1 Electronic supplements for 2 Invaders versus endemics: alien gastropod species in ancient Lake Ohrid 3 Hydrobiologia 4 Christian Albrecht Kirstin Föller Catharina Clewing Torsten Hauffe Thomas Wilke 5 6 Department of Animal Ecology and Systematics, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany 7 E-mail: Christian.Albrecht@allzool.bio.uni-giessen.de 8 9 10 11 12 13 Electronic Supplement Table 1 Newly sequenced (partial cytochrome c oxidase I) specimens of Physa acuta, Ferrissia fragilis, and Ferrissia rivularis. All haplotypes and the corresponding locality information, voucher information (University of Giessen Systematics and Biodiversity Collection UGSB) and NCBI GenBank accession numbers are provided. Superscript numbers at locality codes refer to tip lables of the phylogenetic trees. Haplotype Species Locality code Locality Latitude Longitude Specimen voucher no. 1 COI GenBank accession no. HTP 1 Physa acuta Channel Lysimachia Greece, West Greece, channel between Lake Trichonis 38.55092 N 21.44083 E UGSB 12284 KF737940 & Lake Lysimachia UGSB 12285 KF737941 Inflow Lysimachia Greece, West Greece, inflow to Lake Lysimachia 38.54749 N 21.37608 E UGSB 12286 KF737949 Inflow Pamvotis Greece, Epirus, Inflow to Lake Pamvotis 39.68330 N 20.87803 E UGSB 12287 KF737948 UGSB 12288 KF737947 Lake Kerkini Greece, Central Macedonia, Lake Kerkini (artificial lake) 41.13943 N 23.22577 E UGSB 12290 KF737925 Lake Lysimachia 1 Greece, West Greece, Lake Lysimachia 38.54117 N 21.38730 E UGSB 12291 KF737927 Lake Lysimachia 2 Greece, West Greece, Lake Lysimachia 38.55286 N 21.38009 E UGSB 12292 KF737942 Lake Ohrid 1 Macedonia, Lake Ohrid (Ohrid Bay) 41.10558 N 20.80746 E UGSB 12294 KF737928 Lake Pamvotis 1 Greece, Epirus, Lake Pamvotis 39.67405 N 20.91468 E UGSB 12295 KF737930 UGSB 12296 KF737931 Lake Pamvotis 2 Greece, Epirus, Lake Pamvotis 39.68318 N 20.87761 E UGSB 12297 KF737938 Lake Vegoritis 1 Greece, Central Macedonia, Lake Vegoritis 40.72947 N 21.80602 E UGSB 12298 KF737921 Lake Vegoritis 2 Greece, Central Macedonia, Lake Vegoritis 40.78619 N 21.81685 E UGSB 12299 KF737946 (peninsula opposite Arnissa) UGSB 12300 KF737945 Lake Vegoritis 3 Greece, Central Macedonia, Lake Vegoritis 40.78198 N 21.81571 E UGSB 12301 KF737944 (peninsula opposite Arnissa) UGSB 12302 KF737943 Lake Vegoritis 4 Greece, West Macedonia, Lake Vegoritis 40.72040 N 21.75136 E UGSB 12303 KF737951 River Strymonas 1 Greece, Central Macedonia, River Strymonas 40.81136 N 23.82929 E UGSB 12304 KF737932 UGSB 12305 KF737933 HTP 2 Physa acuta Lake Trichonis Greece, West Greece, Lake Trichonis 38.55133 N 21.53817 E UGSB 12306 KF737929 HTP 3 Physa acuta Lake Kastraki Greece, West Greece, Lake Kastraki (artificial lake) 38.82002 N 21.33194 E UGSB 12307 KF737952 Lake Lysimachia 2 Greece, West Greece, Lake Lysimachia 38.55286 N 21.38009 E UGSB 12293 KF737950 HTP 4 Physa acuta Lake Dojran 1 Macedonia, Stari Dojran, Lake Dojran 41.18786 N 22.72310 E UGSB 12308 KF737939 River Gallikos Greece, Central Macedonia, River Gallikos 40.79233 N 22.84481 E UGSB 12309 KF737936 UGSB 12310 KF737937
14 HTP 5 Physa acuta Lake Dojran 2 Macedonia, Novi Dojran, Lake Dojran 41.21457 N 22.70995 E UGSB 12311 KF737926 Lake Kerkini Greece, Central Macedonia, Lake Kerkini (artificial lake) 41.13943 N 23.22577 E UGSB 12289 KF737924 Lake Volvi Greece, Central Macedonia, pool next to Lake Volvi 40.65673 N 23.42553 E UGSB 12312 KF737922 UGSB 12313 KF737923 River Strymonas 2 Greece, Central Macedonia, River Strymonas 41.05587 N 23.34235 E UGSB 12314 KF737934 UGSB 12315 KF737935 HTF 1 Ferrissia fragilis Lake Kalodiki Greece, Epirus, Lake Kalodiki 39.31897 N 20.44967 E UGSB 12316 KF737916 Lake Lysimachia 3 Greece, West Greece, Lake Lysimachia 38.56169 N 21.35286 E UGSB 2534 KF737918 Lake Mergozzo Italy, Verbano-Cusio-Ossola, Lake Mergozzo 45.95554 N 08.46550 E UGSB 12602 KF737919 (type locality of F. wautieri) UGSB 12603 KF737920 Lake Ohrid 2 Albania, Pogradec District, Lake Ohrid (Lin peninsula) 41.05138 N 20.64190 E UGSB 12317 KF737917 HTF 2 Ferrissia rivularis Monocacy River USA, Maryland, Frederick Co., creek near to River Monocacy 39.37003 N 77.38708 W UGSB 12318 KF737913 HTF 3 Ferrissia rivularis McClane Creek USA, Washington State, Thurston Co., McClane creek wetlands 47.00175 N 123.00602 W UGSB 12319 KF737914 HTF 4 Ferrissia rivularis Newaukum River USA, Washington State, Lewis Co., North Fork River Newaukum 46.60861 N 122.85083 W UGSB 12320 KF737915 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 2
33 34 35 36 37 Electronic Supplement Table 2 Known occurrences of Physa acuta in natural and artificial lakes of the Balkans. The first record and the current status (latest record) are listed. Only records of live populations are considered. Presence with unknown first record is marked with a?.?? marks an unclear situation. Voucher material obtained during field work from 2003 to 2012 is deposited at the University of Giessen Systematics and Biodiversity collection (UGSB). 38 Lake First record Data source Current status Data source Amvrakia - - Not found this study Cheimaditidas? Reischütz, pers. 6/2008 this study Dojran 1985 Stanković (1985) 9/2007 this study Kastorias? Reischütz, pers. 5/2005 this study Kerkini 9/2007 this study Koronia? Reischütz, pers. -, almost this study completely dried out in 9/2007 9/2007 this study Lysimachia 8/2002 Reischütz & Mikri Prespa 7/2008 Reischütz, pers. 7/2008 Reischütz, pers. Ohrid 2009 Albrecht et al. 2011 this study (2009b) Ozeros? Reischütz, pers. Not found this study Pamvotis 2002 Reischütz & 6/2013 Fehér, pers. Reischütz (2003 Paralimni 7/2004 Reischütz &?? Vardala- Theodorou et al. (2006) Petreo - - Not found this study Pikrolimni - - -, completely this study dried out in 9/2007 Prespa 8/2004; shells Reischütz, pers. - this study only Skutari 2002 Dhora (2002) 6/2012 Fehér, pers. Trichonis 8/2002 Reischütz & 9/2007 this study Vegoritis 5/2005 this study 6/2008 this study 3
Volvi? Reischütz, pers. 9/2007 this study Yliki 7/2004 Reischütz &?? Vardala- Theodorou et al. (2006) Zazari - - Not found this study 39 40 41 42 43 44 45 46 Electronic Supplement Table 3 Known occurrences of Ferrissia fragilis in natural and artificial lakes of the Balkans. The first record and the current status (latest record) are listed. Only records of live populations are considered. Note that all published records of Ferrissia are regarded as Ferrissia fragilis, according to the results of the current study. Voucher material obtained during field work from 2003 to 2012 is deposited at the University of Giessen Systematics and Biodiversity collection (UGSB). 47 Lake First record Data source Current status Data source Amvrakia - - Not found this study Cheimaditidas - - Not found this study Dojran - - 9/2009 this study Kastorias 6/2013 Fehér, pers. 6/2013 Fehér, pers. Kerkini - - Not found this study Koronia - - -, almost this study completely dried out in 9/2007 Lysimachia 8/2002 Reischütz & 9/2007 this study Mikri Prespa - - Not found this study Ohrid 2010 2010 2010 this study Ozeros - - Not found this study Pamvotis 2002 Reischütz & Reischütz (2002) Paralimni 7/2004 Reischütz & 2007 Frogley & Preece (2007) 7/2004 Reischütz & 4
Petreo - - Not found this study Pikrolimni - - -, completely this study dried out in 9/2007 Prespa 10/2005 Albrecht et al. (2012) 2005 Albrecht et al. (2012) Skutari 2006 this study?? Glöer & Pešić (2013) Trichonis 8/2002, shells only Reischütz & 9/2007 Albrecht et al. (2009) Vegoritis 1962 Schütt (1985) 6/2008 this study Volvi - - Not found this study Yliki - Reischütz & - Reischütz & Zazari - - Not found this study 48 49 50 51 52 53 54 55 56 57 5