www.academicjournals.com
OPEN ACCESS Asian Journal of Animal and Veterinary Advances ISSN 1683-9919 DOI: 10.3923/ajava.2017.227.238 Research Article DNA Barcoding of Cuscuses (Marsupialia: Phalangeridae) from Maluku and Papua 1 Rony Marsyal Kunda, 2 Niken Satuti Nur Handayani, 3 Hery Wijayanto and 4 Rini Widayanti 1 Department of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 2 Department of Genetic, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 3 Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 4 Department of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
E E E E E 1000 bp 919 bp 919 bp 500 bp
61 47 90 94 82 97 93 99 Cuscus (Lakor island brown linear black) Cuscus (North Seram island) whole white Cuscus (Geser island) brown linear black 66 Cuscus (Allang Ambon island) whole white Cuscus (Larike Ambon island) brown linear black Cuscus (Geyser island) whole white 100 Clade A1 Cuscus (Hatu Ambon island) brown linear black Cuscus (Kariu Haruku island) whole white Cuscus(Gorom island) whole white Cuscus (Kariu Haruku island) brown linear black 66 Cuscus (Kariu Haruku island) brown linear black 2 96 Cuscus (Wanggar Nabire island) brown linear black Cuscus (Moor Nabire island) brown linear black Clade A2 100 Cuscus (Wanggar Nabire island) grey linear black 78 Cuscus (Yaro Nabire island) brown linear black Phalanger vestitus Phalanger gymnotis Cuscus (Nabire island) spotted 1 99 Cuscus (Nabire island) spotted 2 Cuscus (Nabire island) spotted 3 Cuscus (Nabire island) spotted 4 Cuscus (Sentani Jayapura island) spotted 100 94 Cuscus (Halmahera island) spotted Clade B2' Spilocuscus maculatus Cuscus (South Seram island) spotted 78 Cuscus (Soya Ambon island) spotted Clade B1 99 Cuscus (Allang Ambon island) stein Cuscus (Manipa island) stein Ailurops ursinus Strigocuscus celebensis Trichosurus vulpecula Wyulda squamicaudata Out-group Vombatus ursinus Lagostrophus fasciatus Petrogale brachyotis 0.02
ATG ACT CAC CAA ACA CAC GCA TAT CAC ATA GTT AAC CCA AGC CCA [ 45].....C..........................C............ [ 45].....C..........................C............ [ 45].....C..........................C............ [ 45].....C..........................C............ [ 45].....A........G.............................. [ 45].....A........G.............................. [ 45].....C........G..T........................... [ 45].....A........G.............................. [ 45] ATG ACC CAC CAA ACG CAC GCT TAT CAC ATA GTT AAT CCA AGT CCA [ 45] TGA CCA CTA ACA GGA GCC CTA TCA GCC CTA CTG CTT ACA TCA GGT [ 90]...... T.........................A..C........C [ 90]................................A..C........C [ 90]................................A..C........C [ 90]...... T.........................A..C........C [ 90] TGG CCA TTA ACA GGA GCT CTA TCA GCC CTA CTA CTC ACA TCA GGC [ 90] CTC ATC ATA TGG TTC CAC TTC AAC TCC CCA CTC TTA GTA CTA ATC [135]...........A........T...... T.C A.T....C....... [135]...........A........T...... T.C A.T....C....... [135]...........A........T...... T.C A.T....C....... [135]...........A........T...... T.C A.T....C....... [135] CTC ATC ATA TGA TTC CAC TTC AAC TCC TCT ACA TTA GTA CTA ATC [135] GCA TTC TTT TTT CTA GGG TTT TTC TGA GCC TTT TAT CAC TCG AGC [315].T......C....................T..C........A... [315].T......C....................T..C........A... [315].T......C....................T..C........A... [315].T......C....................T..C........A... [315] GTA TTC TTC TTC CTA GGA TTC TTC TGA GCC TTC TAC CAT TCA AGC [315]
Cus cus (Lakor Island) brown linear black CTA GCC CCA ACC CAT GAA TTA GGA GGC TGC TGA CCC CCT ACC GGA [360]...................................A..C...... [360].................. C................A..C...... [360].................. C................A..C...... [360]...................................A..C...... [360] CTA GCC CCA ACC CAC GAA CTA GGA GGC TGT TGA CCA CCA ACC GGA [360] ATC CAC CCA TTA AAC CCA CTC GAA GTC CCA CTA CTC AAC ACA TCC [405]..........................T...........T...... [405]..........................T...........T...... [405]..........................T...........T...... [405]..........................T...........T...... [405] ATT CAT CCA TTA AAT CCA CTT GAA GTT CCA CTA CTT AAC ACA TCA [405] ATT CTA CTA GCC TCC GGA GTA TCA ATC ACA TGA GCC CAC CAC AGT [450]...... T.G.....T..G........................... [450]...... T.G.....T..G........................... [450]...... T.G.....T..G........................... [450]...... T.G.....T..G........................... [450] ATC TTA TTG GCT TCC GGA GTA TCA ATT ACA TGG GCT CAC CAC AGC [450] CTA ATA GAA GGC AGT CGA GAA CAA ATA ATT CAA GCA CTA TCA ATT [495].............AC... A.........................C [495].....G.......AC... A.........................C [495].....G.......AC... A.........................C [495].............AC... A.........................C [495] CTA ATA GAA GGA AAC CGA AAA CAA ATA ATT CAA GCA CTA TCA ATT [495]
TAC TAC GAA TCA TCC TTT ACA ATC TCA GAC GGC ATC TAC GGA TCC [585]..T..T...........C...........T..T.....T..G..A [585]..T..T...........C...........T..T.....T..G..A [585]..T..T...........C...........T..T.....T..G..A [585]..T..T...........C...........T..T.....T..G..A [585] TAC TAT GAA TCA TCT TTC ACC ATT TCA GAC GGC ATC TAC GGA TCA [585] CAC TTC ACA TCC ACT CAC CAC TTT GGA TTC GAA GCA GCT GCC TGA [720]...........A........T........................ [720]...........A........T........................ [720]...........A........T........................ [720]...........A........T........................ [720] CAC TTT ACA TCC ACT CAC CAC TTC GGA TTT GAA GCA GCT GCC TGA [720] TAT TGA CAC TTC GTA GAT GTG GTA TGA CTC TTC TTA TAC GTA TCA [765]...........T........A............ C........... [765]...........T........A............ C........... [765]...........T........A............ C........... [765]...........T........A............ C........... [765] TAC TGA CAC TTC GTG GAC GTA GTA TGA CTC TTC CTA TAT GTG TCA [765] ATT TAC TGA TGA GGT TCG [783]..............C..A [783]..............C..A [783]..............C..A [783]..............C..A [783] ATT TAC TGA TGA GGC TCA [783]
Spilocuscus maculatus (Genebank) Cuscus (Nabire Island) spotted 1 Cuscus (Nabire Island) spotted 2 Cuscus (Nabire Island) spotted 3 Cuscus (Nabire Island) spotted 4 Cuscus (Sentani Jayapura Island) spotted Cuscus (Halmahera Island) spotted Cuscus (South Seram Island) spotted Cuscus (Soya Ambon Island) spotted Cuscus (Allang Ambon Island) stein Cuscus (Manipa Island) stein Spilocuscus maculatus (Genebank) Cuscus (Nabire Island) spotted 1 Cuscus (Nabire Island) spotted 2 Cuscus (Nabire Island) spotted 3 Cuscus (Nabire Island) spotted 4 Cuscus (Sentani Jayapura Island) spotted Cuscus (Halmahera Island) spotted Cuscus (South Seram Island) spotted Cuscus (Soya Ambon Island) spotted Cuscus (Allang Ambon Island) stein Cuscus (Manipa Island) stein Spilocuscus maculatus (Genebank) Cuscus (Nabire Island) spotted 1 Cuscus (Nabire Island) spotted 2 Cuscus (Nabire Island) spotted 3 Cuscus (Nabire Island) spotted 4 Cuscus (Sentani Jayapura Island) spotted Cuscus (Halmahera Island) spotted Cuscus (South Seram Island) spotted Cuscus (Soya Ambon Island) spotted Cuscus (Allang Ambon Island) stein Cuscus (Manipa Island) stein Spilocuscus maculatus (Genebank) Cuscus (Nabire Island) spotted 1 Cuscus (Nabire Island) spotted 2 Cuscus (Nabire Island) spotted 3 Cuscus (Nabire Island) spotted 4 Cuscus (Sentani Jayapura Island) spotted Cuscus (Halmahera Island) spotted Cuscus (South Seram Island) spotted Cuscus (Soya Ambon Island) spotted Cuscus (Allang Ambon Island) stein Cuscus (Manipa Island) stein ATG ACT CAC CAA ACA CAT GCA TAT CAC ATA GTT AAC CCA AGC CCA [ 45]................................C............ [ 45]................................C............ [ 45]................................C............ [ 45]................................C............ [ 45]............................................. [ 45]............................................. [ 45].................C........................... [ 45].................C........................... [ 45].................C........................... [ 45].................C........................... [ 45] CTA ATC ATA TGA TTC CAC TTT AAC TCC TCC ATC CTG GCA TTA ACT [135]..........................T........A......... [135]..........................T........A......... [135]..........................T........A......... [135]..........................T........A......... [135]..........................T.................. [135]..........................T.................. [135]............................................. [135]............................................. [135]............................................. [135]............................................. [135] CTA GCA CCA ACC CAC GAA CTA GGA GGC TGT TGA CCA CCT ACC GGA [360]............................................. [360]............................................. [360]............................................. [360]............................................. [360].............................C............... [360].............................C............... [360]......................................C...... [360]......................................C...... [360]......................................C...... [360]......................................C...... [360] ATC CTA TTA GCT TCT GGT GTA TCA ATC ACA TGA GCC CAC CAT AGC [450]..T........C................................. [450]..T........C................................. [450]..T........C................................. [450]..T........C................................. [450]............................................. [450]............................................. [450]..............C.............................. [450]..............C.............................. [450]..............C.............................. [450]..............C.............................. [450]