ABSENCE OF CIRCULATION OF BLUETONGUE VIRUS SEROTYPE 8 IN THE SPANISH MAINLAND SINCE FEBRUARY 2009

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ABSENCE OF CIRCULATION OF BLUETONGUE VIRUS SEROTYPE 8 IN THE SPANISH MAINLAND SINCE FEBRUARY 2009 SCoFCAH 7-88 May 2012

BTV-8 8 EPIDEMIOLOGICAL EVOLUTION IN SPAIN First incursion: January 2008 Origin: trade movements Early detection and implementation of proper measures led to a quick control, spatially and temporarily, of the problem: Only 36 outbreaks (between 2008 and 2010) location of the outbreaks restricted to three specific areas of Spain (Cantabria, Galicia and South of Andalucía)

BTV-8 8 SPAIN 2008 DATE PROVINCE LVU OUTBREAKS January Cantabria Solares 12 October- December Málaga Cartama 16 November Lugo Terra Cha 1 TOTAL 29

BTV-8 8 SPAIN 2009 DATE PROVINCE LVU OUTBREAKS January-February Málaga Málaga Cartama (Guadalhorce Oriental) Málaga (Guadalhorce Occidental) March Cádiz Campo de Gibraltar 1 TOTAL 6 3 2

BTV-8 8 SPAIN 2010 DATE PROVINCE LVU OUTBREAKS November Cádiz Campo de Gibraltar 1 TOTAL 1

BT MONITORING, SURVILLANCE AND CONTROL PROGRAM IN SPAIN 1.Surveillance program: 1.Serologic and virologic surveillance 2.Clinical surveillance 3.Entomological surveillance 2.Vaccination program 3.Control of movements

BT MONITORING, SURVILLANCE AND CONTROL PROGRAM IN SPAIN 2009-2012 2012 Serologic and virologic surveillance: Sentinel animals over 4 month old (cattle, sheep, goats) Not vaccinated, seronegative Prevalence 2%, 95% IC: 149 samples per province 6 holdings per province Risk areas: 17 samples/year No risk areas: 9 samples/year

OUTCOME OF BTV-8 8 SURVEILLANCE PROGRAM Year Minimum samples (1) ELISA (2) PCR (3) DOMESTIC ANIMALS SURVEILLANCE OUTCOME BTV-8 positive holdings BTV-8 positive animals Autonomo us region 2009 74.833 149.718 81.956 6 20 Andalucía Province Málaga Cádiz LVU Guadalhorce Oriental and Occidental Campo de Gibraltar 2010 74.833 93.356 57.555 1 2 Andalucía Cádiz Campo de Gibraltar 2011 74.833 93.356 47.535 0 0 (1): in accordance to the minimum number of samples stablished in the Bluetongue National Surveillance Program (2): total number of serologic tests performed (3): total number of agent identification tests performed WILD ANIMALS SURVEILLANCE OUTCOME IN ANDALUCÍA Year PCR SERONEUT RALIZATIO N (ST) ST POSITIVE ANIMALS PROVINCE SERONEUTRALIZATION LVU SPECIES DATE PCR PCR POSITIVE ANIMALS 2010 263 429 3 Cádiz Campo Gibraltar Deer January 2010 0 2011 394 426 0 0 Hitherto, as regards 2012, all samples tested in domestic and wildlife have resulted negative to serotype 8 of the Bluetongue virus

BT MONITORING, SURVILLANCE AND CONTROL PROGRAM IN SPAIN 2009-2012 2012 Clinical surveillance To reinforce the serological-virological surveillance and to detect new BTVs circulating in an area Sheep 150 holdings per Autonomous region per year are randomly selected Entomological surveillance 2009: 95 permanent traps 2010: 88 permanent traps 2011: 75 permanent traps

BT MONITORING, SURVILLANCE AND CONTROL PROGRAM IN SPAIN 2009-2012 2012 Vaccination program: High level of immunisation by: Massive compulsory vaccination program: coverage over 80% of the animals Natural immunity Absence of BTV-8 circulation since 2010 Limited circulation of BTV-1 and BTV-4 during the current season

BTV-8 8 SITUATION IN NEARBY COUNTRIES COUNTRY STATUS LAST OUTBREAK Northern EU free France not free December 2009 Portugal free Morocco free

CONCLUSIONS No outbreaks of BTV-8 in Spain (with the exception of Campo de Gibraltar LVU) since February 2009 European Countries that has been affected by BTV-8, with some exceptions, are free Countries more geographically close to Spain: Portugal and Morocco are free of BTV-8. France no outbreaks since December 2009 The monitoring and surveillance program implemented in Spain observes the requirements set out in points 1.3 and 1.1.2.1 of Annex I of Regulation 1266/2007 as regards the monitoring in order to provide the Commission with substantiated information demonstrating the absence of bluetongue virus circulation in an epidemiological relevant geographical area during a period of two years Program s results in the last 3 years (including two full vector activity period): there have no been BTV-8 circulation in Spain (with the exception of Campo de Gibraltar LVU) since February 2009 Spain is in position to declare BTV-8 freedom in the whole territory with the exception of Campo de Gibraltar LVU

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