HERD project: STUDY OF THE MICROBIOLOGICAL FLORA OF MILK AND DAIRY PRODUCTS IN KOSOVO

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1 HERD project: STUDY OF THE MICROBIOLOGICAL FLORA OF MILK AND DAIRY PRODUCTS IN KOSOVO The microbial flora (quality) of (i) milk and (ii)dairy products in Kosovo: With emphasis on the dairy lactic acid bacteria and the two pathogens Listeria monocytogenes and Staphylococcus aureus

2 Research Education Information Farmers and Company

3 Institutions and People Involved University of Pristina: Ibrahim Mehmeti, Post. Doc. Skender Muji, Professor Hysen Bytyqi, Professor 2 Master student (right now) Selami Salihu (Master) Ibadete Krasniqi (Master) Imer Haziri (PhD student) 3 Bachelor student (Diploma for Bachelor) Norwegian University of Life Sciences: Ingolf F. Nes, Professor Dzung Bao Diep, Professor Technical Support

4 EDUCATION

5 Master student Ibadete Krasniqi (UP- Pristina Kosovo) Selami Salihu (Agriculture University - Tirana Albania)

6 Bachelor student 1. Remzi Jetullahu 1. Valdet Thaqi 2. Idriz Lalloshi

7 Work Training at LMG-UMB 1.Imer Haziri (PhD student) 1.Ibadete Krasniqi Master student

8 Laboratory Manuel Microbiology and food microbiology Agriculture Faculty and Veterinary used for students, farmers and company

9 RESEARCH

10 RESULTS I. Isolation and characterization of bacteria in milk obtained from producers

11 Sampling of milk for bacterial analysis Communities: 10 Farms: 237 Number of samples: 711 (3 tomes from each farm) In December-February (237 samples) In March- April (237 samples) In May-June (237 samples) Bacterial isolates: 5000

12 Samples collection Sharri Rahovec Skenderaj Lipjanë Podujeva Istogu Skenderaj Istogu Rahoveci Gjakova Theranda Ferizaji Sharri Sharri

13 Total Viable Count 35 TVC /ml 30 28% complyies with the regulation , ,99 6,33 9,42 7,6 6,91 5,48 5,88 5,49 6,47 5,14 0 < < < < < < < < < < <

14 Analyses 5000 different strains are collected 3000 Analyses in Norway 888 are analyzed for biochemical testes: Growth at different ph (6.0 and 9.2) Growth at different NaCl comcentration (2% and 6.4%) Growth in two different mediums: MRS and GM17 Gas production(homo-fermentative and hetero-fermentative) 888 LAB for bacteriocin production Multi - Locus Sequencing -Typing (MLST)?

15 Classification Present status of 3000 isolates: Staphylococcus aureus: 235 isolates Listeria spp. 112 isolates Lactic acid bacteria: 2653

16 Meeting and visits in farms Farms Higher number (more contaminations) Between higher and normal Low number (according to regulation) Milk in company

17 Higher number of microorganisms Bad hygiene; No pipline desinfection; Hygiene of the farms;

18 Between extreme and limits

19 Good quality Good hygiene Good ventilation Pipeline in good conditions

20 Bacteriocins activity 576 samples are tested; 10 % are positive Indicators: Staphilococcus aureus Listeria innocua Bacillus cerus Lactococcus lactis Lactobacillus sakei

21 Presentations Week Science in Kosovo Two oral presentations: Ibrahim Mehmeti and Ibadete Krasniqi Conference in AlbShkenca organized in Macedonia Ibadete Krasniqi oral presentation and Poster

22 Future work

23 Samples collection company Skenderaj Podujeva Istogu Sharri Rahovec Lipjanë Skenderaj Istogu Rahoveci Gjakova Theranda Ferizaji Sharri Sharri

24 Analysis of dairy products

25 Workshops Two workshops next summer organized with farmers and company; Results presentation; Gust from Norway and Kosovo

26 Equipments

27 Thank you for your attention

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