User friendly microbial detection! Sanita-kun R =The ready-to-use cultural medium sheet for the microbial detection = Chisso Corporation e-mail sanita-kun@chisso.co.jp web http:// ://www.chisso.co.jp/sanita/
What is the SANITAKUN? SANITAKUN is our original product Sheet Medium. SANITAKUN is a ready-to use cultural medium sheet equipped with non-woven fabric. SANITAKUN provides easy and accurate detection of microorganisms to customers as an alternative to agar plate.
The structure of SANITAKUN Transparent cover film (oxygen permeability) Fabric pad Non-woven fabric Water-soluble high polymer nutrients chromogen inhibitor Adhesive film waterproof sheet Apply 1ml sample solution Applied sample solution diffuses throughout the entire pad in a few seconds. incubate High viscous solution dissolves, it pushes up microorganism to the fabric surface. The Microorganism grows and forms colony on the surface and it colors by chromogen.
SANITAKUN line-ups SANITAKUN Chromogenic principal incubation Aerobic Count Coliform Yeast & Mold Chromogen(TTC) colors red by a microbial metabolism. Chromogen colors blue or blue-green by -galactosidase that coliform generates. Chromogen colors blue or indigo blue by esterase that yeast or mold generates. 35 8hr 35 hr 5 8hr Staph. aureus E.coli/Coliform Salmonella Chromogen colors black spot and blue halo around the spot. Chromogen colors blue or blue-green by -galactosidase that coliform generates. Chromogen dyes indigo blue by -glucuronidase that E.coli generates. Chromogen colors blue by -galactosidase that salmonella generates. (Chromogen dyes indigo blue by -galactosidase that Coliform generates.) 35 hr 35 hr 35 hr
SANITAKUN chromogenic characteristic Aerobic Count Coliform Yeast & Mold Staph. aureus E.coli/Coliform Salmonella
Concept of SANITAKUN User friendly Care free Reliable 1) Easy to use ) Multi applications 3) Accurate & Quantitative ) Reliability 5) Ready-to-use & Time saving 6) Space saving & Small volume of waste 7) Long validity
Characteristics 1: Easy to use Direct stamping Foods or swabbing sample solution Incubation
Characteristics : Multi applications Swabbing Water filtrate Direct stamping Swabbing test used swabbing kit Fall out test Food sample test
Characteristics 3 : Accurate & Quantitative SANITAKUN Aerobic Count 8 Incubation for 8h y = 1.0338x - 0.767 Sanita-kun Aerobic Count(logcfu/mL) 6 0 0 6 8 Plate Count Agar(logcfu/mL)
SANITAKUN Aerobic count Comparison between SANITAKUN Aerobic Count and agar plate / AOAC Official Method 966.3 8 7 y = 0.986x - 0.0197 R = 0.99 Sanita-kun (logcfu/g) 6 5 3 1 1 3 5 6 7 8 Plate Count Agar (logcfu/g)
SANITAKUN Aerobic Count (X;log agar plate,y;log SANITAKUN) 35,days Fresh produce (n=5) 8 6 y = 1.083x - 0.387 0 0 6 8 8 6 0 Sea foods (n=77) y = 1.039x - 0.873 0 6 8 8 Candy (n=83) Dairy (n=5) Grain(n=16) 6 8 Meat(n=9) 6 6 y = 1.00x + 0.0 0 0 6 8 0 0 6 0 0 6 8 Bean(n=6) Seaweed(n= 9) 8 6 Daily dish(n=17) 6 Egg(n=) 0 y = 1.1x - 0.385 0 0 0 y = 1.019x - 0.08 0 6 8 0 y = 1.197x - 0.519 0 6
SANITA-KUN Coliform Comparison between SANITAKUN and agar SanitaKun Coliform (logcfu/g) SanitaKun Coliform (logcfu/g) Desoxycholate agar(logcfu/g) X-Gal agar(logcfu/g)
SANITA-KUN Yeast & Mold (Rapid Type) Comparison between SANITAKUN and PDagar
Characteristics :Reliability The international certification SANITAKUN Aerobic Count, Coliform were approved by AOAC Research Institute as the Performance Tested Method. SANITAKUN E.Coli/Coliform was approved by AOAC Research Institute as the Performance Test Method on this July We will try to apply a validation such a AOAC PTM approval about SANITAKUN other item, then too. The official recognition in Japan SANITAKUN was appeared on the Food Sanitation Inspection Guide Line which official guide line of Japan. ( Aerobic Count, Coliform, Staph. aureus, E.coli/Coliform )
Characteristics 5: Ready-to-use & Time saving Comparison of preparation time between SANITAKUN and agar plate (100 tests) Time 0 1 3 8 hours Agar Start measuring autoclaving Sample application cooling incubation Start Sample application SANITAKUN We can save 3 hours using SANITAKUN
Characteristics 6: Space saving Small volume of waste 10 agar plates Space saving 10 SANITAKUNs sheets to agar plates Small volume of waste to agar plates 0 autoclaved plates. litters 00 autoclaved SANITAKUNs sheets
Characteristics 7: Long validity Sanita-kun Aerobic count Coliform Yeast & Mold Staph. aureus Salmonella validity (M) 36 E.coli/Coliform 36 Validity from manufacturing date in a stock condition of tight sealing in a refrigerator. 36
SANITA-KUN s field Food processing Convenience store s vender Healthy foods Poultry & Meat Industries Fish Industries Hotels & Restaurants and Others Cosmetic Water supply school lunch etc