HARLECO Dyes & Stains Microscopy products to support your success

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HARLECO Dyes & Stains Microscopy products to support your success The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. A

For over 90 years, HARLECO brand dyes and stains have offered our customers outstanding contrast, color, and clarity in their sample specimens. HARLECO stain solutions offer not only the classical formulations, but also many new and improved modifications developed from years of experience. We use Certified Biological Stains and cgmp manufacturing for all HARLECO products to provide you with the highest quality stain solutions. In addition, we offer a MIDAS III Automated Stainer and a complete line of products for sample preparation. B

Hematology Reagents to identify diseases of the blood and blood forming organs The HARLECO product line offers ready to use staining solutions for blood and bone marrow samples that yield intense and reproducible results. Reproducibility can be enhanced by the use of phosphate buffer solution, which stabilizes diluted staining solutions. We offer buffers with different ph levels, so that you may obtain optimal staining results. For higher slide staining throughput or for preparing urgent lab samples, Hemacolor, the fast staining kit, can give very clean results in less than one minute. Product Size Catalog No. HARLECO Giemsa Stain Solution, Azure B 500 ml CA15204-142 HARLECO Giemsa Stain Solution, Modified Azure Blend HARLECO Giemsa Stain Solution, Original Azure Blend CA15204-146 CA15204-144 Wright staining-blood smear Giemsa Stain Solution 500 ml CAR03055-74 CAR03055-76 CAR03055-82 HARLECO Jenner Stain Solution CA34172-038 HARLECO May-Grunwald Solution HARLECO Wright Stain Solution HARLECO Wright Giemsa Solution, APHA HARLECO Wright Giemsa Solution, Fucillo 10 L 10 L 500 ml CA15204-200 CA15204-292 CA15204-228 CA15204-230 CA15204-224 CA15204-232 CA15204-226 CA34172-051 CA15204-158 CA15204-159 HARLECO Wright Giemsa Stain Pack CA34172-110 HARLECO Wright Stain Pack CA34172-112 Leishman Stain Solution CAR03087-76 HARLECO Hemacolor Stain Set 1 PAC CA15204-176 HARLECO Hemacolor Solution I CA15204-170 HARLECO Hemacolor Solution II CA15204-172 HARLECO Hemacolor Solution III CA15204-174 HARLECO Buffer, Phosphate ph 6.4 HARLECO Buffer, Phosphate ph 6.8 HARLECO Buffer, Phosphate ph 7.0 CA34171-012 CA34171-000 CA34171-002 CA11214-838 CA1219-75 CA1219-85 1

Cytology Reagents for brilliant and highly reproducible sensitive results To stain gynecological and clinical specimens, use standard Papanicolaou sample staining with hematoxylin Gill s solution. In addition, you can rely on our high quality alcohol and the xylene substitute, Neo-Clear, to further improve your results. Product Size Catalog No. HARLECO Hematoxylin, Gill I CA15204-178 HARLECO Hematoxylin, Gill II CA15204-180 HARLECO Hematoxylin, Gill III CA15204-182 Papanicolaou stain gynecological sample HARLECO Hematoxylin, Harris Alum, Hg Free HARLECO EA Solution, Gill (with Eosin and Light Green) 500 ml CA15204-150 CA15204-152 CA15204-184 HARLECO Orange G Solution, Gill CA15204-186 HARLECO Papanicolaou, OG-6 HARLECO Papanicolaou, EA-65 HARLECO Papanicolaou, EA-36 HARLECO Papanicolaou, EA-50 10 L 10 L CA15204-208 CA-15204-206L CA15204-276 CA15204-210 CA34172-058 CA15204-270 CA15204-212 10143-158 CA15204-216 HARLECO Blueing Reagent CA34172-018 HARLECO Acid Alcohol, 70% (3% HCl) CA15204-266 HARLECO Alcohol, 70% 65350-85 Neo-Clear Xylene Substitute 65351-85 2

Histology Reagents for brilliant and stable results from the first to the last step in the process Precise specimen preparation is a necessity to get good slides for diagnostic work. We offer all of the products needed for preparing histology samples, including top quality fixing media and high purity solvents. The HARLECO stain results are color stable, reproducible and offer sufficient sensitivity to reveal all structural details. Product Size Catalog No. HARLECO Eosin Y Solution, Alcoholic Saturated CA15204-130 HARLECO Eosin Y Solution, Aqueous, 1% PDC CA34172-104 HARLECO Eosin Y Solution, Aqueous, 1% PDC CA15204-258L Eosin Stain Solution, 5% 500 ml CAR03040-74 HARLECO Schiff Reagent 500 ml CA34172-066 HARLECO Schiff Reagent, Hotchkiss/McManus 500 ml CA15204-138 HARLECO Gomori Trichrome 500 ml CA15204-278 Formalin 10% W/V, Neutral Buffered HARLECO Formalin, Neutral Buffered 10% 200 L CAR04586-76 CAR04586-82 CAR04586-86 CAR04586-90 CA34172-118 CA34172-120 Formalin 10%, Neutral Buffered CA34172-118 Formalin Neutral 10%, Carbonate Buffered CAR03379-82 CAR03379-86 H + E Stain - Liver tissue stained with eosin Y solution HARLECO Formalin, Carsons 15204-198 SAF Fixative (Acetate Buffered Formalin) 200 L CAR04650-90 SAF Fixative with Triton (Acetate Buffered Formalin) 200 L CAR04561-90 Formaldehyde Kit Concentrates to make NBF CAR04669-01 HARLECO Bouin Fixation Fluid CA15204-240 CA15204-295 HARLECO Fixative, Haupts 500 ml CA15204-244 HARLECO Blueing Reagent CA34172-018 Mollifex Tissue Softener 500 ml CA34172-108 HARLECO Acid Alcohol, 70% (3% HCl) CA15204-266 HARLECO Alcohol, 70% CA34172-000 HARLECO Krystalon Mounting Medium 300 ml 500 ml CA34172-104 CA34172-034 3

Microbiology Reagents for standard stains and reliable results with manual or automated staining HARLECO bacteriological standard stains for Gram and TB detection offer easy application and reproducible, stable results. The staining solutions can be used for manual as well as for automated staining procedures in the Midas III automated stainer. Product Size Catalog No. HARLECO Gram Staining Set, 4 x 500 ml 1 PAC CA15204-197 HARLECO Crystal Violet, Gram Solution HARLECO Safranin Solution, Gram 1 PAC 1 PAC HARLECO Iodine Solution, Gram (Stabilized) 1 PAC HARLECO Decolorizer, Gram Solution 1 PAC CA15204-189 CA15204-288 CAD65092B-95 CA15204-286 CA15204-193 CA15204-284 CA15204-195 CA15204-282 HARLECO Iodine Lugol's 500 ml CA15204-148 Safranin Solution, 1% 500 ml CAR03131-74 HARLECO Fuchsin, Basic Solution CA15204-260 HARLECO Fuchsin, Carbol Solution, Ziehl-Neelson CA15204-136 HARLECO Fuchsin, Carbol Solution, Kinyoun CA15204-262 Methylene Blue Stain Solution, Loeffler`s 500 ml CAR03101-74 HARLECO Methylene Blue, Loeffler's CA15204-202 Nigrosine Solution, Aqueous 100 ml CAR03418-70L Lactophenol Solution 500 ml CAR03266-74 Lactophenol, Cotton Blue 100 ml CAR03465-70 Gram-color staining Gram-positive coccus 4

Midas III Automated Slide Stainer The Midas III Automated Stainer is designed to work with HARLECO stains and is flexible enough to adapt to your current staining protocols. With a small footprint to allow use on the smallest benchtops, the Midas III Automated Stainer yields staining results that are more reproducible than manual staining. The six station unit holds either 20 or 30 slides. Up to 10 programs may be introduced, with each program comprised of as many as 29 steps. Save up to 50% on stain consumption as compared versus flood or spray techniques. Product Size Catalog No. Midas III Stainer CA15204-300 20 position slide carrier each CA64052-94 30 position slide carrier each CA64052E-94 Water Bath each CA64000WB-94 Water Bath Filters Package of two 25445-016 Vessels & Lids White Package of Eight CAD64054W95 Operators Manual CA64000OP-94 Service Manual CA64000SM-94 Multi-terge detergent 4L CA34171-010 HARLECO Water, distilled ASTM type II 4L CA34172-074 CA34172-073 Midas III Automated Slide Stainer 5

ca.vwr.com/milliporesigma 2017 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. MilliporeSigma, the vibrant M, Sigma-Aldrich, Harleco and Midas are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. Mollifex and Multi-terge are trademarks of American Type Culture Collection. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources. Lit. No. 03918_CAN CM-17-04-0096 11/2017 6 1117 Lit. No. 130032WREV