Introduction Master Materials and Equipment List Italicized entries indicate items not available from PASCO. The quantity indicated is per student or group. NOTE: Some activities also require protective gear for each student (for example, safety goggles, gloves, apron, or lab coat). Teachers can conduct some lab activities with sensors and probes other than those listed here. For assistance with substituting compatible sensors and probes for a lab activity, contact PASCO Teacher Support (800-772-8700 inside the United States or http://www.pasco.com/support). Act Title Materials and Equipment Part Scientific Inquiry Use a fast response temperature probe to design a simple experiment in which students attempt to slow the cooling rate of the water by adding insulation to the cup. 2 Enzyme Action Use an oxygen gas sensor to understand how optimal environmental conditions, such as temperature, play a key role in enzyme function. 3 Membrane Permeability Use a ph sensor to explore the permeability of a celllike membrane to hydrogen (H + ) and hydroxide (OH ) ions. Observe that not all materials are able to pass through the membrane. PASPORT Fast Response PS-235 Temperature Probe 9-2 oz. cup Hot water Insulating devices readily available in the laboratory (styrofoam, foil, plastic wrap, cloth, wool, packing peanuts) A variety PASPORT Oxygen Gas PS-226 Beaker, -L 2 Beaker, 500-mL Catalase Source/Yeast Suspension 30 ml 500 ml Graduated cylinder, 25-mL Hydrogen Peroxide, 3% 30 ml Ice, crushed or cube L Sampling bottle (provided with the Test tube 2 Tongs 500 ml PASPORT ph * PS-202* 0. M hydrochloric acid (HCl),5 ml 5 ml 0. M sodium hydroxide (NaOH ) 5 ml Beaker, 250-mL Binder clip Dialysis tubing, 5-cm length 2 L Large Base and Support Rod ME-9355 Lugol's iodine 3 ml Magnetic Stirrer and Magnetic Spin SE-7700 Bar Starch solution 50 ml String 50 cm Utility clamp SE-9446 2 Wash bottle
Master Materials and Equipment List Act Title Materials and Equipment Part 4 Organisms and ph Use a ph sensor to determine how effective various substances are at buffering large changes in ph. 5 Osmosis Use a barometer/low pressure sensor to explore the concept of cell membranes and how water and other substances pass through a membrane through the process of osmosis. 6 Plant Respiration and Photosynthesis Use a carbon dioxide gas sensor to understand the comparative concentrations of CO 2 gas for a small plant in darkness and in bright light and what this says about photosynthesis and the CO 2 cycle. 7 Respiration of Germinating Seeds Use a carbon dioxide gas sensor to understand the comparative rates of CO 2 gas production for dry, PASPORT ph * PS-202* Beaker, 50-mL 6 Beaker, 250-mL Erlenmeyer flask -L 2 Graduated cylinder, 0-mL Disposable pipets 2 Detergent solution 5 ml Lemon juice 5 ml L Liver suspension 50 ml Buffer solution 50 ml PASPORT Barometer/low pressure PS-23A PASPORT Extension Cable PS-2500 Beaker, 00-mL 2 Beaker, 400-mL Dialysis tubing,5 cm 2 L Electronic Balance SE-8823 Funnel Graduated cylinder, 0-mL Graduated cylinder, 50-mL Paper towels 3 or 4 Plastic tubing, 5 cm Quick-Release Connector (comes with the Ring Stand with Test Tube or Three ME-9355 Finger Clamp SE-9446 Syrup (maple or corn) 0 ml Thread (or dental floss) to tie dialysis tubing PASPORT Carbon Dioxide Gas PS-20 PASPORT Extension Cable PS-2500 Aluminum foil foot Box or heavy cloth (to cover the bottle) Lamp, 00-watt (or equivalent) Large beaker or aquarium (to keep the light source from heating the sample) Mint plant Sampling Bottle (included with 2 L PASPORT Carbon Dioxide Gas PS-20 PASPORT Extension Cable PS-2500 Beaker, 000-mL Ice, cubed or crushed L 2 PS-2870
Act Title Materials and Equipment Part dormant seeds; for wet, germinating seeds at room temperature; and for wet, cold, germinating seeds. 8 The Role of Buffers in Biological Systems Use a ph sensor to determine which solution is the best buffer. Teacher Information Pea or dry bean seeds 30 Sampling bottle (included with L PASPORT ph * PS-202* Beaker, 250 ml 3 Club soda 200 ml L Graduated cylinder (0 ml) Large Base and Support Rod Magnetic Stirrer and Magnetic Spin SE-7700 Bar Utility Clamp SE-9446 Vinegar, 5% acetic acid 20 ml Wash bottle 9 Acid Rain Use a ph sensor to PASPORT ph * PS-202* determine the effect of M HCl 5 ml several gases that cause -hole rubber stopper for flask acid rain on the ph of water. Beaker, 00-mL Discuss the effect of changes Electronic Balance SE-8823 in the ph of water on the Erlenmeyer flask, 00-mL environment. Flexible tubing to fit glass tubing, 20 cm Glass tubing for rubber stopper Graduated cylinder, 25-mL Graduated pipet and pipet bulb Sodium bicarbonate (NaHCO 3 ) 5 g Sodium bisulfite (NaHSO 3 ) 5 g Sodium nitrite (NaNO 2 ) 5 g Wash bottle containing distilled or deionized water or deionized water 60 ml 0 Cellular Respiration in Yeast Use a dissolved oxygen sensor to measure the dissolved oxygen concentration in yeast solutions in the presence and absence of sugar. Calculate the rate of oxygen consumption of yeast during aerobic cellular respiration at different temperatures. PASPORT Dissolved Oxygen PS-208* * PASPORT Fast Response PS-235 Temperature Activated Yeast Solution 45 ml Beaker, 250-mL 3 L Electronic Balance SE-8823 Graduated cylinder, 25mL Hot Plate SE-8830 Ice bath (-L Beaker filled with ice) Labeling tape roll Marker Stirring rod Sugar 30 g Weighing papers 3 3
Master Materials and Equipment List Act Title Materials and Equipment Part Energy Content of Food Use a temperature sensor to measure the change in temperature of water that is heated by burning samples of food and to compare the energy content of those samples. 2 Exploring Microclimates Use a weather anemometer sensor and current local weather conditions to measure relevant weather conditions at different locations. Determine the impact that a location's environmental conditions have on the microclimate of a given area. PASPORT Temperature * PS-225* PASPORT Extension Cable PS-2500 Aluminum can, 354-mL (2 ounce) 00 ml Electronic Balance SE-8823 Food holder (0 x 0 cm cardboard, aluminum foil, paperclips) Food sample 2 Graduated Cylinder, 00-mL Large Base and Support Rod ME-9355 Matches book Wood Splint 4 PASPORT Weather Anemometer PS-274 (or PASPORT Weather ) PASPORT Extension Cable PS-2500 Cardboard box (or other covering) 3 Exploring Microclimates Through Temperature Use a temperature sensor and current local weather conditions to measure relevant weather conditions at different locations. Determine the impact that a location's environmental conditions have on the microclimate of a given area. PASPORT Temperature * PS-225* 4 Exploring Microclimates with a GPS PASPORT Weather Anemometer PS-274 Use a weather anemometer sensor, a GPS position PASPORT GPS Position PS-275 sensor, and current local PASPORT Extension Cable PS-2500 weather conditions to MyWorld GIS SE-7382C measure relevant weather conditions (such as temperature, barometric pressure, relative humidity and dew point) at different locations. Students also collect GPS data and transfer the collected data to a computer to visualize how the weather conditions varied on a satellite image. USB flash drive or SE-7352C or SE-735C Or SE-7353C 4 PS-2870
Act Title Materials and Equipment Part 5 Metabolism of Yeast Use a carbon dioxide gas sensor to measure the production of carbon dioxide gas by yeast in aerobic and anaerobic conditions. Determine if high temperatures effect the respiration of yeast. 6 Rate of Photosynthesis of an Aquatic Plant Use a dissolved oxygen sensor to understand the amount of oxygen produced through photosynthesis in an aquatic plant in ambient light, bright light and darkness. 7 Soil ph Use a ph sensor to understand what kinds of soil in the local community would support agricultural crops, based on ph level. 8 Transpiration Use a barometer/low pressure sensor to explore the effects of environmental factors such as air movement on the rate of transpiration. Teacher Information PASPORT Carbon Dioxide Gas PS-20 PASPORT Extension Cable PS-2500 Beaker, 250-mL 2 Graduated cylinder 0-mL Graduated cylinder, 00-mL Grape juice 50 ml Hot Plate SE-8830 Mineral oil 5 ml Sampling bottle (included with Stirring rod L Yeast, dry package PASPORT Dissolved Oxygen PS-208* * PASPORT Fast Response PS-235* Temperature * Cloth, heavy, about 50-cm by 50-cm Elodea 5 or 6 Lamp, 00 W (or equivalent) Magnetic Stirrer and Magnetic Stir SE-7700 Bar Photosynthesis Tank or similar PS-252A setup Rubber stopper, #3 2 L PASPORT ph * PS-202* PASPORT Extension Cable PS-2500 Beaker, 250-mL 3 Digging device 400 ml Graduated cylinder, 00-mL Marking pen Measuring spoons set Paper towels 3 or 4 Sealable plastic bag 3 Soil sample, 60 ml 3 Stirring rod Wash bottle PASPORT Barometer/Low Pressure PS-23A PASPORT Extension Cable PS-2500 Bowl Fan Glycerin ml Knife Large Base and Support Rod ME-9355 Petroleum jelly 2 to 3 g Pipet 5
Master Materials and Equipment List Act Title Materials and Equipment Part 9 and ph Use ph and conductivity sensors to analyze the differences in how water ph changes when acid rain is added. Plant seedling, 2 to 5 cm tall Three-Finger Clamp SE-9445 Utility Clamp SE-9446 PASPORT ph * PS-202* PASPORT Conductivity * PS-26A* Beaker, 250-mL 4 Graduated cylinder, 00-mL Labels 6 Marking pen Pipet L 250 ml Small container (for diluted vinegar solution) Stirring rod sample, 250 ml 3 White vinegar 250 ml 20 Purification Use a ph sensor to PASPORT ph * PS-202* understand the effectiveness PASPORT Conductivity * PS-26A* of various treatments for Beaker, 250-mL 2 improving the quality of Coffee filter 2 water. 500 ml Egg whites 5 ml Erlenmeyer flask, 250-mL Funnel Polluted water L Stirring rod 2 Weather in a Terrarium Use a weather anemometer sensor in the microclimate PASPORT Weather Anemometer PS-274 of a terrarium to understand PASPORT Extension Cable PS-2500 changes in temperature, Small box or other support absolute and relative humidity, dew point, and barometric pressure. Terrarium (or suitable alternative) 22 EKG and Factors that Affect the Heart Use an EKG sensor to measure and observe the electrical activity of the heart muscle. PASPORT EKG PS-2 Electrode patches (included with 3 23 Exercise and Heart Rate Use a heart rate sensor to monitor the effect of physical exertion in relation to level of fitness. Determine the average heart rate before, during, and after exercise. PASPORT Hand Grip Heart Rate PS-286 24 Exercise and Respiration Rate Use a breath rate sensor to PASPORT Breath Rate PS-287 6 PS-2870
Act Title Materials and Equipment Part Teacher Information measure the resting respiration rates of individuals and determine whether exercise causes a change in respiration rate. 25 Muscle Fatigue Use a force sensor to PASPORT Force PS-204 determine grip strength and Rubber ball, tennis ball, or compare muscle fatigue in equivalent (approximately 7 cm hand muscles caused by diameter) isotonic ( same tension ) Timer (stopwatch or equivalent) and isometric ( same length ) exercise. 26 Regulation of Body Heat* Data Collection System Use a temperature sensor to PASPORT Fast Response PS-235 2 understand the extent that external conditions, such as ice water, moving air, or wearing gloves, cause changes in skin temperature. Temperature Probe Fan Glove or mitten Ice, crushed or cube Large bowl (or similar container) L * This activity requires 2 fast response temperature probes to be connected simultaneously. Please see the Lab Preparation section for details. 27 Volume of Breath Use a spirometer to explore the pulmonary function test (PFT) and the volume of breath. Tape or adhesive covers Paper Towel 5 to 6 L 2 pieces PASPORT Spirometer PS-252 Spirometer Mouthpiece PS-2522 per student *PASCO recommends the purchase of the Quality (PS-269) as an alternative to purchasing these items separately. The Quality comes with ph, dissolved oxygen, temperature and conductivity probes, all in one convenient package. 7
Master Materials and Equipment List Calibration materials If you want to calibrate various sensors, you will need the following: ph Item Quantity Where Used Buffer solution, ph (4) Buffer solution, ph (0) Beaker, small Wash bottle with deionized or distilled water 25 ml 25 ml 3 4,3,8,9,7,9,20 Dissolved Oxygen Item Quantity Where Used Clean electrode storage bottle Oxygen Gas 5 ml 0,6 Item Quantity Where Used Sampling Bottle (included with the 3 Carbon Dioxide Gas Item Quantity Where Used Sampling Bottle (included with the 7,6,5 8 PS-2870
Teacher Information Equipment List by Item This list shows each item needed for the activities and where the item is used. Items Available from PASCO Where Used PASPORT Carbon Dioxide Gas (with 7,6,5 Sampling Bottle) PASPORT Barometer/Low Pressure 5,8 PASPORT Oxygen Gas 2 PASPORT Fast Response Temperature Probe,0,6 PASPORT Hand Grip Heart Rate 23 PASPORT Extension Cable 5,7,6,2,4,8,,5,2,7 PASPORT ph 4,3,8,9,7,9,20 PASPORT GPS Position 4 PASPORT Temperature 3,,26 PASPORT Dissolved Oxygen 0,6 PASPORT Conductivity 9,20 PASPORT Force 25 PASPORT Breath Rate 24 PASPORT EKG 22 PASPORT Weather Anemometer 2,4,2 PASPORT Spirometer 27 Spirometer Mouthpiece per 27 student Photosynthesis Tank 6 MyWorld GIS 4 Quick Release Connector 5 Large Base and Support Rod 3,8,8, Magnetic Stirrer with Magnetic Stir Bar 3,8,6 Electronic Balance 5,9,,0 Hot Plate 5,0 Three-Finger Clamp 5,8 Utility Clamp 3,8,8 9