HERD project: STUDY OF THE MICROBIOLOGICAL FLORA OF MILK AND DAIRY PRODUCTS IN KOSOVO The microbial flora (quality) of (i) milk and (ii)dairy products in Kosovo: With emphasis on the dairy lactic acid bacteria and the two pathogens Listeria monocytogenes and Staphylococcus aureus
Research Education Information Farmers and Company
Institutions and People Involved University of Pristina: Ibrahim Mehmeti, Post. Doc. Skender Muji, Professor Hysen Bytyqi, Professor 2 Master student (right now) Selami Salihu (Master) Ibadete Krasniqi (Master) Imer Haziri (PhD student) 3 Bachelor student (Diploma for Bachelor) Norwegian University of Life Sciences: Ingolf F. Nes, Professor Dzung Bao Diep, Professor Technical Support
EDUCATION
Master student Ibadete Krasniqi (UP- Pristina Kosovo) Selami Salihu (Agriculture University - Tirana Albania)
Bachelor student 1. Remzi Jetullahu 1. Valdet Thaqi 2. Idriz Lalloshi
Work Training at LMG-UMB 1.Imer Haziri (PhD student) 1.Ibadete Krasniqi Master student
Laboratory Manuel Microbiology and food microbiology Agriculture Faculty and Veterinary used for students, farmers and company
RESEARCH
RESULTS I. Isolation and characterization of bacteria in milk obtained from producers
Sampling of milk for bacterial analysis Communities: 10 Farms: 237 Number of samples: 711 (3 tomes from each farm) In December-February (237 samples) In March- April (237 samples) In May-June (237 samples) Bacterial isolates: 5000
Samples collection Sharri Rahovec Skenderaj Lipjanë Podujeva Istogu Skenderaj Istogu Rahoveci Gjakova Theranda Ferizaji Sharri Sharri
Total Viable Count 35 TVC /ml 30 28% complyies with the regulation 25 20 35,29 15 10 5 4,99 6,33 9,42 7,6 6,91 5,48 5,88 5,49 6,47 5,14 0 < 1 100 000 < 100 001 200 000 < 200 001-300 000 < 300 001 400 000 < 400 001 500 000 < 500 001 600 000 < 600 001 700 000 < 700 001 800 000 < 800 001 900 000 < 900 001 1000 000 < 1 000 000...
Analyses 5000 different strains are collected 3000 Analyses in Norway 888 are analyzed for biochemical testes: Growth at different ph (6.0 and 9.2) Growth at different NaCl comcentration (2% and 6.4%) Growth in two different mediums: MRS and GM17 Gas production(homo-fermentative and hetero-fermentative) 888 LAB for bacteriocin production Multi - Locus Sequencing -Typing (MLST)?
Classification Present status of 3000 isolates: Staphylococcus aureus: 235 isolates Listeria spp. 112 isolates Lactic acid bacteria: 2653
Meeting and visits in farms Farms Higher number (more contaminations) Between higher and normal Low number (according to regulation) Milk in company
Higher number of microorganisms Bad hygiene; No pipline desinfection; Hygiene of the farms;
Between extreme and limits
Good quality Good hygiene Good ventilation Pipeline in good conditions
Bacteriocins activity 576 samples are tested; 10 % are positive Indicators: Staphilococcus aureus Listeria innocua Bacillus cerus Lactococcus lactis Lactobacillus sakei
Presentations Week Science in Kosovo Two oral presentations: Ibrahim Mehmeti and Ibadete Krasniqi Conference in AlbShkenca organized in Macedonia Ibadete Krasniqi oral presentation and Poster
Future work
Samples collection company Skenderaj Podujeva Istogu Sharri Rahovec Lipjanë Skenderaj Istogu Rahoveci Gjakova Theranda Ferizaji Sharri Sharri
Analysis of dairy products
Workshops Two workshops next summer organized with farmers and company; Results presentation; Gust from Norway and Kosovo
Equipments
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