ABSENCE OF CIRCULATION OF BLUETONGUE VIRUS SEROTYPE 8 IN CAMPO DE GIBRALTAR LVU SINCE NOVEMBER 2010

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ABSENCE OF CIRCULATION OF BLUETONGUE VIRUS SEROTYPE 8 IN CAMPO DE GIBRALTAR LVU SINCE NOVEMBER 21 SCoFCAH 15th January 213

BTV-8 8 EPIDEMIOLOGICAL EVOLUTION IN SPAIN First incursion: January 28 Origin: trade movements Early detection and implementation of proper measures led to a quick control, spatially and temporarily, of the problem: Only 36 outbreaks (between 28 and 21) location of the outbreaks restricted to three specific areas of Spain (Cantabria, Galicia and South of Andalucía)

BTV-8 8 SPAIN 28 DATE PROVINCE LVU OUTBREAKS January Cantabria Solares 12 October- December Málaga Cartama 16 November Lugo Terra Cha 1 TOTAL 29

BTV-8 8 SPAIN 29 DATE PROVINCE LVU OUTBREAKS January-February Málaga Málaga Cartama (Guadalhorce Oriental) Málaga (Guadalhorce Occidental) 3 2 March Cádiz Campo de Gibraltar 1 TOTAL 6

BTV-8 8 SPAIN 21 DATE PROVINCE LVU OUTBREAKS November Cádiz Campo de Gibraltar 1 TOTAL 1

Considering: The favorable epidemiological evolution The absence of BTV-8 circulation since February 29 in the Spanish Mainland (but Campo de Gibraltar LVU) Rapport presented at 7-8 Mai 212 Scofcah: BTV-8 freedom declaration in Spanish mainland but Campo de Gibraltar LVU Not possible to declare freedom in Campo de Gibraltar LVU until January 213

BT MONITORING, SURVEILLANCE AND CONTROL PROGRAM IN SPAIN 21-212 212 Serologic and virologic surveillance: Sentinel animals over 4 month old (cattle, sheep, goats) Not vaccinated, seronegative Prevalence 2%, 95% IC: 149 samples per province 6 holdings per province 1 month of permanence in the province Autonomous Region Minimum samples (1) Bovine BTV-8 positive holdings Bovine BTV-8 positive animals Ovine BTV-8 positive holdings Ovine BTV-8 positive animals 21 74,833 1 2 211 74,833 212 49,434 TOTAL 199,1 1* 2 (1): in accordance to the minimum number of samples stablished in the Bluetongue National Surveillance Program *Corresponding to the 21 single outbreak of BTV-8 in November (Campo de Gibraltar)

OUTCOME OF BTV-8 8 SURVEILLANCE PROGRAM IN CAMPO DE GIBRALTAR LVU DOMESTIC ANIMALS SURVEILLANCE PROGRAM IN CAMPO DE GIBRALTAR LVU Year Serology test (1) Virology test (2) Test total Bovine BTV-8 positive holdings Bovine BTV-8 positive animals Ovinecaprine BTV- 8 positive holdings Ovinecaprine BTV- 8 positive animals 21 32 346 648 1 2 211 47 47 814 212 456 53 959 TOTAL 1,165 1,256 2,421 1* 2 (1): total number of serologic tests performed (2): total number of agent identification tests performed * Corresponding to the 21 single outbreak of BTV-8 in November WILD ANIMALS SURVEILLANCE PROGRAM IN CAMPO DE GIBRALTAR LVU YEAR ELISA PCR SN (1) BTV-8 POSITIVE ANIMALS POSITIVE (2) SPECIES DATE PCR BTV-8 POSITIVE ANIMALS 21 71 8 3 Deer Jan-1 211 57 6 212 14 14 TOTAL 142 82 3* (1): SN (seroneutralization test) *The positive animals were deers older than 12 months

BT MONITORING, SURVILLANCE AND CONTROL PROGRAM IN SPAIN 29-212 212 Clinical surveillance To reinforce the compulsory passive clinical surveillance and the serological-virological surveillance. Sheep 15 holdings per Autonomous region per year are randomly selected Entomological surveillance 21: 88 permanent traps 211: 75 permanent traps Since 1/7/212: 28 permanent traps Vaccination program compulsory until 1/7/211

BTV-8 8 SITUATION IN NEARBY COUNTRIES

CONCLUSIONS No outbreaks of BTV-8 in Campo de Gibraltar LVU since November 21 European Countries that has been affected by BTV-8, with some exceptions, are free Countries more geographically close to Spain: Portugal, France and Morocco are free of BTV-8. The monitoring and surveillance program implemented in Spain observes the requirements set out in Annex I of Regulation 1266/27 as regards the monitoring in order to provide the Commission with substantiated information demonstrating the absence of bluetongue virus circulation in an epidemiological relevant geographical area during a period of two years Program s results in the last 2 years (including 3 full vector activity period): there have no been BTV-8 circulation in Campo de Gibraltar LVU Spain is in position to declare BTV-8 freedom in Campo de Gibraltar LVU

PROPOSED RESTRICTED ZONES