HARLECO Dyes & Stains Microscopy products to support your success

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HARLECO Dyes & Stains Microscopy products to support your success The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. A

For over 90 years, HARLECO brand dyes and stains have offered our customers outstanding contrast, color, and clarity in their sample specimens. HARLECO stain solutions offer not only the classical formulations, but also many new and improved modifications developed from years of experience. We use Certified Biological Stains and cgmp manufacturing for all HARLECO products to provide you with the highest quality stain solutions. In addition, we offer the Midas III-Plus Automated Stainer and a complete line of products for sample preparation. B

Hematology Reagents to identify diseases of the blood and blood forming organs The HARLECO product line offers ready to use staining solutions for blood and bone marrow samples that yield intense and reproducible results. Reproducibility can be enhanced by the use of phosphate buffer solution, which stabilizes diluted staining solutions. We offer buffers with different ph levels, so that you may obtain optimal staining results. For higher slide staining throughput or for preparing urgent lab samples, Hemacolor, the fast staining kit, can give very clean results in less than one minute. HARLECO Giemsa Stain Solution, Azure B 619-71 HARLECO Giemsa Stain Solution, Modified Azure Blend HARLECO Giemsa Stain Solution, Original Azure Blend 620-75 620G-75 Wright staining-blood smear Giemsa Stain Solution R03055-74 R03055-76 R03055-82 HARLECO Jenner Stain Solution 642-75 HARLECO May-Grunwald Solution HARLECO Wright Stain Solution HARLECO Wright Giemsa Solution, APHA HARLECO Wright Giemsa Solution, Fucillo 10 L 10 L 660-75 660-85 740-75 740-85 740-86 742-75 742-86 64571-95 64571-75 64571-85 HARLECO Wright Giemsa Stain Pack 65042-93 HARLECO Wright Stain Pack 65043-93 Leishman Stain Solution R03087-76 HARLECO Hemacolor Stain Set 1 PAC 65044-93 HARLECO Hemacolor Solution I 65044A-85 HARLECO Hemacolor Solution II 65044B-85 HARLECO Hemacolor Solution III 5044C-85 HARLECO Buffer, Phosphate ph 6.4 HARLECO Buffer, Phosphate ph 6.8 HARLECO Buffer, Phosphate ph 7.0 1217-75 1217-85 1218-75 1218-85 1219-75 1219-85 1

Cytology Reagents for brilliant and highly reproducible sensitive results To stain gynecological and clinical specimens, use standard Papanicolaou sample staining with hematoxylin Gill s solution. In addition, you can rely on our high quality alcohol and the xylene substitute, Neo-Clear, to further improve your results. HARLECO Hematoxylin, Gill I 65065-85 HARLECO Hematoxylin, Gill II 65066-85 HARLECO Hematoxylin, Gill III 65067-85 Papanicolaou stain gynecological sample HARLECO Hematoxylin, Harris Alum, Hg Free HARLECO EA Solution, Gill (with Eosin and Light Green) 638A-71 638A-85 65068-85 HARLECO Orange G Solution, Gill 65069-85 HARLECO Papanicolaou, OG-6 HARLECO Papanicolaou, EA-65 HARLECO Papanicolaou, EA-36 HARLECO Papanicolaou, EA-50 10 L 10 L 7052X-75 7052X-85 7052X-86 7054X-85 7054X-86 7058X-75 7058X-85 7062X-75 7062X-85 HARLECO Blueing Reagent 65354-85 HARLECO Acid Alcohol, 70% (3% HCl) 752X-75 HARLECO Acid Alcohol, 95% (5% HCl) 753X-75 HARLECO Alcohol, 100% 65347-85 HARLECO Alcohol, 95% 65348-85 HARLECO Alcohol, 80% 65349-85 HARLECO Alcohol, 70% 65350-85 Neo-Clear Xylene Substitute 65351-85 2

Histology Reagents for brilliant and stable results from the first to the last step in the process Precise specimen preparation is a necessity to get good slides for diagnostic work. We offer all of the products needed for preparing histology samples, including top quality fixing media and high purity solvents. The HARLECO stain results are color stable, reproducible and offer sufficient sensitivity to reveal all structural details. HARLECO Eosin Y Solution, Alcoholic Saturated 586X-7 HARLECO Eosin Y Solution, Aqueous, 1% PDC 588X-75 HARLECO Eosin Y Solution, Aqueous, 1% PDC 592-75 HARLECO Alcohol, 80% 65349-85 HARLECO Alcohol, 70% 65350-85 Neo-Clear Xylene Substitute 65351-85 Eosin Stain Solution, 5% R03040-74 HARLECO Schiff Reagent 2818-71 HARLECO Schiff Reagent, Hotchkiss/McManus 6073-71 HARLECO Gomori Trichrome 623-71 HARLECO Krystalon Mounting Medium 300 ml 64969-95 64969-71 Formalin 10% W/V, Neutral Buffered HARLECO Formalin, Neutral Buffered 10% 200 L R04586-76 R04586-82 R045-86 R04586-90 65346-85 65346-88 Formalin 10%, Neutral Buffered R04586B-82 Formalin 20%, Phosphate Buffered 1 CV R4587-01 R4587-86 H + E Stain - Liver tissue stained with eosin Y solution Formalin 10%, Acetate Buffered R04588-86 Formalin Neutral 10%, Carbonate Buffered R03379-82 R03379-86 HARLECO Formalin, Carsons 65115-88 SAF Fixative (Acetate Buffered Formalin) 200 L R04650-90 SAF Fixative with Triton (Acetate Buffered Formalin) 200 L R04561-90 Formaldehyde Kit Concentrates to make NBF R04669-01 HARLECO Bouin Fixation Fluid 7831-85 7831-88 HARLECO Fixative, Haupts 785-71 Albumin-Glycerol Fixative 100 ml R03203-70 R03203-74 HARLECO Blueing Reagent 65354-85 Mollifex Tissue Softener 65039-71 HARLECO Acid Alcohol, 70% (3% HCl) 752X-75 HARLECO Acid Alcohol, 95% (5% HCl) 753X-75 HARLECO Alcohol, 100% 65347-85 HARLECO Alcohol, 95% 65348-85 3

Microbiology Reagents for standard stains and reliable results with manual or automated staining HARLECO bacteriological standard stains for Gram and TB detection offer easy application and reproducible, stable results. The staining solutions can be used for manual as well as for automated staining procedures in the Midas III-Plus Automated Stainer. HARLECO Gram Staining Set, 4 x 1 PAC 65092-93 HARLECO Crystal Violet, Gram Solution HARLECO Safranin Solution, Gram HARLECO Iodine Solution, Gram (Stabilized) HARLECO Decolorizer, Gram Solution 1 PAC 1 PAC 1 PAC 1 PAC 65092A-95 65092A-85 65092B-95 65092B-85 65092D-95 65092D-85 65092E-95 65092E-85 HARLECO Iodine Lugol's 624-71 Safranin Solution, 1% R03131-74 HARLECO Fuchsin, Basic Solution 6039X-75 HARLECO Fuchsin, Carbol Solution, Ziehl-Neelson 6051X-75 HARLECO Fuchsin, Carbol Solution, Kinyoun 6033X-75 Methylene Blue Stain Solution, Loeffler s R03101-74 HARLECO Methylene Blue, Loeffler's 687X-75 Nigrosine Solution, Aqueous 100 ml R03418-70 Lactophenol Solution R03266-74 Lactophenol, Cotton Blue 100 ml R03465-70 Gram-color staining Gram-positive coccus 4

Midas III-Plus Automated Stainer The Midas III-Plus Automated Stainer is designed to work with HARLECO stains and is flexible enough to adapt to your current staining protocols. With a small footprint to allow use on the smallest benchtops, the Midas III-Plus Automated Stainer yields staining results that are more reproducible than manual staining. The six station unit holds either 20 or 30 slides. Up to 10 programs may be introduced, with each program comprised of as many as 29 steps. Save up to 50% on stain consumption as compared versus flood or spray techniques. Product Size Catalog No. Midas III-Plus Automated Stainer 64000H-94 20 position slide carrier each 64052-94 30 position slide carrier each 64052E-94 Water Bath each 64000WB-94 Water Bath Filters Package of two 64000WBF-95 Vessels & Lids White Package of Eight 64054W-95 Operators Manual 64000OP-94 Service Manual 64000SM-94 Multi-terge detergent 4L 65058-85 HARLECO Water, distilled ASTM type II 4L 6442-88 6442-88 Midas III-Plus Automated Stainer 5

Cell Marque Tissue Diagnostics Toll-Free (800) 665 7284 Phone (916) 746 8900 Fax (916) 746 8989 Email service@cellmarque.com www.cellmarque.com MilliporeSigma 290, Concord Road Billerica, MA 01821 Phone 1 (800) 645 5476 Email nacustomerservice@merckgroup.com www.emdmillipore.com 2017 Sigma-Aldrich Co. LLC. All rights reserved. Cell Marque and Sigma-Aldrich are trademarks of Sigma-Aldrich Co. LLC. Harleco, Midas, and Neo-Clear are trademarks of Merck KGaA, Darmstadt, Germany. Mollifex and Multi-terge are trademarks of American Type Culture Collection. Sigma-Aldrich Co., LLC is a subsidiary of Merck KGaA, Darmstadt, Germany. Lit. No. 03918 CM-17-04-0096 5/2017 Copyright 2017 EMD Millipore Corporation. All Rights Reserved. 6