LONGIDORUS PIUS SP. N. (NEMATODA: LONGIDORIDAE) FROM MACEDONIA

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1 Nematol t~7edit (2001) Institute of Biology, Faculty of Science, Universi& of Novi Sad, Novi Sad, Yz/goslavia Istituto di fvematologia Agraria, CNR Bari, Italy LONGIDORUS PIUS SP N (NEMATODA: LONGIDORIDAE) FROM MACEDONIA Summary Longido~*z~s pfus sp n,, a monosexual species found in the rhizosphere of Ca~pinus orientalis at Sveti Jovari Bigorski Monastery on the slope of Mount Bistra in Macedonia is described The species is characterized by its medium size (L=jO68 mm) and thick body (a=%68); anteriad abmptly tapering and laterally rounded lip region, continuous with the rest of the body; pocketsliaped amphids which are not bilobed at the base; long odontostyle ( pm); and short, rounded tail L pius sp n has four juvenile developmental stages; the first stage with digitate tail L pzus sp n reseriibles L picenus and L neuesi A Longido~pus species, unknown to the best of our knowledge, was extracted from soil samples collected in Apri from the rhizosphere of Carpinus olpientalis at Sveti Jovan Bigorski Monastery on the slope of Mount Bistra (Macedonia) The population is described here as L pius sp n Materia1 and methods Nematodes were extracted by Cobb's wet sieving technique Specimens were killed by hot FP 41 and processed and mounted on perinanent slides in dehydrated glycerin Measurements were made with an eyepiece scale, except body length, which was determined with the aid of a drawing tube and map measurer Description LONGIDORUS PIUS sp n (Table I; Figs 14) Female habitus more or less ventrally curved with the curvature inore pronounced posteriad in some specimens Body of medium size, robust and cylindrical, tapering 17e1y gradually towards anterior Cuticle 56 pin thick at midbody, smooth in appearance, sometimes with very fine transverse striations which are always visible in the tail region Lip region continuous with the rest of the body, tapering abruptly anteriad and laterally rounded Labial papillae prominent Ainphidial pouches large, pocketsliaped without distinct basal lobes Odontostyle, odontophore and guiding ring typical of the genus A mucro is visible at the base of the odontophore or just behind it Oesophagus dorylaimoid, with the basal bulb occupying 1/4 1/5 of the total oesophagus length and measuring pm long and 3336 pm wide; it contains three glandular nuclei Oesophagointestinal valve cylindrical Vulva a tranverse slit, at inidbody; vagina extending to 4060% of corresponding body diameter; thick walled pars proximalis vaginae 2131 pm long Gonads amphidelphic, of approximately the same length and structure; ovaries reflexed Prerectum pm long (n= 101, rectum as long as 0609 body diameter at anus Tail bluntly conoid,

2 TABLE I Morphonzetrics oj'longidonis pius sp 72 j'rorn Macedo7zia Holotype Pardtypes n Q 25 Q J2 13 J L (mm) k k k (50682) (12169) (l 58267) (253362) (38350) a k k20 569k364 (584684) (40495) (416507) (472547) (509618) b k06 85k061 (84128) (3954) (4774) (6486) (7994) C k k64 974k1105 ( ) (263349) (49718) (699926) ( ) C' k k k005 v (057074) (16723) (079118) (068086) (064079) k127 (480545) Odontostyle pm k k k a372 ( ) (675737) (794869) ( ) ( ) Odontophore pm k k k221 (738875) (42550) (488638) (575713) (725788) Total stylet prn k k k573 ( ) ( ) ( ) ( ) ( ) Replacernent 839k k k k330 odontostyle pm (8089) ( ) ( ) ( ) Ora1 aperture k k k to guiding ring pm (350419) (194219) (231275) (275331) (331363) Tail pm k (393503) (403525) (321418) (343453) (40489) J (hyaline portion k _+121 ii of tail) pm (159197) (15197) (84125) (10138) (113163) Body diam at lip k072 98k k k053 region pm (208234) (9410) (117138) (138175) ( Body diam at k k a420 guiding ring pm (397472) (166184) (204247) (281322) (25640) Body diam at base k k k k of oesophagus pm (716854) (272338) (338513) (463625) (642725) Body diam at mid k k k496 body or vulva pm (80105) (272375) (33860) (463729) (713853) Body diain rt k k at anus pm (61375) (213294) (30466) (438604) (55653) Body diam at k k k k261 beginning of J pm (413513) (109138) (188313) (288388) (35438)

3 Fig 1 Longicio~7~spius sp n,: A, female anterior region; BC female tail; DG, tail of J1, J2, J3 and 14 stage, respectively rounded to almost hemispherical, bearing two Type baliitat arzd locality Rhizosphere of or three caudal pores on each side Cnrpi~zus orieiztalis Mill at Sveti Jovan Bigorski Male not found Monastery (UTM: DM6O) on the slope of Mount Juveniles separate into four groups (Fig 4) Bistra, Macedonia The first juveniles stage with digitate tail Type material Holotype, 10 female and 30

4 Fig 2 Phommicrographs of L pnrs sp n: A, femaie amior regiwi; RD, fede amphidid p&; E, ju of the: don e with he oclontophorie, F, dva region; G, ammior uww; HI, fede tail 210

5 Fig 3 Photomicrographs of juvede stages of L piw sp n AD, &or mil ofji, Jz, J3 and J4 1~ regian of JI, J2, J3 d J4 smge, respectivel~ EH,

6 C, juvenile paratypes in the collection of the Istituto di Nematologia Agraria del Consiglio Nazionale delle Ricerche, Bari, Italy; 11 female and 33 juvenile paratypes, Institute of Biology, Novi Sad, Serbia; two female paratypes, CABI Bioscience Centre, Egham, United Kingdom; two female paratypes, Plant Nematology Laboratory Collection, United States Department of Agriculture, Beltsville, Maryland, USA Diagnosis and relationships Longidorus pius sp n is a monosexual species of medium size (~=5068 mm) and thick body (a = 5868), with laterally rounded lip region, continuous with the rest of the body, pocketshaped amphids which are not bilobed at the base, long odontostyle ( pm), and short, rounded tail; juvenile developmental stages separate into four groups; digitate tail in the first stage juvenile The code for identifying the new species when using the polytomous key of Chen et al (1997) and ~oof and Chen (1999) is: A 56, B 45, C34,Dl,El,F23,Gl,Hl,Il Longidoms pius sp n is similar to L pice~zus Roca, Lamberti et Agostinelli, 1985 and L nevesi Macara, 1986 However, it differs from both in the amphids not being bilobed at the base Further, L pius differs from L picenus in its lower value of a (8090 in L picenus) and C ( in L picenus) ratios and from L neuesi in its shorter body (8 mrn in L neuesi), lower a ratio value (72102 in L neuesz] and less rounded lip region (almost hemispberical and pointed in L neuesi) _ Odontostyle Replacement odontostyle n = 89 J2 o 6&OB0 % J3 (2 (:ti) 1 (2 L* ai e* J1 8 0 J ( &A*& Females J4 [bo O (3 pe 't 0 I I I l l I l 1 I I l Body length (mm) Fig 4 Scatter diagram separatiiig juveniles and females of L pius sp n

7 Acknowledeement a We thank Ivan Dulic' laimoidea) Fz~ndamental and Applied Nematology, 20: 1528 'C', Naftagas> Novi giving Loor p, A, A and CnrN Q,, 1999, A revised polytomous t0 an Olyinpus BX50 photomicroscope kev for the identification of snecies of the eenus Lonaido'rus Micoletzky, 1922 (~ematoda: ~or&imoidei] Supplement 1 Nematology, 1: 5559 MACARA M, 1986 TWO new species of Longidorus (Ne Literature cited matoda: Longidoridae) associateci with forest plants in Portugal Nematologica, 32 (1985): CHEN Q, HOOPER D J, LOOF P A A and Xu J, 1997 A re ROCA F, LAMBERTI F and AGOSTINELLI A,, 1985 Three new vised polytomous key for identification of the genus species of Longidonrs (Nematoda, Dorylaimida) from Longidorus Micoletzky, 1922 (Nematoda: Dory Italy Nematologia Meditewanea, 12 (1984): Accepted for publication on 18 June 2001

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