THINGS YOU FORGOT OR DIDN'T KNOW ABOUT EMPOWER

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1 Le Jeudi 02 février 2017 Maison de l Amérique Latine Paris 7ème Présenté par Alain Barreau THINGS YOU FORGOT OR DIDN'T KNOW ABOUT EMPOWER 2016 Waters Corporation 1

2 Things you forgot or didn t know about Empower Empower 3 is powerful with many features available In this presentation we show >20 topics that You might have forgotten, you might not know, or are completely new. We will try to show all the slides, but only have 1 hour so we ll see how far we get! All the options are available in Empower 3 FR3, our most recent release Waters Corporation 2

3 Topics listed View Filters [ 4] View As Functionality [ 13] Result Audit Viewer [ 16] Global Project view [ 25] Custom Field to check Manual Integration [ 32] JP, EP, USP Signal to Noise [ 38] Shifting peaks (relative) [ 43] Who is using what System now (Mobile) [ 55] Protect Systems and Nodes from being deleted [ 65] Protect Parent Projects from being acquired in [ 69] Copy Settings to Sub-Projects [ 72] Add Peaks and Integration events during Review [ 74] ID s in project view [ 78] Copy and Paste [ 81] Export to JCAMP [ 86] Compare Calibration Curves [ 88] Empower Analytics [ 90] Current User/System licence status [ 94] Walk-Up Interface [ 96] Mass Analysis Window [ 102] Location Field [ 107] MS Peak Tracking [ 109] ICH Impurity [ 114] Instrument Control [ 119] 2016 Waters Corporation 3

4 View Filters 2016 Waters Corporation 4

5 View Filter Just in Time Any table in Empower can apply a filter Just in Time Code for Just in Time is: =# Example: Fill information: Now the projects with Default in the name are shown 2016 Waters Corporation 5

6 View Filter Just in Time A maximum of three =# options is possible Fill the Just in Time filter This gives an overview of the requested projects 2016 Waters Corporation 6

7 View Filter FAT Settings of Projects Click Edit View on the Project View, and add the following 8 columns to the view. Save the View Filter and Apply Now for each of the projects the FAT settings are shown 2016 Waters Corporation 7

8 View Filter See latest Result Set To only see the latest Result Set(s) belonging to corresponding Sample Set(s), edit the view filter In the column Result Set ID create a Group By Condition Enter the following Information The information is filled, then save and apply the filter Latest Result Set(s) of the Sample Set(s) are shown 2016 Waters Corporation 8

9 View Filter Last User Login To see the last user login after a certain date. Go to System Audit Trail (1221 records) Click Edit View to start creating the Filter Use the View Filter Wizard 2016 Waters Corporation 9

10 View Filter Last User Login Wizard is started Select the Displayed Columns: Click Next > until Save View Filter now: Enter Name and Click Finish 2016 Waters Corporation 10

11 View Filter Last User Login Select in the 1st row Sequence and start the Group By function Fill the Group By Condition, Click OK Now fill the rest of the filter, save the filter 2016 Waters Corporation 11

12 View Filter Last User Login Upon applying the filter, the change date needs to be entered Any successful login after this date is displayed (8 records only) 2016 Waters Corporation 12

13 View As Functionality 2016 Waters Corporation 13

14 View As functionality To see the relation between all data in an Empower project, the view as functionality is powerful It works from all tables including view as Audit Records 2016 Waters Corporation 14

15 View As functionality There is an extra option available When you want to go back to the previous view simply select View as previous View as next That way you can easily navigate back and forth 2016 Waters Corporation 15

16 Result Audit Viewer 2016 Waters Corporation 16

17 Result Audit Viewer To review data, the Result Audit Viewer can be used Open a Result Set into review Browse to the icons on the top right Alter Sample Print Preview Run Sample Result Audit Viewer Click Result Audit Viewer icon and the viewer is started All information from the Result Set can be viewed using one page 2016 Waters Corporation 17

18 Result History 2016 Waters Corporation 18

19 Result Differences 2016 Waters Corporation 19

20 Results can then be compared Result ID 1453 and 1463 can be taken into review Using overlay the slight difference in the integration can be shown 2016 Waters Corporation 20

21 Result Processing Method 2016 Waters Corporation 21

22 Result Sample Set Method 2016 Waters Corporation 22

23 Result Instrument Method 2016 Waters Corporation 23

24 Result Method Set 2016 Waters Corporation 24

25 Global Project View 2016 Waters Corporation 25

26 Global Project View In the project view, select 2 (or more) projects Right Mouse Click, Open The projects are opened in a Global Project View 2016 Waters Corporation 26

27 Global Project View Simply activate or deactivate additional projects by clicking the available projects button The following view is shown Select an additional project and Click Activate Project is added to the Global Project view 2016 Waters Corporation 27

28 Global Project View - Search The view filters also work across multiple projects In this example a filter for Sample Name Sample 6 is applied Two different projects and the same Sample Name is shown With the same sample name selected in the Injection, simply select View As Results Results of the corresponding Injections in two projects are displayed 2016 Waters Corporation 28

29 Global Project View - Compare With the same sample name selected in the previous slide we can simply overlay those results Select the two results, right mouse click Compare Two results will be shown Apply the following settings to display Properties, Overlay, Overlay in Single Plot Properties, Overlay, All Chroms in Overlay 2016 Waters Corporation 29

30 Global Project View - Compare Both Results (including the integration) are shown in the overlay plot 2016 Waters Corporation 30

31 Global Project View Audit Trail Within the Global Project View it is also possible to see both the Project and the System Audit Trail In the Global Projects tree, select Audit Trails 2016 Waters Corporation 31

32 Global Project View Audit Trail Within the Global Project View it is also possible to see both the Project and the System Audit Trail In the Global Projects tree, select Audit Trails Now the Audit Trails are shown for System and Project We can use view filter to search specific actions System Audit Trail Projects Audit Trail 2016 Waters Corporation 32

33 Custom Field to check for Manual Integration 2016 Waters Corporation 33

34 Custom Field to check for Manual Integration To easily check for Manual Adjustment of Integration, one can create a Custom Field that checks for these changes using the Integration Type of the peak See proposed formula 2016 Waters Corporation 34

35 Custom Field to check for Manual Integration Remember: In case of regulated / controlled work environment, the Custom Fields as shown in this example, do need structural testing and validation prior to its usage The proposed formula: EQ(EQ(Int Type,"BB")+EQ(Int Type,"BV")+EQ(Int Type,"VB") +EQ(Int Type,"VV")+EQ(Int Type,"EE")+EQ(Int Type,"VE") +EQ(Int Type,"EV")+EQ(Int Type,"TV")+EQ(Int Type,"VT") +EQ(Int Type,"TT")+EQ(Int Type,"BS")+EQ(Int Type,"SB") +EQ(Int Type,"VR")+EQ(Int Type,"RV")+EQ(Int Type,"BG") +EQ(Int Type,"GB")+EQ(Int Type,"VX")+EQ(Int Type,"XV") +EQ(Int Type,"Group"),0) 2016 Waters Corporation 35

36 Custom Field to check for Manual Integration Result in Review 2016 Waters Corporation 36

37 Custom Field to check for Manual Integration Result in Review Slight adjustment would immediate flag the peak for change in Integration Type, or addition of peaks 2016 Waters Corporation 37

38 Custom Field to check for Manual Integration This can be taken a step further by creating a Result Type Custom Field using the previous Calculation as a Use As Value See proposed formula EQ(SUM(IntVal),0) 2016 Waters Corporation 38

39 Custom Field to check for Manual Integration These Custom Fields can also be brought into Preview 2016 Waters Corporation 39

40 JP, EP, USP Signal to Noise 2016 Waters Corporation 40

41 JP, EP and USP Signal to Noise S/N calculations can be done without the use of a Custom Field It is calculated as follows S/N 2H h Where H is the height of the peak measured from the peak apex to a baseline extrapolated Where h is the difference between the largest and smallest noise values observed over a distance of x times the width at the half-height of the peak if possible, situated equally around the peak of interest after the injection of a blank Processing method, Suitability Tab * Depends on the used Pharmacopoeia 2016 Waters Corporation 41

42 JP, EP and USP Signal to Noise Allows determination of noise within a blank injection centered on the peak region Allows user to specify a noise segment based on peak width at half height: USP Half Height Multiplier for Noise Region EP Half Height Multiplier for Noise Region JP Half Height Multiplier for Noise Region Using more blanks calculates multiple noise values for each blank injection, then uses the average of the noise value 2016 Waters Corporation 42

43 JP, EP and USP Signal to Noise Half Height Multiplier for EP s/n region = 5 That gives the following regions for the calculation 2016 Waters Corporation 43

44 JP, EP and USP Signal to Noise In the Results tab an extra column is available to see what the Channel IDs were used for Blank in the S/N calculation even if more than one blank is used 2016 Waters Corporation 44

45 Shifting peaks (Relative) 2016 Waters Corporation 45

46 Shifting peaks (Relative) If you have a chromatogram with many peaks, the alignment might be a lot of work Changing mobile phase Changing method settings Empower can help to overcome this 2016 Waters Corporation 46

47 Review window: Method Set Option 2016 Waters Corporation 47

48 Review window: Zoom settings 2016 Waters Corporation 48

49 Processing Method Layout As an example the last 5 peaks will be used 2016 Waters Corporation 49

50 Processing Method Layout Manually identify peaks 2016 Waters Corporation 50

51 Manually assign peak 1 to the peak at 27.2 min Peak 2, Peak 3, Peak 4 and Peak 5 are not effected by the manual assignment of Peak Waters Corporation 51

52 Setup processing method for critical peak (groups) If peak 1 is modified, you should verify peak 2 too! 2016 Waters Corporation 52

53 Must function in combination with RT Reference Peak 2 is effected by the manual assignment of Peak 1. Peak name disappears and as peak 2 is a must peak it flags red if the peak is not present / not assigned Waters Corporation 53

54 Relative Retention Time (RRT) RRT = RT of the peak / RT of the RT Reference 2016 Waters Corporation 54

55 Manually assign peaks Now Peak 2 and Peak 3 are automatically assigned to the correct peaks after the the manual assigment of Peak Waters Corporation 55

56 Conclusion Avoid repetitive action: by linking the components Extra check in review if all peaks have been identified by the use of the must function in the processing method 2016 Waters Corporation 56

57 Who is using what system now? Mobile? 2016 Waters Corporation 57

58 Who is using what system now? To check the licenses in use, View the System Monitor Of the two systems available, not one is currently in use In case no systems are shown, go to View, Options Select Show All Objects 2016 Waters Corporation 58

59 Or one can go use Empower Mobile 2016 Waters Corporation 59

60 Login with normal Empower account 2016 Waters Corporation 60

61 Show your Favorite Systems 2016 Waters Corporation 61

62 Show all Systems 2016 Waters Corporation 62

63 Track Running Injections 2016 Waters Corporation 63

64 And also Administrative Tasks 2016 Waters Corporation 64

65 Check User Account 2016 Waters Corporation 65

66 Change User Password 2016 Waters Corporation 66

67 Protect Systems and Nodes from being deleted 2016 Waters Corporation 67

68 Protect Systems and Nodes from being deleted If you do not want your Systems and Nodes being deleted (e.g. by mistake) follow these steps Open the properties of the Node, select Access Tab Check the Group(s) you Allow Access to 2016 Waters Corporation 68

69 Protect Systems and Nodes from being deleted Then change the allowed access to Owner Only Click OK, login as other user (= NOT Administrator) 2016 Waters Corporation 69

70 Protect Systems and Nodes from being deleted The node can be seen and the Properties cannot be changed! Reboot of the LAC/E is still allowed and possible Selecting and using the corresponding LC system is possible deleting the LC system is not 2016 Waters Corporation 70

71 Protect Parent Projects from being acquired in 2016 Waters Corporation 71

72 Protect Parent Projects from being acquired in By removing group access from the Parent Projects, these projects can still be seen, but access is restricted Colour of the folder is greyish/yellow No Access (only Admin) Used for Acquisition No Access (only Admin) Used for Acquisition No Access (only Admin) 2016 Waters Corporation 72

73 Protect Parent Projects from being acquired in By removing group access from the Parent Projects, these projects can still be seen, but access is restricted Colour of the other folders is bright-yellow No Access (only Admin) Used for Acquisition No Access (only Admin) Used for Acquisition No Access (only Admin) 2016 Waters Corporation 73

74 Copy settings from Parent Projects to Sub-Projects 2016 Waters Corporation 74

75 Copy settings from Parent Projects to Sub-Projects Changes or settings in a Parent can easily be copied to its corresponding Sub Projects Press Copy to Sub-Projects Accessible on General, Access and Processing Copy to Sub-Projects works on the selected settings before Clicking OK one can change the settings for the Parent to be different 2016 Waters Corporation 75

76 Add Component Names and Integration Events during Review 2016 Waters Corporation 76

77 Add Component Names during review At first view no peaks are assigned Right mouse click on the peak, select Add/Edit Component Name Enter the component name Click OK 2016 Waters Corporation 77

78 Add Integration Event during review Component name is added to the method and applied to the chromatogram Right mouse click in the chromatogram, select Add Integration Event Add the Integration Event Click OK 2016 Waters Corporation 78

79 Add Integration Event during review The integration event is inserted and applied to the chromatogram 2016 Waters Corporation 79

80 ID s in Project View 2016 Waters Corporation 80

81 ID s in Project View Normal Project view with Default View Filter, would not show the ID s One could create view filter to always show the ID s But in Empower 3 there is a possibility to Persist ID columns in Project That way the ID s will always be visible for all Project Tabs 2016 Waters Corporation 81

82 ID s in Project View In Sample Sets In Result Sets And also in Injections, Channels, Methods, Results, Peaks, Fractions, Sign Offs, and Curves 2016 Waters Corporation 82

83 Copy and Paste 2016 Waters Corporation 83

84 Copy and Paste From Review / Preview / any Tables From Review, select the chromatogram Right Mouse Click, Copy Paste into Word Excel 2016 Waters Corporation 84

85 Copy and Paste From Preview, select the chromatogram Right Mouse Click, Copy Paste in Word Paste in Excel as picture 2016 Waters Corporation 85

86 Copy and Paste From Preview, select area to copy to the clipboard Select the Area, on release it is copied to clipboard Paste in Word Paste in Excel 2016 Waters Corporation 86

87 Copy and Paste On a Table, select the information needed From the menu select File, Copy Table Paste in Excel 2016 Waters Corporation 87

88 Export to JCAMP 2016 Waters Corporation 88

89 Export to JCAMP Directly export data* to JCAMP without creation of an export method Select the data* In the menu select Database, Export to JCAMP Exported data can be opened using an external viewer * Sample Sets, Samples, Channels 2016 Waters Corporation 89

90 Comparing Calibration Curves 2016 Waters Corporation 90

91 Comparing Calibration Curves Calibration Curves can easily be compared Select the Curves of Interest Right Mouse click, and select compare Easily the difference between the selected curves can be seen 2016 Waters Corporation 91

92 Empower Analytics 2016 Waters Corporation 92

93 Empower Analytics Empower Analytics offers business owners, system managers, and laboratory managers insight into business processes, work flows, and laboratory operations Empower Analytics consists of various dashboard reports that present statistical data scanned from the Empower database. The pre-defined statistical reports provide both summary and detailed information that can assist you in deciding where and how to improve laboratory efficiency In Configure the System From the menu select Tools Select Empower Analytics 2016 Waters Corporation 93

94 Empower Analytics The first step in your System Overview and Usage 2016 Waters Corporation 94

95 Empower Analytics Dashboard - Insight in your manual results 2016 Waters Corporation 95

96 2016 Waters Corporation 96

97 Current User/System licence status 2016 Waters Corporation 97

98 Current User/System licence status To check the licenses in use, View the System Monitor The active licenses are visible using the Waters License Wizard Click, Show Licenses All the activated licenses are listed 2016 Waters Corporation 98

99 Walk-Up Interface 2016 Waters Corporation 99

100 Walk-Up Interface This interface is designed for high-throughput laboratories where users walk up to a computer system and run samples using predetermined methods. Simplified Interface for easy access and acquisition of Samples Log in, and select the project and the system Waters Corporation 100

101 Walk-Up Interface Fill the interface. Method, System, Sample Names, Method Set, Number of Samples, Start Position, Injection Volume, Run Time give the Sequence Name Press Run Sample(s) Place the Samples in the System Press OK Waters Corporation 101

102 Walk-Up Running The Samples will be injected, system status can be shown Waters Corporation 102

103 Walk-Up Method Set With the Run and Process option set, and when the Method Set contains a Report Method, the injection is: Processed with given Processing Method Reported with given Reporting Method Printed, if printer is selected! With the properties in the Walk-Up, the interface can be set to meet the needs of the groups using the Walk-Up interface The slides with the properties are given in the next page 2016 Waters Corporation 103

104 Walk-Up Properties There are 6 additional properties available in Walk-Up Interface 2016 Waters Corporation 104

105 Mass Analysis Window 2016 Waters Corporation 105

106 Qualitative MS: Mass Analysis A new Mass Analysis window is added to Review which will allow users to view and analyze the PDA and MS spectral and chromatographic data from a single result The window is displayed by clicking on the Mass Analysis button in the Window menu. Window is empty when the current result does not contain PDA or MS data Start review from the injection or the PDA channel 2016 Waters Corporation 106

107 Mass Analysis Window: Start from review: Step 1 PDA 2016 Waters Corporation 107

108 Mass Analysis Window: Setup in properties (right click) Access to properties: modification of the mass analysis view Fast review: Access to icons/buttons: modification of the mass analysis view 2016 Waters Corporation 108

109 Mass Analysis Window: New window: Step 2 PDA & MS Spectra of each Integrated Peak PDA Chromatogram, Extracted Wavelength MS XIC for all Integrated Peaks Automatic Extraction of XIC Chromatograms from most intense m/z Peak 2016 Waters Corporation 109

110 Location Field 2016 Waters Corporation 110

111 Location Field With Empower 3 FR3 the location field has been introduced List of Locations can be maintained in the Default Strings Then it can be applied to Users Nodes Systems 2016 Waters Corporation 111

112 MS Peak Tracking 2016 Waters Corporation 112

113 MS Peak Tracking Available in Empower 3 FR3 Processing method enhanced MS Peak Tracking option Tracking options None Apex Combined Number of decimal places 2016 Waters Corporation 113

114 MS Peak Tracking New fields Assigned Mass Notes Automatically assigns mass Using Base Peak (m/z) For peaks of the same mass but different retention times Option to manually assign mass to override auto assignment Add notes for manually assigned mass 2016 Waters Corporation 114

115 Properties: Peak Labels 2016 Waters Corporation 115

116 Report: Component Summary Table (for unknown peaks!) m/z Retention Time Column Type 2016 Waters Corporation 116

117 ICH Impurity Deviating Thresholds for Specified Impurities 2016 Waters Corporation 117

118 Deviating Thresholds for Specified Impurities 2016 Waters Corporation 118

119 Summary Slide 2016 Waters Corporation 119

120 Summary Slide 2016 Waters Corporation 120

121 Automatically calculate total impurities 2016 Waters Corporation 121

122 Instrument Control 2016 Waters Corporation 122

123 Instrument Control Empower 3 FR3 has a possibility of controlling 472 Instrument Modules from 16 Vendors (and growing) 2016 Waters Corporation 123

124 Instrument Control 2016 Waters Corporation 124

125 Instrument Control 2016 Waters Corporation 125

126 Instrument Control 2016 Waters Corporation 126

127 Instrument Control 2016 Waters Corporation 127

128 Instrument Control 2016 Waters Corporation 128

129 Instrument Control 2016 Waters Corporation 129

130 Instrument Control 2016 Waters Corporation 130

131 Instrument Control 2016 Waters Corporation 131

132 Instrument Control 2016 Waters Corporation 132

133 Instrument Control 2016 Waters Corporation 133

134 Instrument Control 2016 Waters Corporation 134

135 Instrument Control 2016 Waters Corporation 135

136 Instrument Control 2016 Waters Corporation 136

137 Instrument Control 2016 Waters Corporation 137

138 Instrument Control 2016 Waters Corporation 138

139 Instrument Control 2016 Waters Corporation 139

140 Instrument Control 2016 Waters Corporation 140

141 Instrument Control 2016 Waters Corporation 141

142 2017 Waters Corporation COMPANY CONFIDENTIAL 142 Solution Overview Import weights from balances directly into Empower Starts from inside Empower, no additional login required. Interacts with an Empower Sample Set Method Map weights to sample and component fields. Import weights to existing and running sample set methods Keeps a complete audit trail of all weights acquired for FAT projects

143 Mapping weights to a sample set method 2017 Waters Corporation COMPANY CONFIDENTIAL 143

144 2017 Waters Corporation COMPANY CONFIDENTIAL 144 Basic Solution Architecture Ethernet Conversion (if required by balance) Direct Cable Connection Client PC SDS Empower Empower Toolkit Sample Weight Importer

145 2017 Waters Corporation COMPANY CONFIDENTIAL 145 Advanced Solution Architecture Empower Network Tibbo Device to connecting a balance to network Ethernet Conversion (if required by balance)

146

147 2017 Waters Corporation COMPANY CONFIDENTIAL 147 Rencontrer nos experts Salle actuelle Salon Miranda (RdC près du vestiaire) Logiciels Instrumentations Consommables Service Solutions informatiques Gammes Colonnes Contrats Applications Préparation d échantillons Flacons SAV Formation Aurélie Chambon Taraneh Kargar-Grisel Gilles Jaouen Reda Zarrou Alain Barreau Isabelle Jarecki Myriam Harmouche Sébastien Lahalle Damien Martin Bruno Contrastin

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