C. Area of operation: Columbia River and Columbia River Estuary between mouth of estuary and Portland, Oregon.

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R/V Barnes Cruise Plan July 2008 Cruise dates: July 7 24 Captain: Ray McQuin Lead scientist: Joseph Needoba (needobaj@stccmop.org) Funding source: NSF PI Antonio Baptista Organization: NSF Center for Coastal Margin Observation & Prediction A. Science team Participants: Leg 1 July 9 15 1) Joseph Needoba (OHSU) 2) Melissa Rohde (OHSU) 3) Florian Moeller (OHSU) 4) Julia Schoen (OHSU/Cornell) 5) Jami Goldman (OHSU) 6) Jim Postel (UW) Leg 2 July 16 22 1) Joseph Needoba (OHSU) 2) David Needham (OHSU) 3) Mariya Smit (OHSU) 4) Kaitlin Tyrol (OHSU) 5) Shana Radford (OHSU) 6) Jim Postel (UW) B. Purpose of mission: 1) Evaluate CMOP ocean observatory infrastructure and instrumentation. Ground truth SATURN 01 and 03 stations and instrumentation. 2) Collect samples for microbial ecology investigations (extension of 2007 sampling program) 3) Biogeochemistry measurements across river to ocean domain 4) Adaptive sampling exercises to measure salt intrusion and ETM dynamics C. Area of operation: Columbia River and Columbia River Estuary between mouth of estuary and Portland, Oregon. D. Major Equipment: 1) CTD with fluorometer, transmissometer, oxygen electrode, ph (University of Washington) and variable fluorescence fluorometer (OHSU) 2) Pacer S series electric pump with 100 ft 1 1 2 pvc pump hose 3) On deck incubator plumbed to surface water flow through system 4) Freezer 5) ADCP 6) Bacterial productivity incubator E. Radioactivity usage 3 H bacterial productivity measurements (Mariya Smit)

F. Approximate tides: July 7 transit July 9 Astoria (loading) July 11 13 CR Estuary (SIS) July 14 17 Astoria Rainier Portland Rainier Astoria (RS) July 18 19 CR Estuary South Channel (ETM) July 20 22 CR Estuary North and South Channel (ETM) July 23 transit

G. Operational Details: July 7: Barnes travel day July 8: Barnes travel day July 9 9:00: Loading in Astoria loading and setup (all participants) install ADCP (Wilkin) install incubator (Needoba/ Postel) test CTD, ADCP, pump (Needoba/Wilkin/Postel) July 10: Point Adams transects 6:00 transit to Point Adams (6 miles) (46 12 33 N, 123 56 57 W) 7:00 primary production (PP) collection 8:00 4:00 ADCP survey, CTD and water sample surveys. South channel near Point Adams Collect water for salinity experiments on the 15th 4:30 6:00 transit back to Astoria (6 miles) July 11: Salt Intrusion Survey 1 (SIS 1) 6:00 transit to region of predicted salt intrusion (SI) (Up to 10 miles) (46 15 27 N, 123 39 24 W) 8:00 4:00 SI survey 4:00 6:00 transit back to Astoria (Up to 10 miles) July 12: SIS 2 6:00 transit to SI (Up to 10 miles)( (46 15 27 N, 123 39 24 W) 7:00 PP collection 8:00 4:00 SI survey 4:00 6:00 transit back to Astoria (Up to 10 miles) July 13: SIS 3 6:00 transit to upper extent of SI (Up to 10 miles) ( (46 15 27 N, 123 39 24 W) 8:00 4:00 SI survey 4:00 6:00 transit back to Astoria (Up to 10 miles) July 14: River survey (RS) 6:00 leave Astoria 8:00 4:00 River survey 4:00 6:00 Arrive in Rainier (50 miles total trip) (46 05 40 N, 122 56 25 W) July 15: RS 2 6:00 leave Rainier 7:00 Salinity experiment set up 8:00 4:00 River survey Columbia east of Portland (45 miles) (46 36 05 N 122 35 18 W) 4:00 Arrive in Portland/Vancouver (45 37 20 N 122 41 07 W) July 16: Crew change, RS 3 12:00 Sample Willamette, Columbia, back to Rainier (45 miles) (46 05 40 N, 122 56 25 W) July 17: RS 4 6:00 leave Rainier 8:00 4:00 Survey back to Astoria (50 miles)

July 18 : ETM 1 6:00 travel to South Channel ETM (7 miles) (46 12 33 N, 123 56 57 W) 8:00 4:00 survey/chase ETM July 19 ETM 2 6:00 travel to South Channel ETM (7 miles) (46 12 33 N, 123 56 57 W) 8:00 4:00 survey/chase ETM July 20 ETM 3 6:00 transit to North channel near Saturn 01 (14 miles)(46 14 08 N 123 53 26 W) 9:00 4:00 survey/chase ETM 4:00 6:00 transit to Astoria/Warrenton July 21 ETM 4 6:00 transit to North channel near Saturn 01 (14 miles)(46 14 08 N 123 53 26 W) 7:00 PP collection 9:00 4:00 survey/chase ETM near Saturn 01 4:00 6:00 transit to Astoria/Warrenton July 22 ETM 5 6:00 transit to South Channel ETM (7 miles) (46 12 33 N, 123 56 57 W) 7:00 12:00 ETM Survey 1:00 4:00 Unload ship, science team finished July 23 Barnes travel day July 24 Barnes travel day H. Overnight locations: July 7 transit July 8 transit July 9 Astoria (MERTZ or Maritime Museum) July 10 Astoria July 11 Astoria July 12 Astoria July 13 Astoria July 14 Rainier July 15 Portland July 16 Rainier July 17 Astoria July 18 Astoria July 19 Astoria July 20 Astoria July 21 Astoria July 22 Astoria July 23 transit July 24 transit

I. Study descriptions: Point Adams survey (July 10) Purpose of this one day survey is to conduct cross channel surveys of the South Channel of the Columbia River Estuary near Point Adams. The ADCP will collect velocity data. The CTD/pump system will be used to collect water column profiles of water properties at specified regions along the transect. The transect data will be used to calibrate and characterize the Saturn 03 suite of instruments located near Point Adams. Salt Intrusion Survey (SIS) (July 11 13) Purpose of the SIS to identify and characterize the limits of Salt intrusion associated with the estuarine salt wedge. Location and sample collection will be determined using CMOP modeling capabilities. ADCP and CTD/pump samples will be collected at high resolution to characterize the physical, chemical, and biological properties of the salt wedge. Once identified, the salt wedge will be characterized through CTD measurements and water sample collection River Survey (RS) (July 14 17) Purpose of the RS is to collect biological and chemical data along the lower Columbia River between Astoria and Portland. CTD/pump samples will be collected each hour during transit. Estuarine Turbidity maximum (ETM) (July 18 22) Purpose of the ETM survey is to collect physical, biological and chemical data to characterize the ETM in both the South and North Channel. The North Channel samples will also be used to characterize the Saturn 01 suite of instruments located on Pier 11 of the AM bridge. Sample frequency of ETM is defined by microbiology requirements. If first day is successful, the second day in each channel will be devoted to cross channel ADCP and CTD surveys. J. Cast details and maximum water samples July 7 transit July 8 transit July 9 loading/ practice casts July 10 6 ADCP/CTD across channel transects (3 casts per transect), water samples from 3 transects surface, middle, bottom (3 x 3 x 3 = 27) July 11 CTD every hour, WS from 3 points across salt wedge at 3 depths, morning and afternoon (18) July 12 CTD every hour, WS from 3 points across salt wedge at 3 depths, morning and afternoon (18) July 13 CTD every hour, WS from 3 points across salt wedge at 3 depths, morning and afternoon (18) July 14 CTD and water samples every hour from 2 depths (8 x 2 = 16) July 15 CTD and water samples every hour from 2 depths (8 x 2 = 16) July 16 CTD and water samples every hour from 2 depths (4 x 2 = 8) July 17 CTD and water samples every hour from 2 depths (8 x 2 = 16) July 18 CTD 30 min water sample at low tide, peak etm, post etm 3 depths (9) more if possible (9) July 19 6 ADCP/CTD across channel transects (3 casts per transect), water samples from 3 transects surface, middle, bottom (3 x 3 x 3 = 27) July 20 CTD 30 min water sample at low tide, peak etm, post etm 3 depths (9) more if possible (9) July 21 6 ADCP/CTD across channel transects (3 casts per transect), water samples from 3 transects surface, middle, bottom (3 x 3 x 3 = 27) July 22 CTD every hour, WS from 3 points across salt wedge at 3 depths (9) July 23 transit July 24 transit Total water samples = 218

K. Sample description CTD Salinity, temperature, depth, light transmission, fluorescence, oxygen, variable fluorescence ADCP Current velocity WS Water sample: Macro Nutrients, chl a, DOC, TDN, TDP, POC, PON, SPM, RNA, DNA, HPLC, CDOM, FISH Bacterial productivity (BP) 3 H leucine incorporation 1 hr incubations Primary productivity (PP) 13 CO 2, 15 NO 3 and 15 NH 4 + incorporation 24 hr dawn dawn incubations L. Approximate Area of transit and operation (White line with small red dots look closely!) July 10 Point Adams

July 11 13 Salt intrusion region

July 14 Astoria to Rainier July 15 Rainier to Portland

July 16 Portland to Rainier July 17 Rainier to Astoria

July 19 22 North and South Channel ETM