Nematol t~7edit (2001) 29 207213 Institute of Biology, Faculty of Science, Universi& of Novi Sad, 21000 Novi Sad, Yz/goslavia Istituto di fvematologia Agraria, CNR 70126 Bari, Italy LONGIDORUS PIUS SP N (NEMATODA: LONGIDORIDAE) FROM MACEDONIA Summary Longido~*z~s pfus sp n,, a monosexual species found in the rhizosphere of Ca~pinus orientalis at Sveti Jovari Bigorski Monastery on the slope of Mount Bistra in Macedonia is described The species is characterized by its medium size (L=jO68 mm) and thick body (a=%68); anteriad abmptly tapering and laterally rounded lip region, continuous with the rest of the body; pocketsliaped amphids which are not bilobed at the base; long odontostyle (130147 pm); and short, rounded tail L pius sp n has four juvenile developmental stages; the first stage with digitate tail L pzus sp n reseriibles L picenus and L neuesi A Longido~pus species, unknown to the best of our knowledge, was extracted from soil samples collected in Apri1 2000 from the rhizosphere of Carpinus olpientalis at Sveti Jovan Bigorski Monastery on the slope of Mount Bistra (Macedonia) The population is described here as L pius sp n Materia1 and methods Nematodes were extracted by Cobb's wet sieving technique Specimens were killed by hot FP 41 and processed and mounted on perinanent slides in dehydrated glycerin Measurements were made with an eyepiece scale, except body length, which was determined with the aid of a drawing tube and map measurer Description LONGIDORUS PIUS sp n (Table I; Figs 14) Female habitus more or less ventrally curved with the curvature inore pronounced posteriad in some specimens Body of medium size, robust and cylindrical, tapering 17e1y gradually towards anterior Cuticle 56 pin thick at midbody, smooth in appearance, sometimes with very fine transverse striations which are always visible in the tail region Lip region continuous with the rest of the body, tapering abruptly anteriad and laterally rounded Labial papillae prominent Ainphidial pouches large, pocketsliaped without distinct basal lobes Odontostyle, odontophore and guiding ring typical of the genus A mucro is visible at the base of the odontophore or just behind it Oesophagus dorylaimoid, with the basal bulb occupying 1/4 1/5 of the total oesophagus length and measuring 108141 pm long and 3336 pm wide; it contains three glandular nuclei Oesophagointestinal valve cylindrical Vulva a tranverse slit, at inidbody; vagina extending to 4060% of corresponding body diameter; thick walled pars proximalis vaginae 2131 pm long Gonads amphidelphic, of approximately the same length and structure; ovaries reflexed Prerectum 222 510 pm long (n= 101, rectum as long as 0609 body diameter at anus Tail bluntly conoid,
TABLE I Morphonzetrics oj'longidonis pius sp 72 j'rorn Macedo7zia Holotype Pardtypes n Q 25 Q9 20 51 21 J2 13 J3 9 54 L (mm) 532 589k047 1 40+016 217k027 319k028 4351032 (50682) (12169) (l 58267) (253362) (38350) a 595 639k234 4541214 4631244 508k20 569k364 (584684) (40495) (416507) (472547) (509618) b 101 1121099 441042 581072 73k06 85k061 (84128) (3954) (4774) (6486) (7994) C 1189 1353+1010 3011236 577k718 809k64 974k1105 (11461526) (263349) (49718) (699926) (8041168) C' 066 065k004 193k017 0991011 076+006 072k005 v (057074) (16723) (079118) (068086) (064079) 499 506k127 (480545) Odontostyle pm 1346 13651445 719k168 827k187 1042k354 1232a372 (1301473) (675737) (794869) (9621087) (11751295) Odontophore pm 80 806k384 4751198 576k445 6711377 749k221 (738875) (42550) (488638) (575713) (725788) Total stylet prn 2146 2171+602 1193k283 14031443 1713k497 1981k573 (20632298) (11371237) (1301456) (16371775) (1902083) Replacernent 839k228 1016k529 1175k166 1331k330 odontostyle pm (8089) (8941088) (1151214) (12741372) Ora1 aperture 375 387k194 2071065 251k113 303k131 3521113 to guiding ring pm (350419) (194219) (231275) (275331) (331363) Tail pm 447 4431297 465k385 3761275 3951338 441274 (393503) (403525) (321418) (343453) (40489) J (hyaline portion 169 1751087 17k117 107_+121 ii9+116 1441148 of tail) pm (159197) (15197) (84125) (10138) (113163) Body diam at lip 225 217k072 98k018 126+042 159k092 187k053 region pm (208234) (9410) (117138) (138175) (1781941 Body diam at 425 434k214 173k045 2241116 3041130 36a420 guiding ring pm (397472) (166184) (204247) (281322) (25640) Body diam at base 763 780k376 303k258 427k475 555k399 6751269 of oesophagus pm (716854) (272338) (338513) (463625) (642725) Body diam at mid 894 9361680 309k331 4711745 63k627 764k496 body or vulva pm (80105) (272375) (33860) (463729) (713853) Body diain 675 6871376 243rt289 384k414 519k428 614+354 at anus pm (61375) (213294) (30466) (438604) (55653) Body diam at 475 471k254 122k083 241328 34k313 405k261 beginning of J pm (413513) (109138) (188313) (288388) (35438)
Fig 1 Longicio~7~spius sp n,: A, female anterior region; BC female tail; DG, tail of J1, J2, J3 and 14 stage, respectively rounded to almost hemispherical, bearing two Type baliitat arzd locality Rhizosphere of or three caudal pores on each side Cnrpi~zus orieiztalis Mill at Sveti Jovan Bigorski Male not found Monastery (UTM: DM6O) on the slope of Mount Juveniles separate into four groups (Fig 4) Bistra, Macedonia The first juveniles stage with digitate tail Type material Holotype, 10 female and 30
Fig 2 Phommicrographs of L pnrs sp n: A, femaie amior regiwi; RD, fede amphidid p&; E, ju of the: don e with he oclontophorie, F, dva region; G, ammior uww; HI, fede tail 210
Fig 3 Photomicrographs of juvede stages of L piw sp n AD, &or mil ofji, Jz, J3 and J4 1~ regian of JI, J2, J3 d J4 smge, respectivel~ EH,
C, juvenile paratypes in the collection of the Istituto di Nematologia Agraria del Consiglio Nazionale delle Ricerche, Bari, Italy; 11 female and 33 juvenile paratypes, Institute of Biology, Novi Sad, Serbia; two female paratypes, CABI Bioscience Centre, Egham, United Kingdom; two female paratypes, Plant Nematology Laboratory Collection, United States Department of Agriculture, Beltsville, Maryland, USA Diagnosis and relationships Longidorus pius sp n is a monosexual species of medium size (~=5068 mm) and thick body (a = 5868), with laterally rounded lip region, continuous with the rest of the body, pocketshaped amphids which are not bilobed at the base, long odontostyle (130147 pm), and short, rounded tail; juvenile developmental stages separate into four groups; digitate tail in the first stage juvenile The code for identifying the new species when using the polytomous key of Chen et al (1997) and ~oof and Chen (1999) is: A 56, B 45, C34,Dl,El,F23,Gl,Hl,Il Longidoms pius sp n is similar to L pice~zus Roca, Lamberti et Agostinelli, 1985 and L nevesi Macara, 1986 However, it differs from both in the amphids not being bilobed at the base Further, L pius differs from L picenus in its lower value of a (8090 in L picenus) and C (155201 in L picenus) ratios and from L neuesi in its shorter body (8 mrn in L neuesi), lower a ratio value (72102 in L neuesz] and less rounded lip region (almost hemispberical and pointed in L neuesi) _ Odontostyle Replacement odontostyle n = 89 J2 o 6&OB0 % J3 (2 (:ti) 1 (2 L* ai e* J1 8 0 J ( 1 8 3 &A*& Females J4 [bo O (3 pe 't 0 I I I l l I l 1 I I l Body length (mm) Fig 4 Scatter diagram separatiiig juveniles and females of L pius sp n
Acknowledeement a We thank Ivan Dulic' laimoidea) Fz~ndamental and Applied Nematology, 20: 1528 'C', Naftagas> Novi giving Loor p, A, A and CnrN Q,, 1999, A revised polytomous t0 an Olyinpus BX50 photomicroscope kev for the identification of snecies of the eenus Lonaido'rus Micoletzky, 1922 (~ematoda: ~or&imoidei] Supplement 1 Nematology, 1: 5559 MACARA M, 1986 TWO new species of Longidorus (Ne Literature cited matoda: Longidoridae) associateci with forest plants in Portugal Nematologica, 32 (1985): 410423 CHEN Q, HOOPER D J, LOOF P A A and Xu J, 1997 A re ROCA F, LAMBERTI F and AGOSTINELLI A,, 1985 Three new vised polytomous key for identification of the genus species of Longidonrs (Nematoda, Dorylaimida) from Longidorus Micoletzky, 1922 (Nematoda: Dory Italy Nematologia Meditewanea, 12 (1984): 187200 Accepted for publication on 18 June 2001