PEI Mussel Monitoring Program Report. Technical Report # 252

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PEI Mussel Monitoring Program 2013 Report Technical Report # 252 By Gary Smith Mussel Monitoring Technician & Aaron Ramsay Aquaculture Biologist Prince Edward Island Department of Fisheries, Aquaculture and Rural Development Aquaculture Division Montague, PEI 548 Main Street C0A 1R0

ACKNOWLEDGEMENTS The PEI Department of Fisheries, Aquaculture and Rural Development wishes to acknowledge the cooperation of the mussel growers whose leases were used as sites for the collection of mussel and water samples for the Mussel Monitoring Program, as well as others who assisted in the delivery of this program. As well, the Department would like to acknowledge Tommy Joe MacDonald Jr. and Tyler Bernard for their work in sample collection and analysis. - i -

TABLE OF CONTENTS ACKNOWLEDGEMENTS... i SUMMARY... 1 INTRODUCTION... 4 MATERIALS AND METHODS... 5 Equipment... 5 Methods and Procedures... 6 1. Spat-fall Prediction... 7 2. Mussel... 8 3. Water Temperature... 8 4. Phytoplankton Monitoring... 9 5. Tunicate Larvae... 10 6. Predator and Fouling Monitoring... 10 Reporting... 11 RESULTS AND DISCUSSION... 11 APPENDIX I Mussel Monitoring Data by Area... 16 APPENDIX II Maps of Sample Sites... 39 APPENDIX III Water Temperature Graphs for Sample Sites... 54 APPENDIX IV Mussel Steamed Information... 61 APPENDIX V Maps Displaying Current Known Range of Invasive Tunicates in PEI... 71 - ii -

SUMMARY The information collected from the Mussel Monitoring Program (MMP) is provided to mussel growers and processors to assist in the management of their mussel farms and mussel harvesting plans. Staff of the Department of Fisheries, Aquaculture and Rural Development (DFARD) collected information related to mussel spat-fall, mussel meat yield, water temperature, the presence of potentially toxic algal species, tunicate larvae, and the presence of predators and fouling on mussel seed and grows-out lines. The information was collected from 33 mussel spat collection and growing areas over a period of 31 weeks in 2013. Information on mussel spat-fall prediction was collected from April 30 th to July 2 nd and on potentially toxic phytoplankton from September 4 th to November 27 th. Monitoring for the presence of potentially toxic phytoplankton was conducted in cooperation with the Canadian Food Inspection Agency (CFIA). The cooperation between CFIA and DFARD has resulted in a more efficient sampling program. In addition, water samples from mussel grow-out areas were examined for the presence of mussel larvae from July to late November. Growers often delay raising their crop and the socking of new mussel seed in the fall until the mussel spawning period is over and larvae can no longer be detected. This information assists the growers in avoiding a second mussel set on both new and old mussel crop. Mussel landings in 2012 were 50.0 million lbs, an increase of 4.0 million lbs over the landings in 2011. Landings for 2013 were not finalized at the time of printing of this report. The meat yield values of mussels collected from the monitoring stations in 2013 varied greatly (17-50 Canadian meat yield values) from area to area without evidence of apparent trends. There was an excellent set of mussel seed in most areas this year. Treatment for starfish, green algae and tunicates is very important in maintaining a good set of spat on collectors. There were no reports of seed shortage this year. Temperature data collected at each of the sample sites were within normal ranges for mussel growth and performance. - 1 -

There were high counts of Pseudo-nitzschia sp., from several north shore bays, in the water samples collected for the toxic phytoplankton monitoring portion of the program, however no toxins were detected in the analysis of mussel tissue. This is the first time since the fall of 2006 that high concentrations of Pseudo-nitzschia sp have been detected in water samples. There have been no elevated levels of domoic acid detected in shellfish since the fall of 2006 and, as a result, there were no closures of mussel harvesting areas due to presence of toxins. Tunicates continue to cause fouling problems for many mussel growers, with high densities of vase tunicates occurring in Montague River, Brudenell River, St. Mary s Bay, Murray River, Boughton River and Cardigan River. The vase tunicate continues to be observed in Souris River. For the fifth consecutive year, a joint intensive survey was conducted in the Nine Mile Creek area by Fisheries and Oceans Canada (DFO), the Atlantic Veterinary College (AVC) and our Department in mid September. No vase or clubbed tunicates were observed during the survey; however, the density of the violet tunicate remains high in the area. The density of the clubbed tunicate remains high in the March Water portion of Malpeque Bay and in Darnley Basin. Two clubbed tunicates were found on oyster gear in Savage Harbour in the spring of 2012; however, an intensive follow-up survey by DFARD and DFO was conducted in 2012 (and again in 2013 by DFARD) and this species was not detected. DFARD and AVC completed a follow-up survey in Tracadie Bay in September in response to seed being transferred to the area from Grand River last year, prior to a large clubbed tunicate being detected on a seed collection line. DFARD also completed a dive survey in Rustico Bay in mid August. Colonial tunicates have become well established in the bay; however, no solitary tunicates were observed. The clubbed tunicate was detected for the first time in Egmont Bay on oyster gear in November 2013. Colonial tunicates have become more abundant in several other north shore bays, including Darnley Basin, Southwest River and New London Bay. The two colonial tunicate species remain at high levels in Tracadie Bay, St. Peter s Bay, Nine Mile Creek and Savage Harbour. For information on the locations of all four aquatic invasive tunicates in PEI, see the invasive species locator maps in Appendix V. Tunicate larvae numbers, listed by species, are shown in Appendix I. Vase tunicate larvae were present in water samples from June 5 th until November 14 th. Peak vase tunicate larval count was - 2 -

from water samples collected from Murray River on September 17 th, with 200 larvae being counted in a 150 L water sample. Clubbed tunicate larvae were present in water samples collected in March Water and Darnley Basin from mid June until mid October, peaking in Darnley Basin on August 7 th, with 159 larvae detected. Information collected over the 2013 season was communicated to mussel growers via the use of a voice mail system and also through the Mussel Monitoring website at www.gov.pe.ca/go/musselmonitoring. - 3 -

INTRODUCTION The Prince Edward Island Mussel Monitoring Program (MMP) is a technical service provided to cultured mussel growers and processors by the Department of Fisheries, Aquaculture and Rural Development (DFARD). The MMP has operated annually since 1982 during the ice-free season providing mussel growers and processors with a variety of information to assist them in the management of their operations. Information is collected for the MMP on mussel spat-fall prediction, mussel meat yield analysis, water temperature, the detection and estimation of the numbers of potentially toxic algae species (such as the toxin producing diatom, Pseudo-nitzschia sp., and dinoflagellates, Alexandrium sp. and Dinophysis sp.), the presence and number of tunicate larvae, and the presence and quantity of predators and fouling organisms. As well, information on mussel meat quality of harvestsized mussels collected at the sample sites (i.e. meat yield and spawning condition) is made available over the season to the mussel growers and processing plants through the MMP. This information assists the processing plants in ensuring the consumer receives a high quality product. The department has expanded the role of the MMP over time in response to requests for additional information by the mussel industry. As well, the information collected for this program is often utilized by other government and academic research agencies and additional information has been collected for researchers when possible. The MMP has provided this assistance without substantially re-directing its resources from its mandate as a technical advisory service to the Prince Edward Island (PEI) mussel growers. In 2012, the cultured mussel industry produced 50,023,365 lbs of product for market with a landed value of $30,014,000, which was an increase in both landings and value, as compared to 2011 (Figure 1). The mussel industry is an important contributor to the PEI economy and has resulted in the creation of an estimated 1,500 full-time jobs and numerous spin-off industries. The economic value of the industry to PEI is estimated at over $60 million dollars. - 4 -

Figure 1. PEI mussel landings and values from 1980 to 2012. The mussels grown in PEI waters are of high quality because the growing areas are abundant in food and have excellent water exchange. The rivers and bays on PEI are well sheltered from prevailing winds making them ideal for mussel aquaculture. The objective of this report is to document the information that was communicated to mussel growers during the 2013 season through either the MMP website or through the dedicated phone message system. MATERIALS AND METHODS Equipment 17' Boston Whaler, 90 hp Mercury outboard motor and Shorelander boat trailer YSI temperature meter, Vemco automated temperature recorders, water pump (50L/minute capacity), 64 micron nitex screen for collecting mussel larvae, 20' clear plastic tubing (1.5" in diameter) and 2.5 gallon container Electronic weigh scale, calipers and cooking equipment for meat yield analysis Microscope, slides and pipettes for mussel larvae and phytoplankton observation Vacuum pump and filtering apparatus for phytoplankton slide preparation - 5 -

Field books, sample bags and cooler Computer and printer to analyze and store data Methods and Procedures The data collected by the MMP is to provide information on six main areas related to mussel aquaculture: 1. Mussel spat-fall prediction 2. Mussel meat yield 3. Water temperature 4. Presence of potentially toxic phytoplankton 5. Tunicate larvae sampling 6. Predators and fouling organisms Thirty-three mussel growing/spat collection areas are sampled weekly for the MMP (Figure 2). Maps demonstrating the specific locations of each of the sample sites are displayed in Appendix II. Figure 2. Sample collection sites for the Mussel Monitoring Program. - 6 -

1. Spat-fall Prediction The numbers and size range of mussel larvae were determined from water samples collected weekly at each of the sample locations from late April until early July. This information was used to assist mussel growers in predicting the timing and abundance of mussel spat-fall. The growers use this information to determine when to either deploy, or to raise and clean their collectors in order to maximize spat collection. Mussel larvae information was also collected from July to late November at some grow-out sites to provide growers with information on mussel second set. Some growers attempt to avoid or reduce the amount of second set from settling on market mussels by keeping the mussels submerged until mussel larvae are no longer detected in the water column. The growers also avoid the second set from settling on newly socked mussels by delaying the socking process until after the second set is complete. To collect mussel larvae for this analysis, water samples are pumped from the top two to three meters of the water column through a 64 µm screen at the rate of 50 liters per minute for three minutes (Figure 3). The mussel larvae become trapped on the screen surface and are washed off with 10 ml of previously screened seawater into a sample bottle. A 1 ml subsample of the larval concentrate is examined utilizing a compound microscope to determine the abundance and average size of the mussel larvae. The quantity of mussel larvae present is quantified according to the following scale: Low = 1 5 Medium = 6 15 High = >15 Figure 3. MMP field crew collecting water samples and water quality information. - 7 -

2. Mussel Market size mussels were held in wire cages at each monitoring station for meat yield analysis. Samples for meat yield analysis were collected from each of the monitoring stations from April 27 th to November 27 th. The following procedure is used for the determination of mussel meat yields: A. Thirty market mussels, 55 mm or greater in length, (generally 18-24 months old) are randomly selected and cleaned in fresh running water. B. Mussels are steamed in the absence of water for ten minutes, and then the meats are shucked from the shells. C. The steamed meat yield, expressed as a percentage, is determined according to the formula: = Steamed Meat Weight x 100 (Steamed Shell + Steamed Meat Weight) D. The European meat yield, expressed as a percentage, is determined according to the formula: European = Steamed Meat Weight x 100 Raw Weight of Sample Mussel meat yield information is beneficial to growers to assist in the determination of the mussel spawning condition in a river system. The information, when collected over a long-term basis, is of interest in the analysis of potential trends in mussel condition. This information is displayed graphically in Appendix IV. 3. Water Temperature Water temperatures were recorded from 1 to 2 metres below the surface at each site, at the time of each site visit, throughout the season (Figure 3). In addition, automatic temperature recording devices, set to record hourly temperature readings, were placed approximately 2 metres below the surface in several of the mussel growing areas. The hourly collected temperature data provided additional information on water temperature profiles throughout the ice-free season. This information assists growers in comparing temperature conditions with the development of mussel spawning condition over the season in various river systems. As well, in the event that shellfish mortality occurs, it is beneficial to have the information on temperatures over the season to assist in the investigation of the cause of the mortality. Temperature profiles taken - 8 -

from each of the mussel monitoring stations can be found in Appendix III and also in Appendix I for specific sampling days. 4. Phytoplankton Monitoring The monitoring for the presence of potentially toxic phytoplankton began on September 4 th and continued until November 27 th in 2013. It is during this time period that toxic algae blooms have most frequently occurred in PEI waters. This portion of the program was conducted in conjunction with the Canadian food Inspection Agency (CFIA) who share in the collection of samples with staff of the MMP. The procedure utilized to collect, identify and quantify potentially toxic algae is documented in the DFO procedures manual A field and laboratory manual for the collection, identification and enumeration of toxic marine phytoplankton by John C. Smith and Kevin Pauley. Department staff collected water samples weekly from sites in Boughton River, Brudenell River, Cardigan River, Covehead Bay, Murray River, Savage Harbour, St. Mary s Bay, St. Peter s Bay and Tracadie Bay. Mussel samples were also collected by department staff from these sites for the CFIA toxin monitoring program. CFIA staff collected water and mussel samples from Darnley Basin, Lennox Channel, March Water, New London Bay, and Rustico Bay for both programs. Information on the identification and quantity of potentially toxic phytoplankton species observed in the water samples were reported to CFIA and also were made available to growers on the MMP website and voice mail message system. The numbers of algal cells observed were reported according to the following scale: Trace = 1-1000 cells/l Low = 1000-50,000 cells/l Medium = 50,000-350,000 cells/l High = greater than 350,000 cells /L The results of the phytoplankton analysis for 2013 are reported in the results section of this report (Appendix I). The information collected from this portion of the program is beneficial to provide both industry and CFIA with an early warning of the possibility of a bloom of potentially toxic algae occurring in an area. The presence of toxins in mussel tissue is monitored by CFIA, - 9 -

and closures are based upon these levels; however, the information on the types and quantity of phytoplankton present in the water column is invaluable as early warning that an event might occur. If the samples show that numbers of a potentially toxic algae species are present and increasing in a river system CFIA may increase their sampling of mussels from the area and industry can plan future mussel harvests avoiding the algae bloom and ensuring that a safe product is sold to the market place. 5. Tunicate Larvae The numbers of tunicate larvae (vase, clubbed, violet, golden star) were counted in water samples collected from tunicate infested areas (see Appendix I for numbers of tunicate larvae). The purpose of obtaining the larvae numbers was to: inform growers when the tunicates start and stop spawning, the species of tunicate larvae present, and to provide an indication of the amount of larvae that were present over the season. Recent research results have shown that there may not be a direct correlation between the timing and density of tunicate recruitment and the observation of tunicate larvae and larvae densities in the water samples collected for MMP. However, this information is still considered to be worthwhile to collect and may show an indication in trends in changes in tunicate populations over time. Tunicate counts were conducted weekly from mid-june to early December. Water samples were collected by pumping water through a 64 µm sieve using a water pump at the rate of 50 L per minute for 3 minutes (a total of 150 L per sample). Tunicate larvae were identified and counted in the entire water sample. The larvae counts were made available for the growers on the MMP website and voice mail message system (1-800-454-3233). 6. Predator and Fouling Monitoring Spat lines were examined for the general appearance of the crop and for the presence of predators (such as starfish and sea ducks), fouling organisms such as algae, hydroids, tunicates or sea anemones and also for the presence of silt. Mussel growers are notified if large numbers of starfish were observed on spat lines, or of any other potential problems that are noted by technical staff. In the fall of 2013, staff worked with Bob Thompson, a consultant hired by the PEI Aquaculture Alliance to coordinate sea duck surveys and to test new techniques to deter ducks from predating on cultured mussels. If growers observe any unusual fouling organisms or - 10 -

aquatic invasive species that are outside of their known range they are asked to either contact this department or Fisheries and Oceans Canada (DFO). Staff of either department will be available to identify the organisms and provide more information on the species. Information and maps showing the current known ranges of all aquatic invasive tunicates in PEI waters is available on the MMP website, in Appendix V of this report, or may be obtained by contacting staff of either our department or DFO. Reporting The information collected from the MMP is made available throughout the field season through a telephone voice mail message system and also through the mussel monitoring website. In addition, the previous year s MMP reports are available on the DFARD website. Mussel growers are able to access this information at any time. The telephone numbers are Charlottetown exchange 368-6510 and long distance 1-800-454-3233. The web address is www.gov.pe.ca/go/musselmonitoring RESULTS AND DISCUSSION Information collected on quantity and size ranges of mussel larvae, meat yields, water temperature, numbers of potentially toxic algae and tunicate larvae from each monitoring location are presented in the tables in Appendix I. A high quantity of mussel seed was collected in most areas in 2013. High numbers of setting size mussel larvae were observed in water samples at most locations from mid June until early July. In 2013, high numbers (greater than 350,000 cells/l) of Pseudo-nitzschia sp. cells (Figure 4) were observed in some locations. In past years, there were frequent fall blooms of Pseudonitzschia sp., which provided a significant food source for cultured mussels prior to the winter period. However, there have been no closures to mussel harvesting due to domoic acid toxicity since 2006. For example, in Cardigan River the numbers of Pseudo-nitzschia sp., (a non-toxin producing form), peaked in the late fall of 2001 at 5.6 million cells/l of seawater and in 2002 at - 11 -

9.3 million cells/l. In 2013, the peak numbers of Pseudo-nitzschia sp. cells was 3.2 million cells/l (Lennox Channel). Since 2007, there have been extensive blooms of Chaetoceros sp., along with other algae species, which are also excellent food sources for mussels. Figure 4. Pseudo-nitzschia sp., a potentially toxic phytoplankton species. Invasive tunicate species continue to cause fouling problems for the mussel industry. There are four invasive tunicate species located in PEI waters: the solitary clubbed and vase tunicates and the colonial violet and the golden star tunicates (see Appendix V for maps showing the most current tunicate distribution). The clubbed tunicate remains at high levels in the March Water area of Malpeque Bay and in Darnley Basin, while numbers continue to remain low in New London Bay, Orwell / Vernon Rivers, Souris River and Grand River. Two clubbed tunicates were found on oyster gear in Savage Harbour in the spring of 2012; however, an intensive follow-up survey by DFARD and DFO was conducted in 2012 (and again in 2013 by DFARD) and this species was not detected. DFARD and AVC completed a follow-up survey in Tracadie Bay in September in response to the transfer of mussel seed from Grand River last year, prior to a large clubbed tunicate being detected on a seed collection line. DFARD also completed a dive survey (Figure 5) in Rustico Bay in mid August. Colonial tunicates have become well established in these bays; however, no solitary tunicates were observed. The clubbed tunicate - 12 -

was detected for the first time in Egmont Bay on oyster gear in November 2013. The density of the vase tunicate continues to be very high in the Montague and Brudenell Rivers, St. Mary s Bay, Murray River, Cardigan River and Boughton River. A population remains in the Souris River, where it was first identified in 2010. Colonial tunicates have been detected in all mussel production areas on PEI, with the exception of North Lake. Figure 5. DFARD staff completing dive survey (Bideford River) for the presence of aquatic invasive species. Currently, there are restrictions on shellfish transfers into and between areas due to tunicate infestations. For the most recent information on the restrictions please contact the local DFO office. Maps depicting the locations of the aquatic invasive tunicates in PEI are displayed in Appendix V, and may also be viewed on both the MMP and DFO s websites. Tunicate larvae numbers, by species, which were detected in the water samples, are shown in Appendix I. Vase tunicate larvae were present in water samples from June 5 th until November 14 th. Peak larval count of the vase tunicate was in Murray River on September 17 th with 200 larvae being counted in a 150 L water sample. Clubbed tunicate larvae were present in water samples collected in March Water and Darnley Basin from mid June until mid October, peaking in Darnley Basin on August 7 th with 159 larvae detected. - 13 -

Sea duck predation on newly socked mussels continues to be a problem in PEI. With the apparent trend of warm weather occurring later into the fall and milder winters with ice cover occurring for shorter periods, there has been more opportunity for ducks to feed on newly socked mussels. Sea duck predation has been observed on mussels in the spring of the year since 2006. This appears to be a new trend in feeding behavior of migrating sea ducks, (previously predation problems were only noted in the fall of the year). The most problematic species are scaup, longtailed ducks and scoters (Figure 6). Figure 6. Problematic duck species, including scaup (Top), long-tails (Middle) and scoters (Bottom). - 14 -

DFARD, worked jointly on a project with DFO, the Canadian Wildlife Service (CWS), the Prince Edward Island Aquaculture Alliance (PEIAA) and Industry to investigate new deterrent techniques (i.e. Phoenix Wetlands Wailer, see Figure 7), and to conduct surveys to determine sea duck population dynamics. Figure 7. 'Phoenix Wetlands Wailer being tested as sea duck deterrents. Despite the challenges facing the mussel industry, including sea duck predation and tunicate fouling, landings continue to grow each year. In 2012, landings reached a new high of 50,023,365 lbs, with a landed value of $30,014,000. The industry, as a whole, has shown a remarkable ability to innovate, developing new technologies and growing practices to overcome these challenges. - 15 -

APPENDIX I Mussel Monitoring Data by Area - 16 -

Baltic River Date Water Temp Mussel larvae Size (microns) Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 28 11.0 0 0 0 0 - - - - - - - - - - - - - - - - June 06 16.7 Med 90-120 100 0 - - - - - - - - - - - - - - - - June 11 15.0 High 90-190 100 0 - - - - - - - - - - - - - - - - June 20 19.0 High 90-220 100 0 - - - - - - - - - - - - - - - - June 27 17.4 High 110-240 100 0 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. No setting larvae were observed during sampling. Bentick Cove Date Water Temp Mussel larvae Size (microns) Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 28 12.3 Low 90-110 100 0 - - - - - - - - - - - - - - - - June 04 15.6 Low 90-130 100 0 - - - - - - - - - - - - - - - - June 10 14.4 Med 90-200 100 0 - - - - - - - - - - - - - - - - June 17 16.6 High 90-260 95 5 - - - - - - - - - - - - - - - - June 27 18.9 High 110-380 70 30 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 17, 2013-17 -

Boughton River Date Water Temp Mussel larvae Size (microns) Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 08 9.6 0 0 0 0 50 32 - - - - 0 May 16 8.6 Med 90-110 100 0 45 29 - - - - 0 May 23 9.8 Low 90-110 100 0 46 31 - - - - 0 May 30 11.1 Med 110-140 100 0 37 22 - - - - 0 June 04 13.3 Med 90-160 100 0 50 31 - - - - 0 June 12 14.2 High 90-240 100 0 38 21 - - - - 0 June 19 16.7 High 90-270 90 10 36 18 - - - - 13C June 25 18.6 High 110-320 80 20 31 15 - - - - 6C July 03 19.2 High 110-310 70 30 31 15 - - - - 3C July 09 21.0 High 110-330 60 40 30 14 - - - - 5C July 16 24.1 High 110-360 50 50 32 16 - - - - 10C July 25 21.7 Med 120-380 30 70 32 16 - - - - 12C July 31 22.1 Low 110-380 50 50 30 15 - - - - 8C Aug 06 21.5 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 5C Aug 13 20.7 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 9C Aug 19 20.3 Med 120-360 20 80 - - - - - - - - - - - - 21C Aug 26 19.7 Low 120-220 100 0 - - - - - - - - - - - - 189C Sep 04 19.1 Low 260-390 100 0 27 12 0 121C Sep 10 15.8 Med 260-380 100 0 32 17 0 107C Sep 18 16.5 Low 180-260 50 50 29 14 0 95C Sep 25 17.2 Low 280-300 0 100 29 14 400 148C Oct 02 15.9 Low 320 0 100 27 13 0 59C Oct 09 13.6 0 0 0 0 27 13 0 9C Oct 16 12.8 0 0 0 0 27 13 0 7C Oct 23 12.3 0 0 0 0 27 13 0 12C Oct 30 9.1 0 0 0 0 26 13 0 2C Nov 06 8.7 0 0 0 0 27 15 700 1C Nov 14 6.4 0 0 0 0 28 16 900 0 Nov 27 3.0 0 0 0 0 29 17 0 0 The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 19, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet - 18 -

Date Water Temp Mussel larvae Size (microns) Brudenell River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) May 09 8.2 0 0 0 0 45 29 - - - - 0 May 16 5.7 Low 90-110 100 0 44 28 - - - - 0 May 23 7.6 Low 90-110 100 0 44 29 - - - - 0 May 29 8.5 0 0 0 0 - - - - - - - - - - - - 0 June 05 9.9 High 90-200 100 0 39 23 - - - - 1C June 13 12.4 High 90-220 100 0 40 26 - - - - 1C June 18 15.6 High 90-250 95 5 36 21 - - - - 1C June 26 16.0 High 90-360 80 20 37 21 - - - - 3C Tunicate Larvae July 03 17.5 High 110-360 60 40 35 19 - - - - 5C 2S July 10 20.5 High 110-360 50 50 35 19 - - - - 5C 6S July 18 21.1 High 110-360 60 40 35 20 - - - - 6C 5S July 24 20.0 Med 120-400 20 80 32 18 - - - - 5C 3S July 30 20.6 Low 110-360 50 50 31 16 - - - - 4C 2S Aug 05 20.5 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 4C 2S Aug 13 19.0 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3C 5S Aug 20 19.4 Med 120-380 20 80 - - - - - - - - - - - - 62C 4S Aug 27 19.2 Low 130-300 50 50 - - - - - - - - - - - - 18C 3S Sep 04 18.9 Low 190-380 50 50 28 16 0 15C 3S Sep 10 12.8 Low 280-320 0 100 31 18 0 8C 1S Sep 18 15.8 Med 170-360 20 80 34 20 0 7C 2S Sep 25 16.0 Low 280-340 0 100 35 22 0 12C 2S Oct 02 15.8 Low 280-320 0 100 34 19 0 5C Oct 10 13.8 0 0 0 0 33 18 0 2C Oct 16 13.1 0 0 0 0 35 19 400 1C 1S Oct 23 12.3 Low 320 0 100 36 20 0 8C Oct 31 9.1 0 0 0 0 37 22 0 1C Nov 06 9.0 0 0 0 0 37 24 0 0 Nov 14 6.4 0 0 0 0 39 24 700 1C The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 18, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet - 19 -

Date Water Temp Mussel larvae Size (microns) Cardigan River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) May 08 6.9 0 0 0 0 - - - - - - - - - - - - 0 May 16 5.7 0 0 0 0 - - - - - - - - - - - - 0 May 23 7.1 0 0 0 0 - - - - - - - - - - - - 0 May 29 8.3 0 0 0 0 - - - - - - - - - - - - 0 June 04 12.7 Low 90-130 100 0 - - - - - - - - - - - - 0 June 12 11.7 High 90-200 100 0 - - - - - - - - - - - - 0 June 19 14.6 High 90-250 98 2 - - - - - - - - - - - - 3C June 25 15.8 High 110-330 85 15 - - - - - - - - - - - - 3C July 03 17.9 High 110-380 70 30 - - - - - - - - - - - - 3C July 09 19.4 High 90-340 60 40 35 16 - - - - 3C July 16 21.4 High 110-380 50 50 33 15 - - - - 6C July 25 20.7 Med 110-360 50 50 31 14 - - - - 6C July 30 20.8 Med 120-390 20 80 31 14 - - - - 6C Tunicate Larvae Aug 06 19.2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1C 2S Aug 13 19.5 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0 Aug 19 19.4 Low 110-320 20 80 - - - - - - - - - - - - 1C Aug 26 19.0 Med 120-380 30 70 - - - - - - - - - - - - 6C 2S Sep 04 19.1 Med 120-360 60 40 - - - - - - - - 0 6C Sep 10 13.4 Low 290-320 0 100 23 11 0 9C Sep 18 16.2 Med 160-380 20 80 25 12 800 24C Sep 25 16.6 Low 360 0 100 27 15 0 4C Oct 02 15.9 Low 320 0 100 24 12 0 5C Oct 09 n/a 0 0 0 0 24 11 500 1C Oct 16 13.3 0 0 0 0 24 11 800 1C Oct 23 12.6 0 0 0 0 28 14 1,600 2C Oct 31 10.4 0 0 0 0 37 22 1,600 3C Nov 06 9.2 0 0 0 0 34 18 0 0 Nov 14 7.2 0 0 0 0 33 18 4,900 0 Nov 27 3.6 0 0 0 0 33 17 1,600 0 The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 19, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet - 20 -

Date Water Temp Mussel larvae Size (microns) Chichester Cove Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 28 12.6 Low 90-110 100 0 - - - - - - - - - - - - - - - - June 04 17.6 Med 90-140 100 0 - - - - - - - - - - - - - - - - June 10 15.7 High 90-200 100 0 - - - - - - - - - - - - - - - - June 17 17.0 High 90-260 95 5 - - - - - - - - - - - - - - - - June 27 19.5 High 110-380 70 30 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 17, 2013-21 -

Date Water Temp Mussel larvae Size (microns) Covehead Bay Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae Apr 30 6.4 0 0 0 0 34 20 - - - - - - - - May 14 11.6 High 90-130 100 0 32 20 - - - - - - - - May 21 9.7 Med 110-140 100 0 33 20 - - - - - - - - May 27 12.1 High 110-160 100 0 29 16 - - - - - - - - June 03 15.3 High 90-180 100 0 24 13 - - - - - - - - June 10 13.4 High 100-250 98 2 24 13 - - - - - - - - June 17 15.6 High 100-260 90 10 23 12 - - - - - - - - June 24 20.2 High 90-300 80 20 25 13 - - - - - - - - July 02 17.5 High 110-320 60 40 24 13 - - - - - - - - July 08 23.5 Med 110-380 50 50 23 13 - - - - - - - - July 15 23.8 Med 110-340 40 60 25 13 - - - - - - - - July 22 21.7 Low 120-350 20 80 23 12 - - - - - - - - July 29 20.2 Low 120-180 100 0 24 12 - - - - - - - - Sep 05 18.2 - - - - - - - - - - - - - - - - - - - - - - - - 0 - - - - Sep 11 16.1 Med 120-360 50 50 31 17 0 - - - - Sep 24 16.1 Low 140-320 50 50 32 17 0 - - - - Sep 30 15.6 Low 130-150 100 0 - - - - - - - - 1,200 - - - - Oct 07 13.0 Low 140-180 100 0 30 15 200 - - - - Oct 15 12.1 0 0 0 0 30 15 700 - - - - Oct 22 11.5 0 0 0 0 29 16 15,200 - - - - Oct 28 7.9 0 0 0 0 30 15 13,300 - - - - Nov 05 6.5 0 0 0 0 33 16 16,200 - - - - Nov 12 5.8 0 0 0 0 32 18 10,700 - - - - Nov 19 6.5 0 0 0 0 33 18 1,000 - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 10, 2013-22 -

Date Water Temp Mussel larvae Size (microns) Darnley Basin Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) May 06 9.9 0 0 0 0 34 20 - - - - 0 May 28 11.2 0 0 0 0 - - - - - - - - - - - - 0 June 06 15.3 Med 90-120 100 0 25 13 - - - - 0 June 11 14.7 High 90-180 100 0 23 13 - - - - 0 June 20 18.0 High 90-240 100 0 24 12 - - - - 7S June 27 17.0 High 110-230 100 0 22 11 - - - - 9S July 04 19.4 High 90-260 90 10 22 11 - - - - 3S Tunicate Larvae July 12 21.4 High 110-340 60 40 20 10 - - - - 19S July 17 22.1 Med 110-360 50 50 19 10 - - - - 4S July 23 20.9 Low 110-380 50 50 20 10 - - - - 10S Aug 01 21.2 Low 110-220 100 0 19 9 - - - - 13S Aug 07 21.7 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 159S Aug 14 21.3 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 5S Aug 21 20.8 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 20S Sep 04 18.8 - - - - - - - - - - - - - - - - 18 9 0 2S Sep 10 15.0 Low 120-180 100 0 19 10 0 3S Sep 18 15.5 Low 120-140 100 0 20 11 0 1S Sep 24 16.0 Low 120 100 0 21 11 0 0 Sep 30 15.0 0 0 0 0 23 13 0 0 Oct 09 13.2 0 0 0 0 26 15 0 0 Oct 15 12.2 0 0 0 0 - - - - - - - - 3,000 0 Oct 23 11.1 0 0 0 0 28 15 956,000 0 Oct 31 6.8 0 0 0 0 25 14 9,600 0 Nov 04 7.0 0 0 0 0 26 15 12,300 0 Nov 13 3.3 0 0 0 0 26 15 9,800 0 The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately July 04, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet - 23 -

Date Water Temp Mussel larvae Size (microns) Pre-set Fox Island Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 27 11.2 0 0 0 0 - - - - - - - - - - - - - - - - June 03 14.8 0 0 0 0 - - - - - - - - - - - - - - - - June 11 15.7 Med 90-150 100 0 - - - - - - - - - - - - - - - - June 18 16.6 Med 90-240 100 0 - - - - - - - - - - - - - - - - June 24 18.1 Med 110-300 80 20 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 24, 2013 Date Water Temp Mussel larvae Size (microns) French River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunictae Larvae May 28 10.5 0 0 0 0 - - - - - - - - - - - - - - - - June 06 14.6 Low 90-170 100 0 - - - - - - - - - - - - - - - - June 11 13.2 High 90-200 100 0 - - - - - - - - - - - - - - - - June 20 16.6 Med 90-260 95 5 - - - - - - - - - - - - - - - - June 27 16.9 Med 110-360 80 20 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 20, 2013 Date Water Temp Mussel larvae Size (microns) Grand River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 28 12.7 0 0 0 0 - - - - - - - - - - - - - - - - June 04 16.7 Med 90-130 100 0 - - - - - - - - - - - - - - - - June 10 15.8 High 90-190 100 0 - - - - - - - - - - - - - - - - June 17 17.8 High 90-280 90 10 - - - - - - - - - - - - - - - - June 27 19.1 High 110-380 70 30 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 17, 2013-24 -

Date Water Temp Mussel larvae Size (microns) Hope River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 28 11.6 Med 110-160 100 0 - - - - - - - - - - - - - - - - June 06 15.2 Med 90-200 100 0 - - - - - - - - - - - - - - - - June 11 15.7 High 90-200 100 0 - - - - - - - - - - - - - - - - June 20 18.0 High 110-270 95 5 - - - - - - - - - - - - - - - - June 27 17.6 High 110-330 75 25 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 20, 2013 Date Water Temp Mussel larvae Size (microns) Hunter River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 28 11.1 Low 90-110 100 0 - - - - - - - - - - - - - - - - June 06 15.1 High 90-180 100 0 - - - - - - - - - - - - - - - - June 11 14.7 High 90-200 100 0 - - - - - - - - - - - - - - - - June 20 17.3 High 110-280 90 10 - - - - - - - - - - - - - - - - June 27 17.9 High 110-360 70 30 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 20, 2013 Date Water Temp Mussel larvae Size (microns) Kildare River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 27 12.1 Low 90-160 100 0 - - - - - - - - - - - - - - - - June 03 17.4 Low 90-190 100 0 - - - - - - - - - - - - - - - - June 11 15.6 High 90-130 100 0 - - - - - - - - - - - - - - - - June 18 17.7 High 90-220 100 0 - - - - - - - - - - - - - - - - June 24 22.4 High 90-280 90 10 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 24, 2013-25 -

Date Water Temp Mussel larvae Size (microns) Lennox Channel Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) May 06 10.6 0 0 0 0 33 19 - - - - 0 May 17 11.7 Low 90-110 100 0 29 16 - - - - 0 May 27 13.0 Low 90-140 100 0 26 14 - - - - 0 June 03 17.0 Low 90-190 100 0 27 15 - - - - 0 June 11 16.1 High 90-200 100 0 25 14 - - - - 0 June 18 17.2 High 100-260 95 5 29 17 - - - - 3S June 24 20.5 High 110-320 80 20 24 13 - - - - 3S July 05 19.1 High 110-320 50 50 23 14 - - - - 3S July 17 23.3 Med 110-380 50 50 20 11 - - - - 3S July 26 21.2 Low 110-380 50 50 20 11 - - - - 2S Aug 07 21.4 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 6S Aug 14 21.8 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1S Aug 21 22.2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 5S Aug 29 20.0 Low 130-380 50 50 - - - - - - - - - - - - 3S Sep 04 18.7 - - - - - - - - - - - - - - - - 21 13 0 4S Sep 10 15.0 Low 130 100 0 22 12 0 1S Sep 18 16.0 0 0 0 0 24 12 0 0 Sep 24 16.0 0 0 0 0 26 14 0 0 Sep 30 14.0 0 0 0 0 25 14 0 0 Oct 16 12.2 0 0 0 0 28 16 58,000 0 Oct 23 11.6 0 0 0 0 26 15 3,240,000 0 Nov 06 6.9 0 0 0 0 26 15 396,000 0 Nov 13 4.6 0 0 0 0 ---- ---- 240,000 0 The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 18, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet Tunicate Larvae - 26 -

Date Water Temp Mussel larvae Size (microns) March Water Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) May 28 13.0 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0 June 04 15.7 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0 June 10 14.5 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1S Tunicate Larvae June 17 16.4 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 19S June 27 18.6 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 20S July 04 20.5 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 45S July 12 22.7 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 8S July 23 22.4 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3S Aug 01 21.6 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3S Aug 07 21.7 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 126S Aug 14 21.4 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 7S Aug 21 21.4 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 144S Sep 04 18.8 - - - - - - - - - - - - - - - - 20 12 - - - - 2S Sep 10 15.0 - - - - - - - - - - - - - - - - 20 12 - - - - 3S Sep 18 15.0 - - - - - - - - - - - - - - - - 20 12 - - - - 0 Sep 24 16.1 - - - - - - - - - - - - - - - - 24 14 700 2S Sep 30 15.2 - - - - - - - - - - - - - - - - 25 16 - - - - 0 Oct 16 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 38,200 2S Oct 23 11.7 - - - - - - - - - - - - - - - - - - - - - - - - 2,760,000 0 Oct 31 6.5 - - - - - - - - - - - - - - - - 29 15 164,000 0 Nov 06 6.7 - - - - - - - - - - - - - - - - 29 17 287,000 0 The setting of mussel larvae occurs when they reach a size of at least 250 microns. C= Ciona S=Styela GS=Goldenstar V=Violet - 27 -

Date Water Temp Mussel larvae Size (microns) Pre-set Mill River Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 27 12.4 0 0 0 0 - - - - - - - - - - - - - - - - June 03 15.2 0 0 0 0 - - - - - - - - - - - - - - - - June 11 15.4 Med 90-180 100 0 - - - - - - - - - - - - - - - - June 18 16.9 High 90-260 90 10 - - - - - - - - - - - - - - - - June 24 19.1 High 90-310 80 20 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 18, 2013 Date Water Temp Mussel larvae Size (microns) Pre-set Montrose Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 27 12.1 Low 90-140 100 0 - - - - - - - - - - - - - - - - June 03 18.0 Low 90-180 100 0 - - - - - - - - - - - - - - - - June 11 18.4 Med 90-140 100 0 - - - - - - - - - - - - - - - - June 18 17.9 Med 90-220 100 0 - - - - - - - - - - - - - - - - June 24 23.4 High 90-320 80 20 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 24, 2013. - 28 -

Date Water Temp Mussel larvae Size (microns ) Murray River Pre-set Setsize Steamed Meat Yield European Meat Yield #Pseudonitzschia (cells/l) May 02 9.4 0 0 0 0 29 16 - - - - 0 May 15 9.2 Low 90-110 100 0 22 13 - - - - 0 May 24 11.1 0 0 0 0 42 25 - - - - 0 May 30 11.7 Low 90-110 100 0 34 20 - - - - 0 Tunicate Larvae Jun 05 13.3 High 90-180 100 0 31 17 - - - - 4C Jun 13 13.3 High 90-210 100 0 33 20 - - - - 4C 2S Jun 18 14.6 High 110-210 95 5 30 15 - - - - 2C 1S Jun 26 17.3 High 90-300 80 20 29 15 - - - - 4C 3S Jul 03 18.8 High 110-330 80 20 28 14 - - - - 2C Jul 10 22.0 High 110-340 60 40 28 14 - - - - 3C 2S Jul 18 22.3 Med 110-380 50 50 24 12 - - - - 3C 4S Jul 24 21.4 Low 120-380 50 50 24 12 - - - - 11C 12S Jul 30 21.1 Low 120-380 50 50 24 12 - - - - 4C 1S Aug 05 22.1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 5C 4S Aug 13 20.6 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1C 3S Aug 20 21.1 Low 120-190 100 0 - - - - - - - - - - - - 32C 2S 1GS Aug 27 18.9 Low 120-180 100 0 - - - - - - - - - - - - 22C 1S Sep 04 19.3 Low 120-330 50 50 18 9 0 44C 4S Sep 09 16.2 Low 280-360 100 0 19 9 0 117C 3S Sep 17 16.3 Low 120-190 100 0 19 10 0 200C 4S Sep 25 16.2 0 0 0 0 21 10 0 29C Oct 03 15.9 0 0 0 0 19 9 0 28C Oct 10 14.3 0 0 0 0 20 10 0 6C Oct 16 12.7 0 0 0 0 19 9 0 2C 1S Oct 23 12.6 0 0 0 0 22 11 100 3C Oct 31 8.2 0 0 0 0 20 10 0 2C Nov 06 8.9 0 0 0 0 20 10 0 0 The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 18, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet - 29 -

Date Water Temp Mussel larvae Size (microns) New London Bay Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 03 8.8 0 0 0 0 29 17 - - - - - - - - May 14 11.5 Med 90-110 100 0 33 19 - - - - - - - - June 06 15.0 Med 90-180 100 0 31 17 - - - - - - - - June 11 14.8 High 90-200 100 0 28 17 - - - - - - - - June 20 17.7 High 90-260 95 5 27 17 - - - - - - - - June 27 17.8 High 110-340 80 20 25 14 - - - - - - - - July 04 19.3 Med 110-300 50 50 24 13 - - - - - - - - July 12 21.4 Med 110-360 50 50 21 11 - - - - - - - - July 23 20.7 Med 110-370 50 50 22 12 - - - - - - - - Sep 04 18.6 - - - - - - - - - - - - - - - - 18 10 - - - - - - - - Sep 10 15.5 Low 110-130 100 0 20 11 0 - - - - Sep 18 15.8 Low 120-160 100 0 18 9 0 - - - - Sep 24 15.9 0 0 0 0 17 9 400 - - - - Sep 30 15.8 0 0 0 0 19 10 0 - - - - Oct 07 13.5 0 0 0 0 28 16 2,000 - - - - Oct 16 n/a 0 0 0 0 26 14 17,700 - - - - Oct 24 11.2 0 0 0 0 24 13 67,400 - - - - Oct 31 7.5 0 0 0 0 26 16 593,00 - - - - Nov 04 7.2 0 0 0 0 29 19 117,800 - - - - Nov 13 4.5 0 0 0 0 - - - - - - - - 156,000 - - - - Nov 18 5.1 0 0 0 0 34 23 31,800 - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 20, 2013-30 -

Date Water Temp Mussel larvae Size (microns) Nine Mile Creek Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 29 10.2 Low 90-110 100 0 - - - - - - - - - - - - - - - - June 05 16.7 High 90-180 100 0 - - - - - - - - - - - - - - - - June 12 12.9 High 90-230 100 0 - - - - - - - - - - - - - - - - June 19 15.2 High 90-280 90 5 - - - - - - - - - - - - - - - - June 25 15.5 High 110-320 85 15 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 19, 2013 Date Water Temp Mussel larvae Size (microns) North Lake Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 31 9.9 Low 90-110 100 0 - - - - - - - - - - - - - - - - June 07 12.8 Med 90-140 100 0 - - - - - - - - - - - - - - - - June 14 12.0 Med 90-200 100 0 - - - - - - - - - - - - - - - - June 21 15.7 High 90-220 100 0 - - - - - - - - - - - - - - - - June 28 16.5 High 110-260 95 5 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 28, 2013 Date Water Temp Mussel larvae Size (microns) Pre-set Orwell Bay Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 29 11.6 Low 110-120 100 0 - - - - - - - - - - - - - - - - June 05 14.0 High 90-200 100 0 - - - - - - - - - - - - - - - - June 12 13.5 High 90-220 100 0 - - - - - - - - - - - - - - - - June 19 17.1 Med 90-290 95 5 - - - - - - - - - - - - - - - - June 25 19.3 High 110-330 85 15 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 19, 2013-31 -

Date Water Temp Mussel larvae Size (microns) Rustico Bay Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 03 8.1 0 0 0 0 29 16 - - - - - - - - May 14 12.7 Med 90-110 100 0 29 17 - - - - - - - - May 28 12.1 Med 90-120 100 0 26 14 - - - - - - - - June 11 16.0 High 90-220 100 0 28 16 - - - - - - - - June 20 17.8 High 110-260 90 10 26 13 - - - - - - - - June 27 17.8 High 110-330 75 25 24 13 - - - - - - - - July 04 20.0 Med 110-320 50 50 27 15 - - - - - - - - July 11 22.5 Med 110-360 50 50 22 13 - - - - - - - - Sep 05 18.2 - - - - - - - - - - - - - - - - 24 12 0 - - - - Sep 10 15.0 0 0 0 0 24 14 0 - - - - Sep 18 18.0 0 0 0 0 26 15 0 - - - - Sep 24 16.0 0 0 0 0 25 14 0 - - - - Oct 02 15.4 0 0 0 0 19 10 0 - - - - Oct 07 12.6 0 0 0 0 20 11 1,100 - - - - Oct 24 10.9 0 0 0 0 29 18 9,600 - - - - Oct 31 5.9 0 0 0 0 22 11 5,600 - - - - Nov 04 7.1 0 0 0 0 - - - - - - - - 21,900 - - - - Nov 13 - - - - - - - - - - - - - - - - - - - - 29 18 - - - - - - - - Nov 18 4.6 0 0 0 0 - - - - - - - - 3,700 - - - - Nov 27 0.1 0 0 0 0 24 14 300 - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 20, 2013-32 -

Date Water Temp Mussel larvae Size (microns) Savage Harbour Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) May 01 4.9 0 0 0 0 27 15 - - - - 0 May 13 9.8 0 0 0 0 26 15 - - - - 0 May 22 8.5 0 0 0 0 26 15 - - - - 0 May 27 10.0 Low 90-110 100 0 23 14 - - - - 0 June 03 14.7 Med 90-170 100 0 24 13 - - - - 0 June 10 12.3 Med 90-120 100 0 23 13 - - - - 0 June 17 14.8 High 90-250 98 2 23 12 - - - - 0 June 24 18.7 Med 90-260 90 10 22 11 - - - - 0 July 02 18.5 Med 110-320 80 20 22 12 - - - - 0 July 08 21.1 Med 110-340 60 40 20 10 - - - - 0 July 15 22.0 High 110-360 50 50 20 11 - - - - 0 July 22 21.6 Med 110-340 30 70 19 10 - - - - 0 July 29 18.9 Low 110-330 50 50 19 11 - - - - 0 Sep 05 17.2 - - - - - - - - - - - - - - - - 28 12 0 0 Sep 11 15.6 Low 100 100 0 23 12 0 0 Sep 16 16.5 Low 100 100 0 26 14 0 0 Sep 24 15.2 Low 100 100 0 26 15 0 0 Sep 30 15.3 0 0 0 0 26 13 0 0 Oct 09 12.3 0 0 0 0 27 14 700 0 Oct 15 12.0 0 0 0 0 28 15 3,100 0 Oct 22 11.2 0 0 0 0 26 14 1,500 0 Oct 28 8.2 0 0 0 0 32 17 4,900 0 Nov 05 7.6 0 0 0 0 30 16 94,200 0 Nov 12 6.9 0 0 0 0 30 17 25,100 0 Nov 19 7.4 0 0 0 0 30 17 10,900 0 Nov 27 1.0 0 0 0 0 28 14 3,300 0 The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 17, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet Tunicate Larvae - 33 -

Date Water Temp Mussel larvae Size (microns) Souris River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 31 9.6 0 0 0 0 - - - - - - - - - - - - - - - - June 07 10.3 Low 90-130 100 0 - - - - - - - - - - - - - - - - June 14 11.3 Low 90-160 100 0 - - - - - - - - - - - - - - - - June 21 14.5 Med 90-210 100 0 - - - - - - - - - - - - - - - - June 28 14.6 Med 90-250 98 2 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 28, 2013 Date Water Temp Mussel larvae Size (microns) Southwest River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 28 8.2 0 0 0 0 - - - - - - - - - - - - - - - - June 06 15.4 Low 90-180 100 0 - - - - - - - - - - - - - - - - June 11 14.6 High 90-210 100 0 - - - - - - - - - - - - - - - - June 20 18.8 Med 90-280 95 5 - - - - - - - - - - - - - - - - June 27 16.4 Med 110-320 80 20 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 20, 2013 Date Water Temp Mussel larvae Size (microns) Stanley River Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 28 11.3 Low 110-140 100 0 - - - - - - - - - - - - - - - - June 06 15.4 Med 90-200 100 0 - - - - - - - - - - - - - - - - June 11 15.2 High 90-250 98 2 - - - - - - - - - - - - - - - - June 20 18.6 High 110-280 90 10 - - - - - - - - - - - - - - - - June 27 18.0 High 110-360 70 30 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 11, 2013-34 -

Date Water Temp Mussel larvae Size (microns) St. Mary s Bay Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) May 09 10.3 0 0 0 0 42 27 - - - - 0 May 16 8.8 Low 90-120 100 0 41 27 - - - - 0 May 24 9.1 Low 110 100 0 43 27 - - - - 0 May 30 10.1 Low 90-110 100 0 40 25 - - - - 0 June 18 14.7 High 90-260 95 5 32 18 - - - - 0 Tunicate Larvae June 26 16.8 High 90-380 90 10 31 16 - - - - 3C July 03 18.5 High 110-280 80 20 29 15 - - - - 2C 1S July 10 21.1 Med 110-340 50 50 31 17 - - - - 3C 3S July 18 22.7 Med 110-400 50 50 32 18 - - - - 6C 4S July 24 20.1 Low 110-320 50 50 - - - - - - - - - - - - 2C 3S July 30 21.2 Low 110-200 100 0 - - - - - - - - - - - - 4C 5S Aug 05 21.8 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3S Aug 13 21.0 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2S Aug 20 19.8 Med 120-360 80 20 - - - - - - - - - - - - 5C 2S Aug 27 19.6 Low 110-130 100 0 - - - - - - - - - - - - 3C 2S Sep 04 18.9 Low 320-360 0 100 26 13 0 6C 2S Sep 18 15.9 Med 200-340 10 90 24 12 1,000 14C Sep 25 16.2 Low 260-320 100 0 26 14 0 9C 2S Oct 10 13.4 0 0 0 0 27 13 0 2C Oct 16 12.3 0 0 0 0 26 13 0 2C Oct 23 12.2 0 0 0 0 25 12 0 2C Oct 31 7.5 0 0 0 0 24 12 0 0 Nov 06 7.7 0 0 0 0 26 13 0 0 Nov 14 5.0 0 0 0 0 26 14 1,600 0 The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 18, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet - 35 -

Date Water Temp Mussel larvae Size (microns) St. Peter s Bay Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 01 5.9 0 0 0 0 38 22 - - - - - - - - May 13 12.6 Low 90-110 100 0 31 18 - - - - - - - - May 22 10.0 Low 110-140 100 0 32 17 - - - - - - - - May 27 12.2 Med 90-120 100 0 26 14 - - - - - - - - June 04 13.4 High 90-200 100 0 27 13 - - - - - - - - June 12 14.3 High 90-240 100 0 - - - - - - - - - - - - - - - - June 17 15.7 High 90-260 90 10 26 13 - - - - - - - - June 24 18.2 Med 90-320 80 20 24 12 - - - - - - - - July 02 18.7 Med 90-300 70 30 24 11 - - - - - - - - July 09 22.3 High 110-300 50 50 23 11 - - - - - - - - July 15 23.2 High 110-380 30 70 21 10 - - - - - - - - July 22 21.5 Low 110-360 50 50 21 10 - - - - - - - - July 29 20.9 Low 320 0 100 20 10 - - - - - - - - Sep 05 18.3 - - - - - - - - - - - - - - - - 22 10 0 - - - - Sep 12 16.8 Low 320 0 100 23 11 0 - - - - Sep 16 16.5 Low 120-200 100 0 22 10 0 - - - - Sep 24 16.2 Low 140 100 0 25 13 0 - - - - Sep 30 15.6 Low 130-160 100 0 21 9 200 - - - - Oct 09 13.0 0 0 0 0 26 13 400 - - - - Oct 15 12.0 0 0 0 0 25 12 700 - - - - Oct 22 11.7 0 0 0 0 26 13 1,100 - - - - Oct 30 7.8 0 0 0 0 35 20 1,600 - - - - Nov 05 7.7 0 0 0 0 32 16 3,000 - - - - Nov 12 6.0 0 0 0 0 33 17 7,000 - - - - Nov 19 6.1 0 0 0 0 33 17 4,300 - - - - Nov 27 0.7 0 0 0 0 32 17 500 - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 17, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet - 36 -

Date Water Temp Mussel larvae Size (microns) Tracadie Bay Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae Apr 30 6.8 0 0 0 0 31 18 - - - - - - - - May 13 12.0 Med 90-140 100 0 35 21 - - - - - - - - May 21 8.8 Med 90-120 100 0 37 23 - - - - - - - - May 27 11.5 Med 90-150 100 0 36 22 - - - - - - - - June 10 14.0 High 90-240 100 0 24 12 - - - - - - - - June 17 15.3 High 90-270 90 10 26 14 - - - - - - - - June 24 19.0 Med 90-280 90 10 23 12 - - - - - - - - July 08 23.1 Med 110-360 40 60 24 13 - - - - - - - - July 15 23.1 Med 110-360 30 70 22 11 - - - - - - - - July 22 21.8 Med 110-360 30 70 22 11 - - - - - - - - July 29 20.6 Low 110-360 50 50 - - - - - - - - - - - - - - - - Sep 05 17.9 - - - - - - - - - - - - - - - - 21 11 0 - - - - Sep 11 15.8 Low 110-160 100 0 20 11 0 - - - - Sep 16 16.0 Low 110-140 100 0 20 10 0 - - - - Sep 24 16.3 0 0 0 0 22 12 0 - - - - Sep 30 15.2 Low 120-140 100 0 22 11 2,200 - - - - Oct 07 13.1 Low 120-160 100 0 24 12 200 - - - - Oct 15 12.1 0 0 0 0 24 12 0 - - - - Oct 22 11.4 0 0 0 0 24 14 5,200 - - - - Oct 28 8.8 0 0 0 0 26 13 8,500 - - - - Nov 05 6.7 0 0 0 0 28 14 10,200 - - - - Nov 12 6.0 0 0 0 0 26 13 2,800 - - - - Nov 19 6.4 0 0 0 0 24 13 900 - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 17, 2013 C= Ciona S=Styela GS=Goldenstar V=Violet - 37 -

Date Water Temp Mussel larvae Size (microns) Webber Cove Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 28 12.3 Low 90-140 100 0 - - - - - - - - - - - - - - - - June 04 16.4 Low 90-120 100 0 - - - - - - - - - - - - - - - - June 10 15.5 High 90-210 100 0 - - - - - - - - - - - - - - - - June 17 17.1 High 90-250 95 5 - - - - - - - - - - - - - - - - June 27 19.6 High 110-310 70 30 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 17, 2013 Date Water Temp Mussel larvae Size (microns) Winter Bay Pre-set Set-size Steamed European #Pseudonitzschia (cells/l) Tunicate Larvae May 27 12.4 High 90-160 100 0 - - - - - - - - - - - - - - - - June 03 16.0 High 90-200 100 0 - - - - - - - - - - - - - - - - June 10 14.5 High 90-250 98 2 - - - - - - - - - - - - - - - - June 17 15.8 High 90-260 90 10 - - - - - - - - - - - - - - - - June 24 19.4 High 90-280 80 20 - - - - - - - - - - - - - - - - July 02 19.7 Med 110-340 50 50 - - - - - - - - - - - - - - - - The setting of mussel larvae occurs when they reach a size of at least 250 microns. Setting started in this area approximately June 10, 2013-38 -

APPENDIX II Maps of Sample Sites - 39 -

OB-7273-L BOT-7256-L BOT-6599-L BOT-7482-L BOT-5420-L OB-7319-L OB-6968-L SPAT-0183B SPAT-0184B SEED-0564 OB-2934-L OB-3216-L SPAT-0183A SEED-0563 Mussel Monitoring Sampling Site SEED-0519 Baltic River Mussel Monitoring Site BOT-7174-L OB-6907-L OB-7585-L BOT-7534-L SPAT-0403 SPAT -0332 SUR-0720-L OB-7010-L SPAT -0323 SPAT -0336 SUR-0712-L SUR-0729-L Mussel Monitoring Sampling Sites SPAT -0275 SPAT -0291 SUR-0645-L BOT -7739-L SPAT-0180A SPAT-0180B SPAT -0368 SPAT -0292 BOT-7463-L SPAT-0271 SPAT-0276 BOT-4000-L SPAT-0219 Bentick Cove, Webber Cove and Chichester Cove Mussel Monitoring Site - 40 -

SPAT-0398 BOT-7483-L OB-7557-L SEED-0418 OB-7640-L SUR-0461-L SUR-0539-L SUR-0754-L SUR-0582-L SUR-0755-L SUR-0072-L SUR-0522-L SUR-0165-L BOT-7760-L BOT-7761-L BOT-7722-L Mussel Monitoring Sampling Site Boughton River Mussel Monitoring Site BOT-7763-L SUR-0707-L SUR-0464-L SUR-0048-L BOT-7572-L SUR-0762-L SUR-0052-L SUR-0278-L SUR-0696-L SUR-0697-L SUR-0636-L SUR-0656-L SUR-0731-L SUR-0698-L SUR-0080-L SUR-0590-L SUR-0732-L SUR-0532-L SUR-0710-L SPAT-0393 SUR-0100-L SUR-0738-L Mussel Monitoring Sampling Site SUR-0728-L Brudenell River Mussel Monitoring Site - 41 -

SUR-0117B-L SUR-0117A-L SUR-0242-L SUR-0121-L OB-7479-L OB-7480-L Mussel Monitoring Sampling Site SUR-0706-L SUR-0621-L SUR-0632-L SUR-0671-L SUR-0640-L BOT-7763-L SUR-0707-L Cardigan River Mussel Monitoring Site BOT-7510-L BOT-7511-L BOT-7723-L BOT-7514-L BOT-7642-L SUR-0554-L Mussel Monitoring Sampling Site SUR-0638-L Covehead Bay Mussel Monitoring Site - 42 -

BOT-6664-L SUR-0523-L BOT-6732-L BOT-7556-L SUR-0524-L SUR-0624-L BOT-6657-L BOT-7748-L OB-7538-L OB-7405-L BOT-7035-L BOT-2350-L SUR-0625-L SUR-0675-L SUR-0591-L BOT-6407-L SUR-0596-L SUR-0595-L SUR-0589-L BOT-7070-L Mussel Monitoring Sampling Site BOT-7250-L SUR-0592-L OB-7272-L SUR-0616-L SUR-0594-L OB-7264-L BOT-7207-L OB-7330-L OB-7273-L BOT-7256-L Darnley Basin Mussel Monitoring Site SEED-0375 Mussel Monitoring Sampling Site SEED-0371 SEED-0031 SEED-0152 French River Mussel Monitoring Site - 43 -

BOT-6965-L BOT-7704-L OB-6725-L OB-7385-L BOT -6285-L BOT -6274-L SPAT -0315 SPAT-0284 BOT -5092-L Mussel Monitoring Sampling Site BOT-7181-L BOT-6920-L OB-6765-L BOT-3665-L Grand River Mussel Monitoring Site BOT-4273-L BOT-7565-L BOT-7566-L OB-7565-L OB-7566-L BOT-7736-L BOT-7735-L OB-7567-L SPAT-0288 SPAT-0289 Mussel Monitoring Sampling Site SEED-0523B SEED-0370 SEED-0523A L Hope River Mussel Monitoring Site - 44 -

SUR-0677-L BOT-6793-L SUR-0658-L SUR-0612-L SUR-0676-L SUR-0687-L SUR-0545-L SUR-0546-L Mussel Monitoring Sampling Site SPAT-0321 Hunter River Mussel Monitoring Site SPAT -0278 Mussel Monitoring Sampling Sites SPAT-0314 SEED-0524 SPAT-0331 OB-7578-L BOT -7136-L BOT-7135-L Kildare River and Montrose Mussel Monitoring Sites - 45 -

BOT-5858-L OB-7611-L BOT-6939-L BOT-7365-L OB-7316-L BOT-6685-L 562-L 8-L 653-L OB-7318-L OB-7224-L BOT-7387-L BOT-7549-L 741-L SPAT-0213 BOT-7743-L T-5324-L BOT-7046-L OB-6555-L BOT-7403-L OB-6270-L BOT-7563-L BOT-7077-L BOT-6751-L BOT-6802-L BOT-7717-L BOT-7506-L BOT-7142-L BOT-4526-L Mussel Monitoring Sampling Site BOT-7056-L BOT-7377-L BOT-4418-L -7078-L 389-L -L 7703-L BOT-7550-L BOT-7364-L OB-7205-L SUR-0536-L SUR-0200-L SUR-0540-L Lennox Channel Mussel Monitoring Site Mussel Monitoring Sampling Site SUR-0527-L SUR-0525-L SUR-0526-L SUR-0529-L BOT-7335-L BOT-7173-L BOT-7201-L BOT-7183-L SUR-0614-L SUR-0558-L OB-7141-L OB-7546-L SUR-0528-L BOT-7487-L SUR-0759-L L 72-L SUR-0553-L SUR-0637-L T-7466-L BOT-7651-L BOT-7353-L SUR-0584-L SUR-0530-L SUR-0531-L BOT-7641-L BOT-6921-L BOT-6459-L BOT-6877-L BOT-7351-L SUR-0650-L BOT-7174-L March Water Mussel Monitoring Site - 46 -

BOT-6805-L OB-7371-L OB-7631-L BOT -7453-L BOT-5427-L OB-7376-L BOT-7523-L OB-7196-L OB-6986-L BOT-3431-L BOT-3617-L OB-7762-L BOT-5443-L SPAT-0246 SPAT-0313 BOT-7043-L BOT-7060-L OB-7271-L SPAT-0353 BOT-6974-L OB-6914-L Mussel Monitoring Sampling Sites BOT -6355-LBOT-7374-L BOT -7781-L OB-2861-L OB-7777-L BOT-7278-L BOT-7277-L BOT-7669-L SUR-0690-L BOT-7279-L BOT-2852-L SUR-0694-L Mill River and Fox Island Mussel Monitoring Sites OB-6841-L SUR-0145-L SEED-0269 SUR-0151-L SUR-0302-L SUR-0753-L SUR-0520-L OB-7574-L SUR-0129-L BOT-5763-L SUR-0152-L SUR-0685-L BOT-7573-L SUR-0704-L Mussel Monitoring Sampling Site SUR-0158-L SUR-0581-L SUR-0684-L SUR-0757-L OB-5448-L BOT-5422-L SUR-0171-L BOT-5293-L SPAT-0408 SPAT-0312 Murray River Mussel Monitoring Site - 47 -

SUR-0503B-L 7259-L 9-L SUR-0510-L SUR-0509-L SUR-0508-L SUR-0507-L SUR-0758-L SUR-0506-L Mussel Monitoring Sampling Site BOT-7268-L BOT-7522-L 7-L T-7633-L SUR-0504-L SUR-0505-L L 82-L SUR-0517-L SUR-0503A-L BOT-7577-L BOT-7565-L OB-7565-L OB-7566-L OB-7567-L SPAT-0288 Mussel Monitoring Sampling Site SPAT-0289 New London Mussel Monitoring Site SUR-0725-L Mussel Monitoring Sampling Site SUR-0727-L SUR-0717-L SUR-0746-L SUR-0723-L SUR-0743-L SUR-0726-L SUR-0713-L SUR-0763-L SUR-0736-L SUR-0715-L BOT-7645-L BOT-7647-L BOT-7604-L SPAT-0366 BOT-7701-L BOT-7724-L Nine Mile Creek Mussel Monitoring Site - 48 -

Mussel Monitoring Sampling Site SUR-0721-L SUR-0722-L SUR-0718-L North Lake Mussel Monitoring Site BOT-7469-L BOT-4475-L BOT-6711-L BOT-7186-L BOT-7465-L BOT-7293-L BOT-7495-L BOT-3948-L OB-7489-L BOT-7493-L BOT-7322-L SUR-0699-L SUR-0769-L SUR-0646-L SUR-0768-L SUR-0767-L Mussel Monitoring Sampling Site Orwell Bay Mussel Monitoring Site - 49 -

BOT-7524-L Mussel Monitoring Sampling Site BOT-7570-L SUR-0550-L SUR-0549-L SUR-0551-L SUR-0547-L SUR-0695-L SUR-0552-L SUR-0689-L SUR-0548-L SUR-0674-L SUR-0634-L Rustico Bay Mussel Monitoring Site Bot-7603-L BOT-7683-L Mussel Monitoring Sampling Site BOT-7464-L SUR-0631-L SUR-0604-L SUR-0603-L SUR-0602-L BOT-7733-L SPAT-0234 BOT-4830-L SUR-0601-L SUR-0609-L SUR-0691-L SUR-0608-L SUR-0607-L SUR-0598-L SUR-0606-L SUR-0605-L OB-7270-LBOT-6054-L SUR-0660-L SUR-0678-L Savage Harbour Mussel Monitoring Site - 50 -

SUR-0683-L SUR-0659-L Mussel Monitoring Sampling Site BOT -7530-L BOT-7529-L BOT-7532-L Souris River Mussel Monitoring Site OB-7569-L SUR-0337-L BOT-7633-L Mussel Monitoring Sampling Site SUR-0221-L BOT-7755-L BOT-7075-L SUR-0268-L BOT-7541-L SUR-0716-L BOT-6668-L OB-7305-L SPAT-0277 SUR-0682-L BOT-7630-L BOT-7542-L BOT-3122-L BOT-7681-L BOT-7537-L SUR-0339-L BOT-6203-L BOT-6380-L BOT-4161-L OB-7434-L BOT-4737-L Southwest River Mussel Monitoring Site - 51 -

BOT-5264-L BOT-5686-L BOT-6667-L BOT-6579-L BOT-6201-L BOT-6718-L SUR-0583-L Mussel Monitoring Sampling Site SEED-0257 Stanley River Mussel Monitoring Site SUR-0711-L SUR-0739-L BOT-7543-L BOT-7699-L SUR-0705-L BOT-7545-L BOT-7752-L Mussel Monitoring Sampling Site SUR-0064-L SUR-0709-L SUR-0644-L SUR-0618-L SUR-0619-L SUR-0643-L SUR-0450-L SUR-0770-L SUR-0008-L SUR-0741-L SUR-0579-L SUR-0641-L BOT-7544-L BOT-7737-L BOT-7680-L St. Mary s Bay Mussel Monitoring Site - 52 -

SUR-0680-L SUR-0193-L SUR-0585-L SUR-0538-L SUR-0521A-L SUR-0535-L SUR-0310-L OB-7424-L SUR-0533-L SUR-0663-L SUR-0188-L Mussel Monitoring Sampling Site SUR-0700-L SUR-0214-L SUR-0702-L SUR-0630-L SUR-0120-L SUR-0147-L SUR-0183-L SUR-0174-L SEED-0172 St. Peter s Bay Mussel Monitoring Site SUR-0563-L SUR-0662-L SUR-0385-L BOT-7552-L BOT-4815-L BOT-7536-L SUR-0500-L SUR-0537-L SUR-0334-L SUR-0279-L SUR-0670-L SUR-0543-L SUR-0384-L BOT-7048-L SUR-0708-L SUR-0055-L SUR-0574-L SUR-0564-L BOT-6973-L SUR-0541-L SUR-0760-L SUR-0655-L SUR-0693-L BOT-7581-L SUR-0136A-L BOT-7050B-L Mussel Monitoring Sampling Sites Mussel Monitoring Sampling Sites SUR-0692-L OB-7580-L SUR-0733-L Tracadie Bay and Winter Bay Mussel Monitoring Sites - 53 -

APPENDIX III Water Temperature Graphs for Sample Sites - 54 -

Temperature data collected at Boughton River Mussel Monitoring Station 2013 Temperature data collected at Brudenell River Mussel Monitoring Station 2013-55 -

Temperature data collected at Covehead Bay Mussel Monitoring Station 2013 Temperature data collected at Darnley Basin Mussel Monitoring Station 2013-56 -

Temperature data collected at Lennox Channel Mussel Monitoring Station 2013 Temperature data collected at Murray River Mussel Monitoring Station 2013-57 -

Temperature data collected at New London Bay Mussel Monitoring Station 2013 Temperature data collected at Rustico Bay Mussel Monitoring Station 2013-58 -

Temperature data collected at Savage Harbour Mussel Monitoring Station 2013 Temperature data collected at St. Mary s Bay Mussel Monitoring Station 2013-59 -

Temperature data collected at St. Peter s Bay Mussel Monitoring Station 2013 Temperature data collected at Tracadie Bay Mussel Monitoring Station 2013-60 -

APPENDIX IV Mussel Steamed Information - 61 -

Comparison of average steamed meat yields from mussels collected from 14 Mussel Monitoring Sites in 2009, 2010, 2011, 2012 and 2013. Area 2009 2010 2011 2012 2013 Boughton River 29.6 32.8 29.6 28.1 32.5 Brudenell River 29.6 35.5 35.9 35.2 32.6 Cardigan River 34.2 32.0 32.5 33.8 29.4 Covehead Bay 30.5 37.8 34.2 37.5 28.3 Darnley Basin 27.7 24.0 26.9 24.0 22.8 Lennox Channel 33.1 29.3 27.9 24.7 25.2 March Water 25.6 24.9 25.0 24.4 23.8 Murray River 24.9 23.7 24.1 23.5 25.0 New London Bay 30.3 27.2 29.1 27.4 24.8 Rustico Bay 31.1 31.0 27.0 26.3 25.2 Savage Harbour 28.5 23.6 27.0 26.8 25.1 St. Mary s Bay 30.6 30.9 29.0 32.8 30.6 St. Peter s Bay 27.5 25.4 27.0 24.1 27.1 Tracadie Bay 24.4 26.2 25.0 25.0 25.7-62 -

Comparison of average meat weight from mussels collected from 14 Mussel Monitoring Sites in 2013. Area Minimum (g) Maximum (g) Average (g) Boughton River 1.0 5.1 2.5 Brudenell River 1.8 5.6 3.7 Cardigan River 1.4 3.3 2.5 Covehead Bay 2.3 4.6 3.1 Darnley Basin 1.3 3.6 2.4 Lennox Channel 1.1 5.0 2.8 March Water 1.6 3.0 2.1 Murray River 2.2 5.9 3.2 New London Bay 1.4 4.1 2.4 Rustico Bay 1.0 2.9 1.9 Savage Harbour 1.5 3.8 2.3 St. Mary s Bay 1.6 4.9 3.1 St. Peter s Bay 1.9 4.9 2.9 Tracadie Bay 1.9 5.1 2.9-63 -

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APPENDIX V Maps Displaying Current Known Range of Invasive Tunicates in PEI - 71 -

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