump Stored programs 40 Control panel LCD Touch screen Country of origin 100% France Traceability Laser printer (LINX CJ400) Printed Petri dishes Footswitch (for manual dispensing) (optional) DIMENSIONS W x H x D 808 x 940 x 689 mm Electrical supply 100-250v 50-60 Hz 10 A Power supply 150 W Made in France Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 6

WRAPPING MACHINE The Minima wrapping machine wraps your Petri dishes in packs of 10 (1 column of 10 or 2 columns of 5). A.B.E has carefully selected the adequate wrapping film, to avoid any condensation. Freshly poured with your DISTRIWEL, dishes are packed, ready to be transported. Specifications PERFORMANCES Polyolefin rolls Delivered in 6 rolls of 1250 m - 15µm - 300 mm Wrapping film Electrical power supply Power Maximum product size Dimensions with the lid opened Dimensions with the lid closed Weigh (Net/Gross) Programs 200/230V 1 phase 1.2 kw 350 x 240 x 140 mm 930 x 550 x 580 mm 930 x 550 x 370 mm 36/48 kg 10 preset Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 7

MULTIFUNCTIONAL COMPACT Peristaltic pump - Fast: 9 ml 1 second 18 ml 3 seconds 225 ml 10 seconds - Accurate: ± 0.6 ml (w/ tubing Ø 6.4 mm) ± 0.4 ml (w/ tubing Ø 4.8 mm) ± 0.2 ml (w/ tubing Ø 3.2 mm) - Compact & light: 3.8kg Easy transportable - Few steps and the calibration is done. - Calibration reminder: The DOSYWEL can also remind you every x sample, to perform a calibration - Up to 20 programs - Editable: o Volume (1 to 9999ml) o Number of doses (1 to 999) o Time for interval (0.1 to 60.0 seconds) o Pump speed (1 to 130% = 10 to 550 rpm) o Diluent (batch, name, volume) o Calibration mode (calibration starting the program, reminder each x sample). - From MANUAL TO AUTOMATED: The DOSYWEL can be plugged to the automated XYZ system, POLYWEL 3D. It drives the robot to dispense until 1000 tubes (9ml) per hour. - ADDITIONAL PUMPS: Add and drive up to 3 additional pumps (Turbo to dispense faster) or (to dispense different media) - Data export to USB (CSV file) - Linkable to ticket printer, flash code & bar code reader. - Prepared to LIMS bidirectional communication Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 8

Specifications PERFORMANCES Distribution volume 0.1 to 9999.9 ml Pause time 1 99 seconds Adjustable speed 1 130% 10 550 rpm Connectivity POLYWEL 3D (automated distribution) Auto calibration scale Communication USB port. Ethernet Internal tube dimensions 0.8 / 1.6 / 3.2 / 4.8 / 6.4 / 8.0 mm External tube dimensions 1.6 mm Country of origin 100% France Programs 20 Rotation way Left or right Foot switch YES (sold separately) DIMENSIONS W x H x D 210 x 200 x 240 mm Electrical supply 100-240 V 50-60 Hz Power supply 90 W TRACEABILITY Ticket printer (optional) USB export (optional) TUBE DIAM. ACCURACY AT 100% SPEED REPEATABILITY Tube Ø 1.6mm 9ml : ± 0.037 ml / 18 ml : ± 0.037 ml / 225ml : ± 0.037 ml ± 0.007 ml Tube Ø 3.2mm 9ml : ± 0.159 ml / 18 ml : ± 0.159 ml / 225ml : ± 0.159 ml ± 0.055 ml Tube Ø 4.8mm 9ml : ± 0.365 ml / 18 ml : ± 0.365 ml / 225ml : ± 0.365 ml ± 0.060 ml Tube Ø 6.4mm 9ml : ± 0.530 ml / 18 ml : ± 0.530 ml / 225ml : ± 0.530 ml ± 0.070 ml Made in France Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 9

AUTOMATED XYZ filling system (to be completed with DOSYWEL) - Completely driven by the DOSYWEL peristaltic pump - Preset and editable configuration - Visual alarms (end of cycle, status, alerts. - Removable platform cover - Stainless steel tray - Autoclavable racks - Wide effective dispensing area: 50 x 85 cm (XY) = surface for 1000 tubes (9-12ml / Ø18mm) - Available dispensing height: 0 25 cm (Z) Adjustable the height to the sample and avoid any risk of splashing. - Dispense up to 1900 tubes / hour (9ml tubes) - Small racks (up to 8 = 960 tubes) OR Large racks (up to 4 = 1008 tubes) - Adaptable to all kind of containers (tubes and flasks). - Data export to USB (CSV file) - Linkable to ticket printer, flash code & bar code reader. - Prepared to LIMS bidirectional communication Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 10

Specifications PERFORMANCES Arm movement X, Y and Z (mechanical) Maximum height dispensing 0 250 mm Max. capacity of tubes on the tray 1008 tubes (9ml) Internal tube dimensions 0.8 / 1.6 / 3.2 / 4.8 / 6.4 / 8.0 mm External tube dimensions 1.6 mm Country of origin 100% France Programs 20 Removable platform cover Autoclavable racks DIMENSIONS Dimensions H x W x L 610 x 610 x 1130 mm Useful dimensions (dispensing area) H x W x L 250 x 510 x 960 mm Power supply 230V +/- 10% - 50-60 Hz TRACEABILITY Ticket printer (optional) Flash & bar code reader (optional) Data export to USB (optional) Made in France Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 11

Contact us: Tel: +33(0)2 40 51 79 53 Email: contact@abioexpertise.com Empowered from our people skills and experiences, we, ALLIANCE BIO EXPERTISE, together with our customers and partners decided to manufacture Microbiology automated equipment around essential values: Customer Focus, Service, Loyalty, Innovations Ask your local partner for a personal demonstration! Alliance Bio Expertise ZA de Courbouton - Le Tremplin 35480 GUIPRY-MESSAC France 12